Abstract
Abstract Endophytic bacteria colonizing the internal tissues of plants are being studied for its potential beneficial properties including antibacterial activity. The aims of this study were to identify the endophytic bacteria residing in the leaf of Ficus minahassae , a native plant of North Sulawesi, as well as their antibacterial activities against laboratory-adapted reference bacterial strains, Staphylococcus aureus , and Escherichia coli. The isolate identification was conducted using the 16S rRNA gene marker and the antibacterial activity test was done using a well diffusion method. A yellow-pigmented (designated as YL1) and a beige-pigmented (designated as YL2) bacteria were isolated from the internal tissue of langusei. Using the BLAST search, the isolate YL1 had a 100% identity with Brachybacterium muris , while YL2 has 99% identity with Pseudacidovorax intermedius. Ez-Taxon database confirmed that YL1 was B. muris with 99.81% similarity, while YL2 was P. intermedius with 99.80% similarity. Isolate YL2 was able to inhibit the growth of S. aureus but not E. coli. Isolate YL1 did not inhibit both reference bacteria. This study represents the first investigation of endophytic bacteria isolated from langusei leaf and their antibacterial activity.
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Claims 6
Claim · model-written summary
A yellow-pigmented isolate YL1 and a beige-pigmented isolate YL2 were isolated from the internal tissue of langusei (Ficus minahassae).
- Taxon
- Ficus minahassae
Ficus minahassaee1 - Organ / stage
- internal tissuee0
- Outcome
- yellow-pigmented (designated as YL1) and a beige-pigmented (designated as YL2) bacteria were isolatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A yellow-pigmented (designated as YL1) and a beige-pigmented (designated as YL2) bacteria were isolated from the internal tissue of langusei”
e1“residing in the leaf of Ficus minahassae”
Claim · model-written summary
Isolate YL1 had 100% BLAST identity with Brachybacterium muris, confirmed at 99.81% similarity by the Ez-Taxon database.
- Taxon
- Ficus minahassaeFicus minahassaee1
- Interactor
- Brachybacterium murisBrachybacterium murise0symbiont
- Outcome
- identitye0
- Value
- 100% identitye0
- Qualifier
- 99.81% similaritye2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the isolate YL1 had a 100% identity with Brachybacterium muris”
e2“YL1 was B. muris with 99.81% similarity”
e1“residing in the leaf of Ficus minahassae”
Claim · model-written summary
Isolate YL2 had 99% identity with Pseudacidovorax intermedius, confirmed at 99.80% similarity by the Ez-Taxon database.
- Taxon
- Ficus minahassaeFicus minahassaee1
- Interactor
- Pseudacidovorax intermediusPseudacidovorax intermediuse0symbiont
- Outcome
- identitye0
- Value
- 99% identitye0
- Qualifier
- 99.80% similaritye2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“YL2 has 99% identity with Pseudacidovorax intermedius”
e2“YL2 was P. intermedius with 99.80% similarity”
e1“residing in the leaf of Ficus minahassae”
Claim · model-written summary
Isolate YL2 was able to inhibit the growth of S. aureus.
- Taxon
- Ficus minahassaeFicus minahassaee1
- Interactor
- S. aureus
Staphylococcus aureuse0other - Outcome
- inhibit the growthe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isolate YL2 was able to inhibit the growth of S. aureus”
e1“residing in the leaf of Ficus minahassae”
Claim · model-written summary
Isolate YL2 did not inhibit the growth of E. coli.
- Taxon
- Ficus minahassaeFicus minahassaee1
- Interactor
- E. coli
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Escherichia colie0other - Outcome
- inhibit the growthe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Isolate YL2 was able to inhibit the growth of S. aureus but not E. coli”
e1“residing in the leaf of Ficus minahassae”
Claim · model-written summary
Isolate YL1 did not inhibit either reference bacterium.
- Taxon
- Ficus minahassaeFicus minahassaee1
- Interactor
- reference bacteriae0other
- Outcome
- did not inhibit both reference bacteriae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isolate YL1 did not inhibit both reference bacteria”
e1“residing in the leaf of Ficus minahassae”