Abstract
Potyviruses affect yield quantity and quality of solanaceous crops and a better understanding of their molecular properties is important for adequate disease management. Occurrence and molecular characterization of potyviruses associated with three solanaceous crops were studied. Thirty-four naturally infected leaves of pepper (Capsicum annuum L.), tomato (Solanum lycopersicum L.) and African eggplant (S. aethiopicum L.) were obtained and presence of potyviruses determined using degenerate primers targeted at the virus coat protein (CP) gene. Products were sequenced, and identities determined using the nucleotide option of the Basic Local Alignment Search Tool. Relationships between isolates were derived from phylogenetic trees using neighbor-joining algorithms. Positive amplifications were obtained only from pepper and tomato samples while sequence verification of amplicons identified only Pepper veinal mottle virus (PVMV) from eight isolates. Nucleotide and amino acid identities of PVMV CP gene ranged from 91% to 98% and 98% to 100%, respectively, confirming low genetic diversity within virus populations. Phylogenetic analyses clustered isolates into two sub-clades, indicating the presence of distinct PVMV strains. New CP-associated polymorphisms were identified in PVMV and the virus was established as the most prevalent naturally occurring potyvirus with low genetic diversity in the infected solanaceous crops. This information can be used toward the development of adequate management strategies for potyvirus infections in solanaceous crops.
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Claims 9
Claim · model-written summary
Positive potyvirus amplifications were obtained only from pepper and tomato samples.
PotyvirusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Positive amplifications were obtained only from pepper and tomato samples”
e2“presence of potyviruses determined using degenerate primers targeted at the virus coat protein (CP) gene”
Claim · model-written summary
Only Pepper veinal mottle virus was identified from eight isolates.
- Taxon
- pepper and tomatoe1
- Interactor
- Pepper veinal mottle virus (PVMV)Pepper veinal mottle viruse3pathogen
- Outcome
- identifiede3
- Value
- eight isolatese3
Verbatim
e1“Positive amplifications were obtained only from pepper and tomato samples”
e3“sequence verification of amplicons identified only Pepper veinal mottle virus (PVMV) from eight isolates”
Claim · model-written summary
Nucleotide identities of the PVMV CP gene ranged from 91% to 98%.
Verbatim
e0“potyviruses associated with three solanaceous crops”
e4“Nucleotide and amino acid identities of PVMV CP gene ranged from 91% to 98%”
Claim · model-written summary
Amino acid identities of the PVMV CP gene ranged from 98% to 100%.
Verbatim
e0“potyviruses associated with three solanaceous crops”
e5“Nucleotide and amino acid identities of PVMV CP gene ranged from 91% to 98% and 98% to 100%, respectively, confirming low genetic diversity within virus populations”
Claim · model-written summary
The PVMV population showed low genetic diversity.
Verbatim
e0“potyviruses associated with three solanaceous crops”
e6“confirming low genetic diversity within virus populations”
Claim · model-written summary
Phylogenetic analysis clustered PVMV isolates into two sub-clades, indicating distinct strains.
Verbatim
e0“potyviruses associated with three solanaceous crops”
e7“Phylogenetic analyses clustered isolates into two sub-clades, indicating the presence of distinct PVMV strains”
Claim · model-written summary
New CP-associated polymorphisms were identified in PVMV.
Verbatim
e0“potyviruses associated with three solanaceous crops”
e1“New CP-associated polymorphisms were identified in PVMV”
Claim · model-written summary
PVMV was the most prevalent naturally occurring potyvirus in the sampled crops.
- Taxon
- solanaceous cropse0
- Interactor
- Pepper veinal mottle virus (PVMV)Pepper veinal mottle viruse2pathogen
- Outcome
- most prevalent naturally occurring potyviruse1
Verbatim
e0“potyviruses associated with three solanaceous crops”
e1“the virus was established as the most prevalent naturally occurring potyvirus”
e2“sequence verification of amplicons identified only Pepper veinal mottle virus (PVMV) from eight isolates”
Claim · model-written summary
Adequate management strategies for potyvirus infections in solanaceous crops can be developed from this information.
Verbatim
e1“This information can be used toward the development of adequate management strategies for potyvirus infections in solanaceous crops”