Abstract
Seven different methods of artificial inoculation such as 1. Carborundum injury, 2. Pin prick injury 3. Sand paper injury, 4. Syringe inoculation on lower surface of leaf without needle, 5. Syringe inoculation of veins on lower surface of leaf with needle, 6. Tooth picks inoculation on collar region and 7. Pressurized spray inoculation were evaluated to find out the efficient and precise screening method for cotton bacterial blight caused by Xanthomonas citri pv. malvacearum under controlled conditions (plant growth chamber). Inoculated seedlings were incubated at 28°C, 90% RH and 3000 LUX light intensity during day time and 22°C, 90% RH and absence of light during night time for symptom development. Among them, pin prick injury recorded maximum PDI (64.25) in 20-24 days post inoculation followed by sand paper injury (56.50 PDI) in 23-27 days post inoculation on 20 day old LRA 5166 cotton seedlings compared to other methods. Both these methods developed all types of symptoms. Initial symptom of water soaked lesion was appeared in 7-8 days post inoculation in pin prick injury while it was 9-10 days in sand paper injury.
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Claims 5
Claim · model-written summary
Pin prick injury produced the maximum bacterial blight PDI of 64.25 at 20–24 days after inoculation in LRA 5166 cotton seedlings.
- Taxon
- Cotton
Gossypium hirsutumt0 - Material
- LRA 5166e0
- Organ / stage
- 20 day olde0
- Treatment
- pin prick injurye0
- Outcome
- PDIe0
- Direction
- maximume0
- Value
- 64.25e0
- Qualifier
- 20-24 days post inoculatione0
- Comparator
- other methodse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton Bacterial Blight”
e0“pin prick injury recorded maximum PDI (64.25) in 20-24 days post inoculation followed by sand paper injury (56.50 PDI) in 23-27 days post inoculation on 20 day old LRA 5166 cotton seedlings compared to other methods”
Claim · model-written summary
Sand paper injury produced a PDI of 56.50 at 23–27 days after inoculation, ranking after pin prick injury.
- Taxon
- CottonGossypium hirsutumt0
- Material
- LRA 5166e0
- Organ / stage
- 20 day olde0
- Treatment
- sand paper injurye0
- Outcome
- PDIe0
- Direction
- followed bye0
- Value
- 56.50 PDIe0
- Qualifier
- 23-27 days post inoculatione0
- Comparator
- other methodse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton Bacterial Blight”
e0“pin prick injury recorded maximum PDI (64.25) in 20-24 days post inoculation followed by sand paper injury (56.50 PDI) in 23-27 days post inoculation on 20 day old LRA 5166 cotton seedlings compared to other methods”
Claim · model-written summary
Pin prick and sand paper injury methods each developed all types of bacterial blight symptoms.
- Taxon
- CottonGossypium hirsutumt0
- Outcome
- all types of symptomse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton Bacterial Blight”
e0“Both these methods developed all types of symptoms.”
Claim · model-written summary
Initial water soaked lesions appeared 7–8 days after pin prick inoculation.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- pin prick injurye0
- Outcome
- water soaked lesione0
- Value
- 7-8 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton Bacterial Blight”
e0“Initial symptom of water soaked lesion was appeared in 7-8 days post inoculation in pin prick injury while it was 9-10 days in sand paper injury.”
Claim · model-written summary
Initial water soaked lesions appeared 9–10 days after sand paper inoculation.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- sand paper injurye0
- Outcome
- water soaked lesione0
- Value
- 9-10 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cotton Bacterial Blight”
e0“Initial symptom of water soaked lesion was appeared in 7-8 days post inoculation in pin prick injury while it was 9-10 days in sand paper injury.”