Paper · 2020 · Journal of Nematology

Compatibility of fluazaindolizine and oxamyl with Pasteuria penetrans on spore attachment to juveniles of Meloidogyne javanica and M. incognita

Eleni Nasiou; Tim Thoden; Iro V. Pardavella; et al.

2
claims
9
verbatim quotes
5
citations
1
research areas
✓
Open access · cc-by

Abstract

Abstract Fluazaindolizine is a novel sulfonamide nematicide that is the active ingredient (a.i.) of Salibro™, a.i. Reklemel™. Its compatibility with Pasteuria penetrans , a bacterial parasite of root-knot nematodes ( Meloidogyne spp.), was investigated in populations of M. javanica and M. incognita . Spores of a single P. penetrans isolate ( Pp 3) or a blend of six isolates were incubated in the suspensions of fluazaindolizine (Salibro TM 500SC, at 5, 50, and 250 ppm a.i.) and oxamyl (Vydate™ 10 L, 10% (a.i.) at 25 and 50 ppm a.i.) for 1, 7, and 21 days; controls were incubated in water. Thereafter, the suspensions were washed through a cellulose filter (3 µm) so as to remove the nematicide, and the spores retained on the filter were suspended in water. Juveniles (J2) were exposed in these spore suspensions in Petri dishes and the number of attached spores was recorded. Neither fluazaindolizine nor oxamyl, at all the tested dosages, had any negative effect on the rate of spore attachment. The spore encumbered J2 from some experiments were used to infect tomatoes. Females without egg masses were extracted from the roots after 50 days and checked for eggs in ovaries and mature spores of P. penetrans . Despite no mature spores present in the females, there was evidence of a low percentage of infection in a few treatments. A possible explanation is that since the bacterium had been kept stored in the form of dried roots for a long period, its ability to infect nematodes was decreased.

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Claims 2

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Fluazaindolizine and oxamyl at all tested dosages had no negative effect on the rate of Pasteuria penetrans spore attachment to Meloidogyne juveniles.

Treatment
fluazaindolizine (Salibro TM 500SC, at 5, 50, and 250 ppm a.i.)e3
Interactor
Pasteuria penetransPasteuria penetranse6biocontrol_agent
Second interactor
M. javanicaMeloidogyne javanicae5pest
Outcome
rate of spore attachmente1
Direction
no_changeinferrede1
Comparator
controlse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Neither fluazaindolizine nor oxamyl, at all the tested dosages, had any negative effect on the rate of spore attachment”

✓ located verbatim in the source · abstract, characters 883–1002 · show in text ↑
e2

“used to infect tomatoes”

✓ located verbatim in the source · abstract, characters 1055–1078 · show in text ↑
e3

“fluazaindolizine (Salibro TM 500SC, at 5, 50, and 250 ppm a.i.)”

✓ located verbatim in the source · abstract, characters 414–477 · show in text ↑
e4

“controls were incubated in water”

✓ located verbatim in the source · abstract, characters 561–593 · show in text ↑
e5

“populations of M. javanica and M. incognita”

✓ located verbatim in the source · abstract, characters 256–299 · show in text ↑
e6

“Pasteuria penetrans , a bacterial parasite of root-knot nematodes”

✓ located verbatim in the source · abstract, characters 149–214 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

A low percentage of Pasteuria penetrans infection was evident in tomato in a few treatments, although no mature spores were present in the females.

Interactor
Pasteuria penetransPasteuria penetranse6biocontrol_agent
Outcome
low percentage of infectione7
Qualifier
in a few treatmentse7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“used to infect tomatoes”

✓ located verbatim in the source · abstract, characters 1055–1078 · show in text ↑
e6

“Pasteuria penetrans , a bacterial parasite of root-knot nematodes”

✓ located verbatim in the source · abstract, characters 149–214 · show in text ↑
e7

“Despite no mature spores present in the females, there was evidence of a low percentage of infection in a few treatments”

✓ located verbatim in the source · abstract, characters 1219–1339 · show in text ↑