Abstract
Fusarium wilt of bitter gourd is caused by Fusarium spp. Ten isolates of Fusarium were isolated from bitter gourd growing areas of Coimbatore district to study the cultural and morphological characters of Fusarium sp. From the survey, it was found that the highest PDI was recorded in Naatukalpalayam 1 with 52.63%. The mycelial diameter of ten isolates was ranged from 6.2 to 9.0 cm at seven days of incubation on PDA. The highest colony diameter of 9.0cm was observed in BF 2 and BF 7 followed by BF5, BF8, BF9, BF4, BF6, BF10 and BF3. Minimum colony diameter was observed in BF1 with 6.1cm. Different media studies have shown that Fusarium solani could grow well in PDA and PSA. All ten isolates have produced micro, macroconidia and chlamydospores. The size (µm) of microconidia ranged from (9.2-11.8) x (3.2-4.6) and macroconidia is (15.7-24.4) x (3.8 – 5.0) and they are hyaline in color. There are 2-4 septations in macroconidia. Macroconidia are sickle shaped with pointed tips and microconidia are elongated oval in shape. Chlamydospores were seen on intercalary, terminal portion of mycelium and oval in shape. PCR amplification with universal primers of ITS1 and ITS4 yielded approximately at 560 bp. Sequencing of ITS regions followed by BLAST search confirmed the associated fungus as Fusarium solani.
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Claims 14
Claim · model-written summary
The highest wilt PDI was 52.63% at Naatukalpalayam 1.
- Taxon
- Bitter gourd
Momordica charantiat0 - Outcome
- PDIe0
- Direction
- higheste0
- Value
- 52.63%e0
- Setting
- Naatukalpalayam 1e0
FusariumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest PDI was recorded in Naatukalpalayam 1 with 52.63%.”
t0“Fusarium wilt of Bitter gourd in Coimbatore Region”
Claim · model-written summary
The ten isolates had mycelial diameters of 6.2 to 9.0 cm after seven days on PDA.
- Taxon
- Bitter gourdMomordica charantiat0
- Outcome
- mycelial diameter of ten isolatese0
- Value
- 6.2 to 9.0 cme0
- Qualifier
- seven days of incubation on PDAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mycelial diameter of ten isolates was ranged from 6.2 to 9.0 cm at seven days of incubation on PDA.”
t0“Fusarium wilt of Bitter gourd”
Claim · model-written summary
BF 2 and BF 7 had the largest colony diameter of 9.0cm.
- Taxon
- Bitter gourdMomordica charantiat0
- Material
- BF 2 and BF 7e0
- Outcome
- colony diametere0
- Direction
- higheste0
- Value
- 9.0cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest colony diameter of 9.0cm was observed in BF 2 and BF 7”
t0“Fusarium wilt of Bitter gourd”
Claim · model-written summary
BF1 had the smallest colony diameter, 6.1cm.
- Taxon
- Bitter gourdMomordica charantiat0
- Material
- BF1e0
- Outcome
- colony diametere0
- Direction
- Minimume0
- Value
- 6.1cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Minimum colony diameter was observed in BF1 with 6.1cm.”
t0“Fusarium wilt of Bitter gourd”
Claim · model-written summary
Fusarium solani grew well on PDA and PSA.
- Taxon
- Bitter gourdMomordica charantiat0
- Interactor
- Fusarium solani
_Sacc._5393379.jpg?width=60)
Fusarium solanie0pathogen - Outcome
- grow well in PDA and PSAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Sacc._5393379.jpg?width=500)
Verbatim
e0“Fusarium solani could grow well in PDA and PSA.”
t0“Bitter gourd”
Claim · model-written summary
All ten isolates produced microconidia, macroconidia and chlamydospores.
- Taxon
- Bitter gourdMomordica charantiat0
- Outcome
- produced micro, macroconidia and chlamydosporese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All ten isolates have produced micro, macroconidia and chlamydospores.”
t0“Fusarium wilt of Bitter gourd”
Claim · model-written summary
Microconidia measured (9.2-11.8) x (3.2-4.6) µm.
- Taxon
- Bitter gourdMomordica charantiat0
- Outcome
- size (µm) of microconidiae0
- Value
- (9.2-11.8) x (3.2-4.6)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The size (µm) of microconidia ranged from (9.2-11.8) x (3.2-4.6)”
t0“Fusarium wilt of Bitter gourd”
Claim · model-written summary
Macroconidia measured (15.7-24.4) x (3.8 – 5.0) µm and were hyaline.
- Taxon
- Bitter gourdMomordica charantiat0
- Outcome
- macroconidiae0
- Value
- (15.7-24.4) x (3.8 – 5.0)e0
- Qualifier
- hyaline in colore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“macroconidia is (15.7-24.4) x (3.8 – 5.0) and they are hyaline in color.”
t0“Fusarium wilt of Bitter gourd”
Claim · model-written summary
Macroconidia had two to four septations.
- Taxon
- Bitter gourdMomordica charantiat0
- Outcome
- septations in macroconidiae0
- Value
- 2-4 septationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There are 2-4 septations in macroconidia.”
t0“Fusarium wilt of Bitter gourd”
Claim · model-written summary
Macroconidia were sickle shaped with pointed tips.
- Taxon
- Bitter gourdMomordica charantiat0
- Outcome
- sickle shaped with pointed tipse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Macroconidia are sickle shaped with pointed tips”
t0“Fusarium wilt of Bitter gourd”
Claim · model-written summary
Microconidia were elongated oval.
- Taxon
- Bitter gourdMomordica charantiat0
- Outcome
- elongated oval in shapee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“microconidia are elongated oval in shape.”
t0“Fusarium wilt of Bitter gourd”
Claim · model-written summary
Chlamydospores were oval and occurred at intercalary and terminal mycelial positions.
- Taxon
- Bitter gourdMomordica charantiat0
- Outcome
- intercalary, terminal portion of mycelium and oval in shapee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chlamydospores were seen on intercalary, terminal portion of mycelium and oval in shape.”
t0“Fusarium wilt of Bitter gourd”
Claim · model-written summary
ITS primer amplification yielded an approximately 560 bp product.
- Taxon
- Bitter gourdMomordica charantiat0
- Gene
- ITS1 and ITS4e0
- Outcome
- PCR amplificatione0
- Value
- 560 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCR amplification with universal primers of ITS1 and ITS4 yielded approximately at 560 bp.”
t0“Fusarium wilt of Bitter gourd”
Claim · model-written summary
ITS sequencing and BLAST identified the associated fungus as Fusarium solani.
- Taxon
- Bitter gourdMomordica charantiat0
- Interactor
- Fusarium solaniFusarium solanie0pathogen
- Outcome
- confirmed the associated fungus as Fusarium solanie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequencing of ITS regions followed by BLAST search confirmed the associated fungus as Fusarium solani.”
t0“Bitter gourd”