Abstract
Monilinia laxa is a necrotrophic plant pathogen able to infect and produce substantial losses on stone fruit. Three different isolates of M. laxa were characterized according to their aggressiveness on nectarines. M. laxa 8L isolate was the most aggressive on fruit, 33L isolate displayed intermediated virulence level, and 5L was classified as a weak aggressive isolate. Nectarine colonization process by the weak isolate 5L was strongly delayed. nLC-MS/MS proteomic studies using in vitro peach cultures provided data on exoproteomes of the three isolates at equivalent stages of brown rot colonization; 3 days for 8L and 33L, and 7 days for 5L. A total of 181 proteins were identified from 8L exoproteome and 289 proteins from 33L at 3 dpi, and 206 proteins were identified in 5L exoproteome at 7 dpi. Although an elevated number of proteins lacked a predicted function, the vast majority of proteins belong to OG group “metabolism”, composed of categories such as "carbohydrate transport and metabolism" in 5L, and "energy production and conversion" most represented in 8L and 33L. Among identified proteins, 157 that carried a signal peptide were further examined and classified. Carbohydrate-active enzymes and peptidases were the main groups revealing different protein alternatives with the same function among isolates. Our data suggested a subset of secreted proteins as possible markers of differential virulence in more aggressive isolates, MlPG1 MlPME3, NEP-like, or endoglucanase proteins. A core-exoproteome among isolates independently of their virulence but time-dependent was also described. This core included several well-known virulence factors involved in host-tissue factors like cutinase, pectin lyases and acid proteases. The secretion patterns supported the assumption that M. laxa deploys an extensive repertoire of proteins to facilitate the host infection and colonization and provided information for further characterization of M. laxa pathogenesis.
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Claims 15
Claim · model-written summary
Isolate 8L was the most aggressive on nectarine fruit.
- Taxon
- Peach
Prunus persicat0 - Material
- 8L isolatee0
- Interactor
- M. laxa
Monilinia laxae0pathogen - Outcome
- most aggressive on fruite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“M. laxa 8L isolate was the most aggressive on fruit”
t0“Peach Tissue”
Claim · model-written summary
Isolate 33L had intermediate virulence on nectarine fruit.
- Taxon
- PeachPrunus persicat0
- Material
- 33L isolatee0
- Outcome
- intermediated virulence levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“33L isolate displayed intermediated virulence level”
t0“Peach Tissue”
Claim · model-written summary
Isolate 5L was weakly aggressive on nectarine fruit.
- Taxon
- PeachPrunus persicat0
- Material
- 5Le0
- Outcome
- weak aggressive isolatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5L was classified as a weak aggressive isolate”
t0“Peach Tissue”
Claim · model-written summary
Nectarine colonization by isolate 5L was strongly delayed.
- Taxon
- NectarinePrunus persica var. nucipersicae0
- Material
- 5Le0
- Outcome
- Nectarine colonization processe0
- Direction
- strongly delayede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nectarine colonization process by the weak isolate 5L was strongly delayed”
Claim · model-written summary
The 8L exoproteome contained 181 identified proteins at 3 dpi.
- Taxon
- PeachPrunus persicat0
- Material
- 8Le0
- Outcome
- proteins were identified from 8L exoproteomee0
- Value
- 181 proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 181 proteins were identified from 8L exoproteome”
t0“Peach Tissue”
Claim · model-written summary
The 33L exoproteome contained 289 identified proteins at 3 dpi.
- Taxon
- PeachPrunus persicat0
- Material
- 33Le0
- Outcome
- proteins from 33Le0
- Value
- 289 proteinse0
- Qualifier
- 3 dpie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“289 proteins from 33L at 3 dpi”
t0“Peach Tissue”
Claim · model-written summary
The 5L exoproteome contained 206 identified proteins at 7 dpi.
- Taxon
- PeachPrunus persicat0
- Material
- 5Le0
- Outcome
- proteins were identified in 5L exoproteomee0
- Value
- 206 proteinse0
- Qualifier
- 7 dpie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“206 proteins were identified in 5L exoproteome at 7 dpi”
t0“Peach Tissue”
Claim · model-written summary
Most identified exoproteins belonged to the metabolism OG group.
- Taxon
- PeachPrunus persicat0
- Outcome
- vast majority of proteins belong to OG group “metabolism”e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the vast majority of proteins belong to OG group “metabolism””
t0“Peach Tissue”
Claim · model-written summary
Carbohydrate transport and metabolism was represented in isolate 5L.
- Taxon
- PeachPrunus persicat0
- Material
- 5Le0
- Outcome
- "carbohydrate transport and metabolism"e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“"carbohydrate transport and metabolism" in 5L”
t0“Peach Tissue”
Claim · model-written summary
Energy production and conversion was most represented in isolates 8L and 33L.
- Taxon
- PeachPrunus persicat0
- Material
- 8L and 33Le0
- Outcome
- "energy production and conversion" most representede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“"energy production and conversion" most represented in 8L and 33L”
t0“Peach Tissue”
Claim · model-written summary
Carbohydrate-active enzymes and peptidases showed different alternatives with the same function among isolates.
- Taxon
- PeachPrunus persicat0
- Gene
- Carbohydrate-active enzymes and peptidasese0
- Outcome
- different protein alternatives with the same function among isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Carbohydrate-active enzymes and peptidases were the main groups revealing different protein alternatives with the same function among isolates”
t0“Peach Tissue”
Claim · model-written summary
Secreted MlPG1, MlPME3, NEP-like and endoglucanase proteins were proposed as markers of higher virulence.
- Taxon
- PeachPrunus persicat0
- Gene
- MlPG1 MlPME3, NEP-like, or endoglucanase proteinse0
- Outcome
- differential virulence in more aggressive isolatese0
- Qualifier
- possible markerse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our data suggested a subset of secreted proteins as possible markers of differential virulence in more aggressive isolates, MlPG1 MlPME3, NEP-like, or endoglucanase proteins”
t0“Peach Tissue”
Claim · model-written summary
A time-dependent core exoproteome was shared among isolates regardless of virulence.
- Taxon
- PeachPrunus persicat0
- Outcome
- core-exoproteome among isolatese0
- Qualifier
- independently of their virulence but time-dependente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A core-exoproteome among isolates independently of their virulence but time-dependent was also described”
t0“Peach Tissue”
Claim · model-written summary
The core exoproteome included cutinase, pectin lyases and acid proteases.
- Taxon
- PeachPrunus persicat0
- Gene
- cutinase, pectin lyases and acid proteasese0
- Outcome
- virulence factors involved in host-tissue factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This core included several well-known virulence factors involved in host-tissue factors like cutinase, pectin lyases and acid proteases”
t0“Peach Tissue”
Claim · model-written summary
Secretion patterns supported an extensive M. laxa protein repertoire facilitating host infection and colonization.
- Taxon
- PeachPrunus persicat0
- Interactor
- M. laxaMonilinia laxae0pathogen
- Outcome
- host infection and colonizatione0
- Qualifier
- extensive repertoire of proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The secretion patterns supported the assumption that M. laxa deploys an extensive repertoire of proteins to facilitate the host infection and colonization”
t0“Peach Tissue”