Abstract
Abstract Six species of the genus Bursaphelenchus were recovered as the result of a survey to assess the potential introduction of Bursaphelenchus xylophilus into the conifer forests of Romania. Bursaphelenchus xylophilus was not detected in the sampling regions. Bursaphelenchus dietrichi was recovered from one point, representing the first report of this species in the country. The other detected species, B . abietinus , B . andrassyi , B . hofmanni , B . fraudulentus and B . willibaldi, were characterized by their morphometric data and molecular analyses. The morphometrics of Romanian populations of B . hofmanni and B . willibaldi are presented for the first time. The phylogenetic relationships of the recovered species (except for B . abietinus , with no successful sequencing) were reconstructed using the sequences of the D2‐D3 expansion segments of the large subunit of ribosomal DNA (D2‐D3LSU rDNA). Some of the species were also characterized using the restriction fragment length polymorphism (RFLP) pattern of the internal transcribed spacer (ITS) fragment. The distribution, related host trees and phylogenetic relationships of the identified species are discussed.
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Claims 5
Claim · model-written summary
A survey of conifers in Romania recovered six species of the genus Bursaphelenchus.
- Interactor
- Bursaphelenchus
Bursaphelenchuse0other - Outcome
- Six species of the genus Bursaphelenchus were recoverede0
- Value
- Six speciese0
- Setting
- Romaniat1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“conifers”
t1“Romania”
e0“Six species of the genus Bursaphelenchus were recovered as the result of a survey”
Claim · model-written summary
Bursaphelenchus xylophilus was not detected in the sampled regions of Romanian conifer forests.
- Interactor
- Bursaphelenchus xylophilus
Bursaphelenchus xylophiluse0pathogen - Outcome
- was not detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“conifers”
e0“Bursaphelenchus xylophilus was not detected in the sampling regions”
Claim · model-written summary
Bursaphelenchus dietrichi was recovered from one sampling point, the first report of this species in Romania.
Verbatim
t0“conifers”
e0“Bursaphelenchus dietrichi was recovered from one point, representing the first report of this species in the country”
Claim · model-written summary
Morphometric data of Romanian populations of B. hofmanni are presented for the first time.
- Interactor
- B . hofmanniBursaphelenchus hofmannie0other
- Outcome
- morphometricse0
- Qualifier
- presented for the first timee0
Verbatim
t0“conifers”
e0“The morphometrics of Romanian populations of B . hofmanni and B . willibaldi are presented for the first time”
Claim · model-written summary
Morphometric data of Romanian populations of B. willibaldi are presented for the first time.
- Interactor
- B . willibaldiBursaphelenchus willibaldie0other
- Outcome
- morphometricse0
- Qualifier
- presented for the first timee0
Verbatim
t0“conifers”
e0“The morphometrics of Romanian populations of B . hofmanni and B . willibaldi are presented for the first time”