Abstract
ABSTRACT In the present study twelve plant associated bacteria which isolated from root nodules of C. arietinum L. collected from Central-Anatolia region of Turkey were identified with current molecular techniques. None of the isolates were produced root nodules or showed pathogenic effects (gall or hairy root) on the original host as the result of authentication and pathogenicity tests, respectively. These results have suggested that all these isolates are root endophytic bacteria. Additionally, PCR amplifications for nodD and virA genes did not revealed any bands. These results showed that these isolates have not harbour symbiotic (pSym) or pathogenicity (Ti: Tumor inducing or Ri: Root inducing) plasmids which are necessary for nodulation or virulence, respectively. TP-RAPD analysis revealed three patterns indicating three genetically distinct group within the isolate collection. From each pattern one representative isolate was selected for further molecular analyses. Phylogenetic analyses depending on nucleotide sequences of 16S rDNA and recA genes assigned representative isolates of Pattern-A (n: 4) and Pattern-B (n: 6) to A. radiobacter. On the other hand, the representative isolate of Pattern-C (n: 2) appeared as related to A. nepotum. As a result, this study presents the first phylogenetically identified root-endophytic Agrobacterium radiobacter and A. nepotum isolates from root nodules of C. arietinum L. grown in Central Anatolia part of Turkey. Additionally the first molecular data of A. radiobacter for Turkey also presented. ÖZ Bu çalışmada Türkiye’nin İç Anadolu bölgesinden toplanan C. arietinum L. bitkisinin kök nodüllerin’den izole edilmiş on iki bitki ilişkili bakteri moleküler teknikler kullanılarak teşhis edilmiştir. Bu izolatların hiç birisi otantikasyon ve patojenite testlerinin sonucunda orijinal konak üzerinde nodül oluşturmamış veya patojenik etki (gall oluşumu veya saçak kök oluşumu) göstermemiştir. Bu sonuçlar bütün bu izolatların kök endofitik bakteriler olduğunu ortaya koymuştur. Ek olarak nodD ve virA genleri için yapılan PCR amplifikasyonları herhangi bir bant ortaya koymamıştır. Bu sonuçlar bu izolatların nodülasyon veya virülans için gerekli olan sırasıyla simbiyotik (pSym) veya patojenite (Ti: Tümör oluşturan veya Ri: Saçak kök oluşturan) pilazmitlerini taşımadıklarını göstermiştir. TP-RAPD analizi izolat kolleksiyonu içerisinde genetik olarak ayrı üç gruba işaret eden üç şablon ortaya koymuştur. İleri moleküler analizler için her şablondan bir temsilci izolat seçilmiştir. 16S rDNA ve recA genlerinin nükleotid dizilerine dayalı filogenetik analizler Şablon-A (n: 4) ve Şablon-B’nin (n: 6) temsilci izolatlarını Agrobacterium radiobacter türü ile ilişkilendirmiştir. Diğer taraftan, Şablon-C’nin (n: 2) temsilci izolatı A. nepotum olarak ortaya çıkmıştır. Sonuç olarak bu çalışma Türkiye’nin İç Anadolu Bölgesinde yetiştirilen C. arietinum L.’nin kök nodüllerin’den filogenetik olarak tanımlanmış ilk kök endofitik Agrobacterium radiobacter and A. nepotum izolatlarını sunmaktadır. Ek olarak, A. radiobacter için Türkiye’den ilk moleküler verileri de sunmaktadır.
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Claims 9
Claim · model-written summary
None of the isolates produced nodules or pathogenic gall or hairy-root effects on the original host.
- Taxon
- C. arietinum L.e1
- Treatment
- isolatese0
- Interactor
- plant associated bacteriae1other
- Outcome
- root nodules or showed pathogenic effects (gall or hairy root)e0
- Direction
- Nonee0
- Setting
- Central-Anatolia region of Turkeye1
Not reportedComparator
Verbatim
e0“None of the isolates were produced root nodules or showed pathogenic effects (gall or hairy root) on the original host as the result of authentication and pathogenicity tests, respectively.”
e1“twelve plant associated bacteria which isolated from root nodules of C. arietinum L. collected from Central-Anatolia region of Turkey”
Claim · model-written summary
All isolates were interpreted as root endophytic bacteria.
- Taxon
- Agrobacterium spp.
.jpg?width=60)
Agrobacteriumt0 - Outcome
- root endophytic bacteriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“These results have suggested that all these isolates are root endophytic bacteria.”
t0“Agrobacterium spp.”
Claim · model-written summary
PCR amplification of nodD and virA yielded no bands.
- Taxon
- Agrobacterium spp.Agrobacteriumt0
- Gene
- nodD and virAe0
- Outcome
- did not revealed any bandse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Additionally, PCR amplifications for nodD and virA genes did not revealed any bands.”
t0“Agrobacterium spp.”
Claim · model-written summary
The isolates were interpreted as lacking symbiotic and pathogenicity plasmids needed for nodulation or virulence.
- Taxon
- Agrobacterium spp.Agrobacteriumt0
- Outcome
- have not harbour symbiotic (pSym) or pathogenicity (Ti: Tumor inducing or Ri: Root inducing) plasmidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results showed that these isolates have not harbour symbiotic (pSym) or pathogenicity (Ti: Tumor inducing or Ri: Root inducing) plasmids which are necessary for nodulation or virulence, respectively.”
t0“Agrobacterium spp.”
Claim · model-written summary
TP-RAPD distinguished three genetically distinct isolate groups.
- Taxon
- Agrobacterium spp.Agrobacteriumt0
- Outcome
- genetically distinct groupe0
- Value
- threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TP-RAPD analysis revealed three patterns indicating three genetically distinct group within the isolate collection.”
t0“Agrobacterium spp.”
Claim · model-written summary
Representatives of Pattern-A and Pattern-B were assigned to A. radiobacter.
- Taxon
- A. radiobacter
Agrobacterium radiobactere0 - Gene
- 16S rDNA and recAe0
- Outcome
- assigned representative isolates of Pattern-A (n: 4) and Pattern-B (n: 6) to A. radiobactere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analyses depending on nucleotide sequences of 16S rDNA and recA genes assigned representative isolates of Pattern-A (n: 4) and Pattern-B (n: 6) to A. radiobacter.”
Claim · model-written summary
The Pattern-C representative was related to A. nepotum.
- Taxon
- A. nepotumRhizobium nepotume0
- Material
- Pattern-Ce0
- Outcome
- related to A. nepotume0
Verbatim
e0“On the other hand, the representative isolate of Pattern-C (n: 2) appeared as related to A. nepotum.”
Claim · model-written summary
The study presents the first identified root-endophytic A. radiobacter isolates from chickpea nodules in Central Anatolia and the first Turkish molecular data for this species.
- Taxon
- Agrobacterium radiobacterAgrobacterium radiobactere0
- Interactor
- C. arietinum L.e0host
- Outcome
- phylogenetically identified root-endophytice0
- Qualifier
- firste0
- Setting
- Central Anatolia part of Turkeye0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As a result, this study presents the first phylogenetically identified root-endophytic Agrobacterium radiobacter and A. nepotum isolates from root nodules of C. arietinum L. grown in Central Anatolia part of Turkey. Additionally the first molecular data of A. radiobacter for Turkey also presented.”
Claim · model-written summary
The study presents the first identified root-endophytic A. nepotum isolates from chickpea nodules in Central Anatolia.
- Taxon
- A. nepotumRhizobium nepotume0
- Interactor
- C. arietinum L.e0host
- Outcome
- phylogenetically identified root-endophytice0
- Qualifier
- firste0
- Setting
- Central Anatolia part of Turkeye0
Not reportedValue
Verbatim
e0“As a result, this study presents the first phylogenetically identified root-endophytic Agrobacterium radiobacter and A. nepotum isolates from root nodules of C. arietinum L. grown in Central Anatolia part of Turkey.”