Paper · 2020 · Applications in Plant Sciences

A targeted sequence capture array for phylogenetics and population genomics in the Salicaceae

Brian J. Sanderson; Stephen P. DiFazio; Quentin C. B. Cronk; et al.

6
claims
7
verbatim quotes
13
citations
4
research areas
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Open access · cc-by-nc-nd

Abstract

Premise The family Salicaceae has proved taxonomically challenging, especially in the genus Salix , which is speciose and features frequent hybridization and polyploidy. Past efforts to reconstruct the phylogeny with molecular barcodes have failed to resolve the species relationships of many sections of the genus. Methods We used the wealth of sequence data in the family to design sequence capture probes to target regions of 300–1200 bp of exonic regions of 972 genes. Results We recovered sequence data for nearly all of the targeted genes in three species of Populus and three species of Salix . We present a species tree, discuss concordance among gene trees, and present population genomic summary statistics for these loci. Conclusions Our sequence capture array has extremely high capture efficiency within the genera Populus and Salix , resulting in abundant phylogenetic information. Additionally, these loci show promise for population genomic studies.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Sequence data were recovered for nearly all targeted genes in three Populus species.

Taxon
PopulusPopuluse0
Outcome
sequence datae0
Value
nearly alle0
Qualifier
three species of Populuse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Populus
Populus · Taxon · picture of the genus (one of its species)Christian Fischer · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“We recovered sequence data for nearly all of the targeted genes in three species of Populus and three species of Salix”

✓ located verbatim in the source · abstract, characters 481–599 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Sequence data were recovered for nearly all targeted genes in three Salix species.

Taxon
SalixSalixe0
Outcome
sequence datae0
Value
nearly alle0
Qualifier
three species of Salixe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Salix
Salix · Taxon · picture of the genus (one of its species)Bruce Marlin · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“We recovered sequence data for nearly all of the targeted genes in three species of Populus and three species of Salix”

✓ located verbatim in the source · abstract, characters 481–599 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The sequence capture array had extremely high capture efficiency in Populus.

Taxon
PopulusPopuluse0
Outcome
capture efficiencye0
Value
extremely highe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Populus
Populus · Taxon · picture of the genus (one of its species)Christian Fischer · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Our sequence capture array has extremely high capture efficiency within the genera Populus and Salix”

✓ located verbatim in the source · abstract, characters 745–845 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The sequence capture array had extremely high capture efficiency in Salix.

Taxon
SalixSalixe0
Outcome
capture efficiencye0
Value
extremely highe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Salix
Salix · Taxon · picture of the genus (one of its species)Bruce Marlin · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Our sequence capture array has extremely high capture efficiency within the genera Populus and Salix”

✓ located verbatim in the source · abstract, characters 745–845 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The array produced abundant phylogenetic information for Populus and Salix.

Taxon
Populus and SalixPopulusSalixe0
Outcome
phylogenetic informatione0
Value
abundante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Populus
Populus · Taxon · picture of the genus (one of its species)Christian Fischer · CC BY-SA 3.0 · Wikimedia Commons ↗
Salix
Salix · Taxon · picture of the genus (one of its species)Bruce Marlin · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Our sequence capture array has extremely high capture efficiency within the genera Populus and Salix , resulting in abundant phylogenetic information”

✓ located verbatim in the source · abstract, characters 745–894 · show in text ↑
Method / resourceauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

The captured loci show promise for population genomic studies.

Taxon
SalicaceaeSalicaceaet0
Outcome
population genomic studiese0
Value
show promisee0

Verbatim

e0

“these loci show promise for population genomic studies”

✓ located verbatim in the source · abstract, characters 910–964 · show in text ↑
t0

“Salicaceae”

✓ located verbatim in the source · title, characters 83–93 · show in text ↑