Abstract
Objective of this study was to isolate, sequence and analyze variations in candidate genes involved in nitrogen (N) metabolism in potato (Solanum tuberosum L.) for future breeding application. Two contrasting potato varieties, viz. Kufri Gaurav (N efficient) and Kufri Jyoti (N inefficient) were used. Of the total 17 PCR primers tested for N metabolism genes in the both varieties and only 12 distinct bands were cloned and sequenced, which were amplified by six primers of five genes (nitrate transporter-NRT, ammonium transporter-AMT, nitrate reductase-NR, nitrite reductase-NIR and asparagines synthetase-AS). Following sequence analysis, non-redundant sequences with uninterrupted open reading frames of 12 ‘N-homologous genes’ were identified to the known N metabolic pathway genes. Thus, the identified 12 N-homologous genes may serve as an important genomic resource for novel gene/marker discovery and would be useful for marker-assisted selection (MAS) in potato with better nitrogen use efficiency (NUE).
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Claims 3
Claim · model-written summary
Twelve bands from six primers spanning five nitrogen metabolism genes were cloned and sequenced.
- Taxon
- potato
Solanum tuberosume1 - Gene
- nitrate transporter-NRT, ammonium transporter-AMT, nitrate reductase-NR, nitrite reductase-NIR and asparagines synthetase-ASe0
- Outcome
- distinct bands were cloned and sequencede0
- Value
- 12 distinct bandse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only 12 distinct bands were cloned and sequenced, which were amplified by six primers of five genes (nitrate transporter-NRT, ammonium transporter-AMT, nitrate reductase-NR, nitrite reductase-NIR and asparagines synthetase-AS)”
e1“in potato (Solanum tuberosum L.)”
Claim · model-written summary
Sequence analysis identified 12 non-redundant N-homologous genes with uninterrupted open reading frames.
- Taxon
- potatoSolanum tuberosume1
- Outcome
- ‘N-homologous genes’ were identifiede0
- Value
- 12 ‘N-homologous genes’e0
- Qualifier
- uninterrupted open reading framese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“non-redundant sequences with uninterrupted open reading frames of 12 ‘N-homologous genes’ were identified to the known N metabolic pathway genes”
e1“in potato (Solanum tuberosum L.)”
Claim · model-written summary
The identified genes may be useful for gene or marker discovery and marker-assisted selection for nitrogen use efficiency.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- genomic resource for novel gene/marker discoverye0
- Value
- 12 N-homologous genese0
- Qualifier
- may servee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the identified 12 N-homologous genes may serve as an important genomic resource for novel gene/marker discovery and would be useful for marker-assisted selection (MAS) in potato with better nitrogen use efficiency (NUE)”