Abstract
Aim. The main purpose of the research was to examine distinct phytopathogenic and toxigenic properties of Fusarium isolates received from Triticum aestivum L. grains and to find out their toxicity.Methods. In this paper, the phytotoxic activity and the aggressiveness of the investigated isolates were defined in order to characterize their phytopathogenicity. In order to determine the possibility of the isolates to synthesize zearalenone, the PCR analysis was used to detect the presence of polyketide synthase gene PKS13, which is important for zearalenone biosynthesis.Results. Most of the investigated isolates were characterized by a moderate level of phytotoxic activity, with only one isolate Fusarium sp. КМА-17 being classified as toxic. Based on the analysis of the phytotoxic profile of the studied fungi cultures, their varietal specialization was established: the spring wheat Tyubalt variety was more susceptible to the phytotoxic effect compared to the winter Wdala variety. Surprisingly, metabolites from some of the isolates were observed to stimulate growth of the Wdala variety seedlings. All of the received fungi isolates caused pathological changes in the experimental plants, including tissue necrosis of shoots and roots of seedlings, retardation of growth and wilting. The studied cultures were characterized by a moderate degree of aggressiveness and were assigned to the second pathogenicity group. PCR study did not reveal the PKS13 gene in genomes of the isolates, which implies the impossibility of synthesizing zearalenone.Conclusions. To sum up, the received Fusarium sp. isolates were characterized by moderate phytotoxicity and aggressiveness and did not show any ability to synthesize zearalenone. Taking the complex of investigated features into account the Fusarium sp. isolates can be considered non-toxigenic.Manuscript received 29.04.2020
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Claims 7
Claim · model-written summary
Most Fusarium isolates had moderate phytotoxic activity.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Outcome
- phytotoxic activitye0
- Qualifier
- moderate levele0
FusariumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Triticum aestivum L.”
t1“Fusarium isolates”
e0“Most of the investigated isolates were characterized by a moderate level of phytotoxic activity”
Claim · model-written summary
Fusarium sp. КМА-17 was classified as toxic.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Interactor
- Fusarium sp. КМА-17e0pathogen
- Outcome
- Fusarium sp. КМА-17 being classified as toxice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Triticum aestivum L.”
e0“only one isolate Fusarium sp. КМА-17 being classified as toxic.”
Claim · model-written summary
Spring wheat Tyubalt was more susceptible to Fusarium phytotoxicity than winter Wdala.
- Taxon
- wheatTriticum aestivume0
- Material
- Tyubalte0
- Outcome
- susceptible to the phytotoxic effecte0
- Direction
- more susceptiblee0
- Comparator
- Wdalae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the spring wheat Tyubalt variety was more susceptible to the phytotoxic effect compared to the winter Wdala variety.”
t0“Fusarium isolates”
Claim · model-written summary
Metabolites from some Fusarium isolates stimulated Wdala seedling growth.
- Taxon
- wheatTriticum aestivume1
- Material
- Wdalae0
- Treatment
- metabolites from some of the isolatese0
- Outcome
- growth of the Wdala variety seedlingse0
- Direction
- stimulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metabolites from some of the isolates were observed to stimulate growth of the Wdala variety seedlings.”
t0“Fusarium isolates”
e1“spring wheat Tyubalt variety was more susceptible to the phytotoxic effect compared to the winter Wdala variety.”
Claim · model-written summary
All isolates caused seedling shoot and root necrosis, growth retardation and wilting.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Treatment
- fungi isolatese0
- Outcome
- tissue necrosis of shoots and roots of seedlings, retardation of growth and wiltinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Triticum aestivum L.”
e0“All of the received fungi isolates caused pathological changes in the experimental plants, including tissue necrosis of shoots and roots of seedlings, retardation of growth and wilting.”
t1“Fusarium isolates”
Claim · model-written summary
The isolates showed moderate aggressiveness and were assigned to pathogenicity group two.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Outcome
- moderate degree of aggressivenesse0
- Qualifier
- second pathogenicity groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Triticum aestivum L.”
t1“Fusarium isolates”
e0“The studied cultures were characterized by a moderate degree of aggressiveness and were assigned to the second pathogenicity group.”
Claim · model-written summary
PCR found no PKS13 gene in the isolates, suggesting no zearalenone synthesis ability.
- Taxon
- Triticum aestivum L.Triticum aestivumt0
- Gene
- PKS13e0
- Outcome
- did not reveal the PKS13 genee0
- Qualifier
- impossibility of synthesizing zearalenonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Triticum aestivum L.”
t1“Fusarium isolates”
e0“PCR study did not reveal the PKS13 gene in genomes of the isolates, which implies the impossibility of synthesizing zearalenone.”