Abstract
L.). While both have been shown to be useful to eliminate the main grapevine viruses, their efficiency differs depending on the virus and grapevine variety. In our work, we investigated the efficiency of these two virus elimination methods using small RNA high-throughput sequencing (HTS) and RT-PCR as virus diagnostics. Field grown mother plants of four clones representing three cultivars, infected with different viruses and viroids, were selected for elimination via somatic embryogenesis (SE) and meristem culture (ME). Our results show for the first time that using SE, elimination in mother plants was effective for all viruses, i.e., grapevine rupestris vein feathering virus (GRVFV), grapevine Syrah virus 1 (GSyV-1), Grapevine virus T (GVT) and grapevine Pinot gris virus (GPGV). This study also confirms previous studies showing that SE is a possible strategy for the elimination of GFkV, GRSPaV, HSVd, and GYSVd-1. Our results demonstrate that the efficacy of virus elimination via SE is relatively high while the purging of viroids is lower. Our work provides evidence that the efficiency of SE is comparable to that of the technically difficult ME technique, and that SE will offer a more effective strategy for the production of virus-free grapevine in the future.
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Claims 7
Claim · model-written summary
Somatic embryogenesis eliminated GRVFV from mother plants.
- Interactor
- grapevine rupestris vein feathering virus (GRVFV)Grapevine rupestris vein feathering viruse0pathogen
- Outcome
- elimination in mother plantse0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“using SE, elimination in mother plants was effective for all viruses, i.e., grapevine rupestris vein feathering virus (GRVFV), grapevine Syrah virus 1 (GSyV-1), Grapevine virus T (GVT) and grapevine Pinot gris virus (GPGV)”
Claim · model-written summary
Somatic embryogenesis eliminated GSyV-1 from mother plants.
- Interactor
- grapevine Syrah virus 1 (GSyV-1)Grapevine Syrah virus 1e0pathogen
- Outcome
- grapevine Syrah virus 1 (GSyV-1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“grapevine Syrah virus 1 (GSyV-1)”
Claim · model-written summary
Somatic embryogenesis eliminated GVT from mother plants.
- Interactor
- Grapevine virus T (GVT)Grapevine virus Te0pathogen
- Outcome
- Grapevine virus T (GVT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“Grapevine virus T (GVT)”
Claim · model-written summary
Somatic embryogenesis eliminated GPGV from mother plants.
- Interactor
- grapevine Pinot gris virus (GPGV)Grapevine Pinot gris viruse0pathogen
- Outcome
- grapevine Pinot gris virus (GPGV)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“grapevine Pinot gris virus (GPGV)”
Claim · model-written summary
Somatic embryogenesis can eliminate GFkV, GRSPaV, HSVd and GYSVd-1.
- Outcome
- elimination of GFkV, GRSPaV, HSVd, and GYSVd-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“SE is a possible strategy for the elimination of GFkV, GRSPaV, HSVd, and GYSVd-1”
Claim · model-written summary
Virus elimination by somatic embryogenesis was relatively high, whereas viroid purging was lower.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“the efficacy of virus elimination via SE is relatively high while the purging of viroids is lower”
Claim · model-written summary
Somatic embryogenesis was comparable to meristem culture in virus elimination efficiency.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“the efficiency of SE is comparable to that of the technically difficult ME technique”