Abstract
Barley diseases are the major yield limiting factors for barley cultivation in Nepal. Stripe/Yellow rust (P. striformis f.sp. hordei and P. striformis f.sp. tritici), leaf rust (Puccinia hordei), and crown rust (P. coronata) are the major rust diseases in Nepal. Pyramiding resistance genes against all these rust diseases are possible through molecular marker assisted breeding. Sweden originated barley variety ‘Bonus’ is found resistant to stripe rust and having linked microsatellite markers for stripe rust and crown rust resistance. Similarly, Nepalese hull-less barley variety ‘Solu Uwa’ and Nepalese awn-less barley landrace NPGR Acc# 2478 have linked microsatellite markers for leaf rust resistance. Therefore, one polymorphic sequence tagged sites (STS) marker (ABG054) for stripe rust resistance, two polymorphic simple sequence repeats (SSR) markers (Bmac0144h and HVM049) for leaf rust and one polymorphic SSR marker (Bmag0006) for crown rust resistance were used to select the advanced barley lines (at F8 stage) from above parents. Field screening of stripe rust resistance was also conducted. Among 51 advanced and field disease resistance lines from Bonus/Solu Uwa cross, we have selected 10 pyramided lines for all three types of barley rust resistance. Similarly, among 39 advanced and field disease resistance lines from Bonus/NPGR Acc#2478 cross we have selected three pyramided lines and advanced for further yield testing for general cultivation purpose. The chances of losing the desired gene are high in late generation selection using molecular marker assisted selection (MAS); but the chances of getting agronomically superior varietal output will also increase.
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Claims 6
Claim · model-written summary
Bonus was resistant to stripe rust and carried linked markers for stripe and crown rust resistance.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Material
- Bonuse0
- Outcome
- resistant to stripe rust and having linked microsatellite markers for stripe rust and crown rust resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“barley variety ‘Bonus’ is found resistant to stripe rust and having linked microsatellite markers for stripe rust and crown rust resistance”
t0“Barley”
Claim · model-written summary
Solu Uwa had linked microsatellite markers for leaf rust resistance.
- Taxon
- barleyHordeum vulgaree0
- Material
- Solu Uwae0
- Outcome
- linked microsatellite markers for leaf rust resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley variety ‘Solu Uwa’ and Nepalese awn-less barley landrace NPGR Acc# 2478 have linked microsatellite markers for leaf rust resistance”
t0“Barley”
Claim · model-written summary
NPGR Acc# 2478 had linked microsatellite markers for leaf rust resistance.
- Taxon
- barleyHordeum vulgaree0
- Material
- NPGR Acc# 2478e0
- Outcome
- linked microsatellite markers for leaf rust resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley landrace NPGR Acc# 2478 have linked microsatellite markers for leaf rust resistance”
t0“Barley”
Claim · model-written summary
Ten of 51 Bonus/Solu Uwa lines were selected for resistance to all three barley rusts.
- Taxon
- barleyHordeum vulgaret0
- Material
- Bonus/Solu Uwae0
- Outcome
- pyramided lines for all three types of barley rust resistancee0
- Value
- 10e0
- Qualifier
- Among 51 advanced and field disease resistance linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among 51 advanced and field disease resistance lines from Bonus/Solu Uwa cross, we have selected 10 pyramided lines for all three types of barley rust resistance”
t0“Barley”
Claim · model-written summary
Three of 39 Bonus/NPGR Acc#2478 lines were selected and advanced for yield testing.
- Taxon
- barleyHordeum vulgaret0
- Material
- Bonus/NPGR Acc#2478e0
- Outcome
- pyramided linese0
- Value
- threee0
- Qualifier
- among 39 advanced and field disease resistance linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“among 39 advanced and field disease resistance lines from Bonus/NPGR Acc#2478 cross we have selected three pyramided lines and advanced for further yield testing for general cultivation purpose”
t0“Barley”
Claim · model-written summary
The authors judged that late-generation marker selection increases the chance of losing desired genes while also increasing the chance of agronomically superior varieties.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- chances of losing the desired genee0
- Qualifier
- chances of getting agronomically superior varietal output will also increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The chances of losing the desired gene are high in late generation selection using molecular marker assisted selection (MAS); but the chances of getting agronomically superior varietal output will also increase”
t0“Barley”