Abstract
This research was undertaken with the aim to identify the robustness of available genetic markers (chloroplast DNA sequences and nuclear microsatellites) to differentiate G. parviflora subsp. parviflora (Gpp) from five closely related taxa within the Linearifolia Group of grevilleas; particularly G. humilis subsp. humilis (Ghh) in the Lake Macquarie area. One chloroplast region had a consistent character that separated the northern populations of Gpp (northern: Hunter area) from populations in the south of its range (southern: Cumberland Plains area) and all other taxa. This character was an 11 base pair sequence inversion. An assessment of over 50 microsatellite loci led to the identification of nine primer pairs that consistently amplified variable product in the target taxon. Genetic profiling using the microsatellites from a minimum of 10 individuals per taxon demonstrated a number of important issues: (1) some individuals amplify three alleles at one or more loci, suggesting possible aneuploidy or triploidy; (2) some individuals from the same population have identical, or near identical, genotypes which may be indicative of vegetative propagation; (3) genetic profiles among all samples were complex and the microsatellite loci provided moderate support for some of the taxa while other samples suggest a likely history of admixture, hybridisation, or the mis-identification of plant material. These possibilities need to be assessed along with morphological studies being undertaken by Mr Bob Makinson as a complementary component to this research. Particular consideration should be given to the southern populations of the focal taxon which, based on cpDNA and microsatellites, were genetically more similar to the geographically closer G. parviflora subsp. supplicans. Overall, the genetics tools (cpDNA and microsatellites) show great promise for delineating northern populations of G. parviflora subsp. parviflora and for deeper investigations into population genetic structure including the number of unique genotypes within and among populations, the physical spread of clonal genotypes within populations, the genetic differentiation and the genetic health (as assessed by heterozygosity and inbreeding) of populations of this threatened taxon.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by.
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
A chloroplast character separated northern Gpp populations from southern populations and other taxa.
- Taxon
- Grevillea parviflora subsp. parviflora
Grevillea parviflorat0 - Outcome
- consistent charactere0
- Setting
- Hunter areae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One chloroplast region had a consistent character that separated the northern populations of Gpp (northern: Hunter area) from populations in the south of its range (southern: Cumberland Plains area) and all other taxa.”
t0“Grevillea parviflora subsp. parviflora”
Claim · model-written summary
The distinguishing character was an 11 base pair sequence inversion.
- Taxon
- Grevillea parviflora subsp. parvifloraGrevillea parviflorat0
- Outcome
- sequence inversione0
- Value
- 11 base paire0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This character was an 11 base pair sequence inversion.”
t0“Grevillea parviflora subsp. parviflora”
Claim · model-written summary
Nine microsatellite primer pairs consistently amplified variable product in the target taxon.
- Taxon
- Grevillea parviflora subsp. parvifloraGrevillea parviflorat0
- Outcome
- consistently amplified variable producte0
- Value
- nine primer pairse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An assessment of over 50 microsatellite loci led to the identification of nine primer pairs that consistently amplified variable product in the target taxon.”
t0“Grevillea parviflora subsp. parviflora”
Claim · model-written summary
Some individuals amplified three alleles at one or more loci.
- Taxon
- Grevillea parviflora subsp. parvifloraGrevillea parviflorat0
- Outcome
- amplify three allelese0
- Value
- three allelese0
- Qualifier
- suggesting possible aneuploidy or triploidye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some individuals amplify three alleles at one or more loci, suggesting possible aneuploidy or triploidy”
t0“Grevillea parviflora subsp. parviflora”
Claim · model-written summary
Some individuals within the same population had identical or near identical genotypes.
- Taxon
- Grevillea parviflora subsp. parvifloraGrevillea parviflorat0
- Outcome
- identical, or near identical, genotypese0
- Qualifier
- may be indicative of vegetative propagatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some individuals from the same population have identical, or near identical, genotypes which may be indicative of vegetative propagation”
t0“Grevillea parviflora subsp. parviflora”
Claim · model-written summary
Microsatellite profiles moderately supported some taxa.
- Taxon
- Grevillea parviflora subsp. parvifloraGrevillea parviflorat0
- Outcome
- moderate support for some of the taxae0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the microsatellite loci provided moderate support for some of the taxa”
t0“Grevillea parviflora subsp. parviflora”
Claim · model-written summary
Other genetic profiles suggested admixture, hybridisation, or misidentification.
- Taxon
- Grevillea parviflora subsp. parvifloraGrevillea parviflorat0
- Outcome
- likely history of admixture, hybridisation, or the mis-identification of plant materiale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“other samples suggest a likely history of admixture, hybridisation, or the mis-identification of plant material”
t0“Grevillea parviflora subsp. parviflora”
Claim · model-written summary
Southern focal populations were genetically more similar to G. parviflora subsp. supplicans.
- Taxon
- Grevillea parviflora subsp. parvifloraGrevillea parviflorat0
- Outcome
- genetically more similare0
- Direction
- more similare0
- Comparator
- G. parviflora subsp. supplicanse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the southern populations of the focal taxon which, based on cpDNA and microsatellites, were genetically more similar to the geographically closer G. parviflora subsp. supplicans.”
t0“Grevillea parviflora subsp. parviflora”
Claim · model-written summary
The genetic tools showed promise for delineating northern focal populations.
- Taxon
- G. parviflora subsp. parvifloraGrevillea parviflorae0
- Outcome
- delineating northern populationse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the genetics tools (cpDNA and microsatellites) show great promise for delineating northern populations of G. parviflora subsp. parviflora”