Abstract
The allelic variation in high molecular weight glutenins (HMW-Gs) has been reported to account for up to 60% of the variation in bread making quality. In modern cultivars, the HMW-GS allelic variability is not very large and the cultivars used for high quality wheat breeding are very similar in their HMW glutenin composition. Wheat landraces have a higher allelic variability and could be very useful for broadening the currently narrow genetic basis in breeding programs.In this work, the allelic variation of HMW-Gs has been analyzed in a collection of 189 Spanish wheat (Triticum aestivum ssp. vulgare L.) landraces provided by the Plant Genetic Resources Centre. A set of 28 reference cultivars was also included in the analysis. Combining standard electrophoresis techniques (SDS-PAGE) and PCR analysis, we identified five different alleles at the Glu-A1 locus, being the most frequent the b allele (2* subunit). In the Glu-B1 locus, fifteen different subunits combinations were found, the 20x+20y combination, which corresponds to the e allele, was identified in half the accessions. Nine different alleles were identified in the Glu-D1 locus, where the a allele (2+12 subunits) was the most represented. New allelic variation was found for the three loci analysed in the set of landraces. This variation is higher than the obtained in previous studies with landraces, and suggests a high degree of genetic variability in this collection. Afterwards, we tried to link the variability observed for HMW-Gs with the overall genetic diversity of the collection, previously assessed by DArT-seq. Interestingly, accessions with the same Glu-B1 alleles were grouped together in a Principal Coordinates Analysis based on genetic data. The complete description of the HMW glutenin composition of all the landraces analyzed besides further functional quality analyses may favour the use of thesevaluable materials in wheat breeding programs.
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Claims 10
Claim · model-written summary
Five different alleles were identified at the Glu-A1 locus in the landraces.
- Taxon
- wheat
Triticum aestivume7 - Gene
- Glu-A1e0
- Outcome
- different allelese0
- Value
- fivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Spanish wheat (Triticum aestivum ssp. vulgare L.) landraces”
e0“we identified five different alleles at the Glu-A1 locus, being the most frequent the b allele (2* subunit)”
Claim · model-written summary
The b allele (2* subunit) was the most frequent Glu-A1 allele among the landraces.
- Taxon
- wheatTriticum aestivume7
- Gene
- b allele (2* subunit)e0
- Outcome
- most frequente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Spanish wheat (Triticum aestivum ssp. vulgare L.) landraces”
e0“we identified five different alleles at the Glu-A1 locus, being the most frequent the b allele (2* subunit)”
Claim · model-written summary
Fifteen different Glu-B1 subunit combinations were found in the landraces.
- Taxon
- wheatTriticum aestivume7
- Gene
- Glu-B1e1
- Outcome
- subunits combinationse1
- Value
- fifteene1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Spanish wheat (Triticum aestivum ssp. vulgare L.) landraces”
e1“In the Glu-B1 locus, fifteen different subunits combinations were found, the 20x+20y combination, which corresponds to the e allele, was identified in half the accessions”
Claim · model-written summary
The 20x+20y Glu-B1 combination (e allele) was identified in half of the accessions.
- Taxon
- wheatTriticum aestivume7
- Gene
- 20x+20ye1
- Outcome
- identified in half the accessionse1
- Value
- halfe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Spanish wheat (Triticum aestivum ssp. vulgare L.) landraces”
e1“In the Glu-B1 locus, fifteen different subunits combinations were found, the 20x+20y combination, which corresponds to the e allele, was identified in half the accessions”
Claim · model-written summary
Nine different alleles were identified at the Glu-D1 locus in the landraces.
- Taxon
- wheatTriticum aestivume7
- Gene
- Glu-D1e2
- Outcome
- different allelese2
- Value
- Ninee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Spanish wheat (Triticum aestivum ssp. vulgare L.) landraces”
e2“Nine different alleles were identified in the Glu-D1 locus, where the a allele (2+12 subunits) was the most represented”
Claim · model-written summary
The a allele (2+12 subunits) was the most represented Glu-D1 allele.
- Taxon
- wheatTriticum aestivume7
- Gene
- a allele (2+12 subunits)e2
- Outcome
- most representede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Spanish wheat (Triticum aestivum ssp. vulgare L.) landraces”
e2“Nine different alleles were identified in the Glu-D1 locus, where the a allele (2+12 subunits) was the most represented”
Claim · model-written summary
New allelic variation was found at each of the three glutenin loci analysed in the landraces.
- Taxon
- wheatTriticum aestivume7
- Outcome
- New allelic variatione3
- Value
- three locie3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Spanish wheat (Triticum aestivum ssp. vulgare L.) landraces”
e3“New allelic variation was found for the three loci analysed in the set of landraces”
Claim · model-written summary
The HMW glutenin variation found in the landraces is higher than in previous studies with landraces.
- Taxon
- wheatTriticum aestivume7
- Outcome
- This variatione4
- Direction
- highere4
- Comparator
- previous studies with landracese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Spanish wheat (Triticum aestivum ssp. vulgare L.) landraces”
e4“This variation is higher than the obtained in previous studies with landraces, and suggests a high degree of genetic variability in this collection”
Claim · model-written summary
Accessions with the same Glu-B1 alleles grouped together in a principal coordinates analysis of genetic data.
- Taxon
- wheatTriticum aestivume7
- Gene
- Glu-B1 allelese5
- Outcome
- grouped together in a Principal Coordinates Analysise5
- Direction
- grouped togethere5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Spanish wheat (Triticum aestivum ssp. vulgare L.) landraces”
e5“accessions with the same Glu-B1 alleles were grouped together in a Principal Coordinates Analysis based on genetic data”
Claim · model-written summary
The complete HMW glutenin description of the landraces may favour their use in wheat breeding programs.
- Taxon
- wheatTriticum aestivume7
- Outcome
- use of thesevaluable materials in wheat breeding programse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Spanish wheat (Triticum aestivum ssp. vulgare L.) landraces”
e6“may favour the use of thesevaluable materials in wheat breeding programs”