Abstract
Eukaryotic Argonaute proteins play primary roles in mi RNA and si RNA pathways that are essential for numerous developmental and biological processes. However, the functional roles of the four Zm AGO1 genes have not yet been characterized in maize(Zea mays L.). In the present study, Zm AGO1 a was identified from four putative Zm AGO1 genes for further characterization. Complementation of the Arabidopsis ago1-27 mutant with Zm AGO1 a indicated that constitutive overexpression of Zm AGO1 a could restore the smaller rosette, serrated leaves, later flowering and maturation, lower seed set, and darker green leaves at late stages of the mutant to the wild-type phenotype. The expression profiles of Zm AGO1 a under five different abiotic stresses indicated that Zm AGO1 a shares expression patterns similar to those of Argonaute genes in rice, Arabidopsis, and wheat.Further, variation in Zm AGO1 a alleles among diverse maize germplasm that resulted in several amino acid changes revealed genetic diversity at this locus. The present data suggest that Zm AGO1 a might be an important AGO1 ortholog in maize. The results presented provide further insight into the function of ZmAGO1a.
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Claims 5
Claim · model-written summary
Zm AGO1a overexpression restored the smaller rosette, serrated leaves and later flowering and maturation of the Arabidopsis ago1-27 mutant to the wild-type phenotype.
- Material
- ago1-27 mutante1
- Treatment
- constitutive overexpression of Zm AGO1 ae1
- Gene
- Zm AGO1 ae1
- Outcome
- smaller rosette, serrated leaves, later flowering and maturatione1
- Direction
- restoree1
- Comparator
- wild-type phenotypee1
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“characterized in maize(Zea mays L.)”
e1“Complementation of the Arabidopsis ago1-27 mutant with Zm AGO1 a indicated that constitutive overexpression of Zm AGO1 a could restore the smaller rosette, serrated leaves, later flowering and maturation, lower seed set, and darker green leaves at late stages of the mutant to the wild-type phenotype”
Claim · model-written summary
Zm AGO1a overexpression restored the lower seed set and darker green leaves of the ago1-27 mutant to the wild-type phenotype.
- Material
- ago1-27 mutante1
- Treatment
- constitutive overexpression of Zm AGO1 ae1
- Gene
- Zm AGO1 ae1
- Outcome
- seed set, and darker green leavese1
- Direction
- restoree1
- Comparator
- wild-type phenotypee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“characterized in maize(Zea mays L.)”
e1“Complementation of the Arabidopsis ago1-27 mutant with Zm AGO1 a indicated that constitutive overexpression of Zm AGO1 a could restore the smaller rosette, serrated leaves, later flowering and maturation, lower seed set, and darker green leaves at late stages of the mutant to the wild-type phenotype”
Claim · model-written summary
Zm AGO1a expression under five abiotic stresses was similar to that of Argonaute genes in rice, Arabidopsis and wheat.
- Treatment
- five different abiotic stressese2
- Gene
- Zm AGO1 ae2
- Outcome
- expression profilese2
- Direction
- similare2
- Comparator
- Argonaute genes in rice, Arabidopsis, and wheate2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“characterized in maize(Zea mays L.)”
e2“The expression profiles of Zm AGO1 a under five different abiotic stresses indicated that Zm AGO1 a shares expression patterns similar to those of Argonaute genes in rice, Arabidopsis, and wheat”
Claim · model-written summary
Variation in Zm AGO1a alleles among diverse maize germplasm revealed genetic diversity at this locus.
- Material
- diverse maize germplasme3
- Gene
- Zm AGO1 a allelese3
- Outcome
- genetic diversitye3
- Qualifier
- several amino acid changese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“characterized in maize(Zea mays L.)”
e3“variation in Zm AGO1 a alleles among diverse maize germplasm that resulted in several amino acid changes revealed genetic diversity at this locus”
Claim · model-written summary
Zm AGO1a was identified from four putative Zm AGO1 genes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“characterized in maize(Zea mays L.)”
e0“Zm AGO1 a was identified from four putative Zm AGO1 genes for further characterization”