Abstract
【Objective】Detecting various types of citrus viroids by RT-PCR and characterizing the subtypes by sequencing in Hunan Province, China【.Method】Total RNA was isolated from phloem of young shoots and retro-transcripted to cDNA by RT-PCR with specific primers, and the amplified cDNA fragments were sequenced for identification of subtypes. 【Result】An effective RT-PCR technique was established for detecting citrus viroid types, and three types were recognized, i.e. CEVd, CVdⅡ and CVdⅢ, and the later two were identified for the first time in China. The sequencing results indicated that the CVdⅡ seemed to be a new subtype; CVdⅢb detected in the present study could be further developed as a dwarfing agent. 【Conclusion】Three types of citrus viroids were detected, amongst, CVdⅡ and CVdⅢ were identified for the first time in China. The sequence of CVdⅡ was different from all of the three sub-types reported, implying the identification of a new subtype. The verified CVdⅢb offered a possibility of using it in dwarfing cultivation.
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Claims 6
Claim · model-written summary
An effective RT-PCR technique for citrus viroid detection was established.
- Outcome
- detecting citrus viroid typese0
CitrusNot reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An effective RT-PCR technique was established for detecting citrus viroid types”
t0“Citrus”
Claim · model-written summary
Three citrus viroid types were recognized: CEVd, CVdⅡ, and CVdⅢ.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three types were recognized, i.e. CEVd, CVdⅡ and CVdⅢ”
t0“Citrus Viroids”
Claim · model-written summary
CVdⅡ was identified for the first time in China.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CVdⅡ and CVdⅢ were identified for the first time in China”
t0“Citrus”
Claim · model-written summary
CVdⅢ was identified for the first time in China.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CVdⅡ and CVdⅢ were identified for the first time in China”
t0“Citrus”
Claim · model-written summary
CVdⅡ sequence differed from three reported subtypes, suggesting a new subtype.
- Interactor
- CVdⅡe0pathogen
- Outcome
- sequence of CVdⅡe0
- Direction
- differente0
- Comparator
- three sub-types reportede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sequence of CVdⅡ was different from all of the three sub-types reported, implying the identification of a new subtype”
t0“Citrus”
Claim · model-written summary
Verified CVdⅢb was proposed as a possible dwarfing agent.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The verified CVdⅢb offered a possibility of using it in dwarfing cultivation”
t0“Citrus”