Paper · 2021 · African Journal of Health Safety and Environment

GENETIC DIVERSITY OF Digitaria horizontalis Willd. IN BUILT ENVIRONMENTS IN BENIN CITY, NIGERIA

B. Ikhajiagbe; V. D. Odenore; E. O. Umeokeke; et al.

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claims
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verbatim quotes
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citations
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Open access · cc-by

Abstract

The influence of humans on biodiversity at the genetic, species, community, and ecosystem levels is enormous. This is even more impactful on plant populations, which, being immobile, are at the mercy of their immediate environment. Digitaria horizontalis is a very common grass species in Benin City. Its ruderal nature accounts mostly for its widespread. The current study investigated the existence of possible genetic diversity of the grass in built environment in Benin City. Plant samples were randomly collected from 21 built environment locations within 6 Local Government Council Areas that make up Benin City. Test plants were allowed to acclimatize in experimental bowls and then allowed to grow for 4 weeks after which morphological characteristics were determined. Genetic diversity was determined by Random Amplification of Polymorphic DNA. Significant differences in plant height (33.0 – 59.0 cm) occurred when plants were compared based on location of collection. Changes in patterns of the association with weeds within the vicinity of the test plant was observed. The most prevalent plant species associated with the test plant was Pennisetum purpureum with a 12.18% prevalence. RAPD analysis using primer OPA 04 revealed a locus (about 600bp) that was absent in one or more of the locations with minimum disturbance. This 600pb fragment was however observed in most samples from highly built up and trampled environments. Primer OPA 03 showed monomorphic bands in 2 test samples with a band size of 400bp. It is therefore reported that there is an existence of the genetic diversity in a landscape of grass species (Digitaria horizontalis) found in different built environment in Benin City, Edo State, Nigeria.

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Claims 7

Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Plant height differed significantly among collection locations, ranging from 33.0 to 59.0 cm.

Taxon
Digitaria horizontalisDigitaria horizontalise1
Outcome
plant heighte0
Direction
differencese0
Value
33.0 – 59.0 cme0
Setting
location of collectione0

Verbatim

e0

“Significant differences in plant height (33.0 – 59.0 cm) occurred when plants were compared based on location of collection.”

✓ located verbatim in the source · abstract, characters 854–978 · show in text ↑
e1

“Digitaria horizontalis is a very common grass species in Benin City.”

✓ located verbatim in the source · abstract, characters 232–300 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The pattern of weed associations near Digitaria horizontalis changed.

Taxon
Digitaria horizontalisDigitaria horizontalise1
Outcome
patterns of the association with weedse0

Verbatim

e0

“Changes in patterns of the association with weeds within the vicinity of the test plant was observed.”

✓ located verbatim in the source · abstract, characters 979–1080 · show in text ↑
e1

“Digitaria horizontalis is a very common grass species in Benin City.”

✓ located verbatim in the source · abstract, characters 232–300 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Pennisetum purpureum was the most prevalent associated species, at 12.18%.

Taxon
Digitaria horizontalisDigitaria horizontalise1
Interactor
Pennisetum purpureumCenchrus purpureuse0other
Outcome
prevalencee0
Direction
most prevalente0
Value
12.18%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cenchrus purpureus
Cenchrus purpureus · InteractorForest and Kim Starr · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The most prevalent plant species associated with the test plant was Pennisetum purpureum with a 12.18% prevalence.”

✓ located verbatim in the source · abstract, characters 1081–1195 · show in text ↑
e1

“Digitaria horizontalis is a very common grass species in Benin City.”

✓ located verbatim in the source · abstract, characters 232–300 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

An approximately 600bp OPA 04 locus was absent from one or more minimally disturbed locations.

Taxon
Digitaria horizontalisDigitaria horizontalise1
Gene
a locuse0
Outcome
absente0
Value
about 600bpe0
Setting
locations with minimum disturbancee0

Verbatim

e0

“RAPD analysis using primer OPA 04 revealed a locus (about 600bp) that was absent in one or more of the locations with minimum disturbance.”

✓ located verbatim in the source · abstract, characters 1196–1334 · show in text ↑
e1

“Digitaria horizontalis is a very common grass species in Benin City.”

✓ located verbatim in the source · abstract, characters 232–300 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The 600pb fragment appeared in most samples from heavily built and trampled environments.

Taxon
Digitaria horizontalisDigitaria horizontalise1
Outcome
fragment was however observede0
Value
600pbe0
Setting
highly built up and trampled environmentse0

Verbatim

e0

“This 600pb fragment was however observed in most samples from highly built up and trampled environments.”

✓ located verbatim in the source · abstract, characters 1335–1439 · show in text ↑
e1

“Digitaria horizontalis is a very common grass species in Benin City.”

✓ located verbatim in the source · abstract, characters 232–300 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

OPA 03 showed 400bp monomorphic bands in two samples.

Taxon
Digitaria horizontalisDigitaria horizontalise1
Outcome
monomorphic bandse0
Value
400bpe0
Qualifier
2 test samplese0

Verbatim

e0

“Primer OPA 03 showed monomorphic bands in 2 test samples with a band size of 400bp.”

✓ located verbatim in the source · abstract, characters 1440–1523 · show in text ↑
e1

“Digitaria horizontalis is a very common grass species in Benin City.”

✓ located verbatim in the source · abstract, characters 232–300 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Digitaria horizontalis shows genetic diversity across built environments of Benin City.

Taxon
Digitaria horizontalisDigitaria horizontalise0
Outcome
genetic diversitye0
Setting
Benin City, Edo State, Nigeriae0

Verbatim

e0

“there is an existence of the genetic diversity in a landscape of grass species (Digitaria horizontalis) found in different built environment in Benin City, Edo State, Nigeria.”

✓ located verbatim in the source · abstract, characters 1554–1729 · show in text ↑