Abstract
Phytophthora infestans causes late blight disease on potato and tomato and is currently controlled by resistant cultivars or intensive fungicide spraying. Here, we investigated an alternative means for late blight control by spraying potato leaves with double-stranded RNAs (dsRNA) that target the P. infestans genes essential for infection. First, we showed that the sporangia of P. infestans expressing green fluorescent protein (GFP) can take up in vitro synthesized dsRNAs homologous to GFP directly from their surroundings, including leaves, which led to the reduced relative expression of GFP. We further demonstrate the potential of spray-induced gene silencing (SIGS) in controlling potato late blight disease by targeting developmentally important genes in P. infestans such as guanine-nucleotide binding protein β-subunit (PiGPB1), haustorial membrane protein (PiHmp1), cutinase (PiCut3), and endo-1,3(4)-β-glucanase (PiEndo3). Our results demonstrate that SIGS can potentially be used to mitigate potato late blight; however, the degree of disease control is dependent on the selection of the target genes. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
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Claims 3
Claim · model-written summary
P. infestans sporangia took up GFP homologous dsRNAs from their surroundings, including leaves.
- Taxon
- potato
Solanum tuberosumt0 - Interactor
- P. infestans
Phytophthora infestanse0pathogen - Outcome
- sporangia of P. infestans expressing green fluorescent protein (GFP) can take up in vitro synthesized dsRNAs homologous to GFPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the sporangia of P. infestans expressing green fluorescent protein (GFP) can take up in vitro synthesized dsRNAs homologous to GFP directly from their surroundings, including leaves”
t0“Potato”
Claim · model-written summary
Uptake of GFP homologous dsRNA reduced relative GFP expression in P. infestans.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- in vitro synthesized dsRNAs homologous to GFPe0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- GFPe0
- Outcome
- relative expression of GFPe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“First, we showed that the sporangia of P. infestans expressing green fluorescent protein (GFP) can take up in vitro synthesized dsRNAs homologous to GFP directly from their surroundings, including leaves, which led to the reduced relative expression of GFP.”
t0“Potato”
Claim · model-written summary
SIGS targeting P. infestans developmental genes potentially mitigated potato late blight, with control dependent on gene choice.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- SIGSe0
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Outcome
- potato late blighte0
- Direction
- mitigatee0
- Qualifier
- dependent on the selection of the target genese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results demonstrate that SIGS can potentially be used to mitigate potato late blight; however, the degree of disease control is dependent on the selection of the target genes.”
e1“P. infestans genes essential for infection”
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