Abstract
In the present study, the effect of a commercial extract of the seaweed Ascophyllum nodosum on in vitro micropropagation, shoot regeneration, and rhizoghenesis were studied in Nicotiana benthamiana and Prunus domestica. Results showed that the MS medium supplemented with various concentrations of the Ascophyllum extract (5, 10, 50, and 100 mg L−1) significantly enhanced the number of regenerated buds from N. benthamiana leaf discs to the conventional MS regenerating medium. Increases ranged from 3.5 to 6.5 times higher than the control. The effect of the Ascophyllum extract on N. benthamiana micropropagation was assessed through the measurement of some plant growth parameters. Results showed that the extract alone could not replace the micropropagation medium since shoot length, shoot diameter, root length, and leaf area were significantly reduced. However, its combination with a half-strength MS medium enhanced these parameters. Its effect was also evaluated on regeneration from plum hypocotyl slices. When added to the shoot regeneration medium without any plant growth regulators, the Ascophyllum extract alone could induce shoot regeneration. However, the percentage of bud regeneration and number of regenerated buds were lower than with the conventional shoot regeneration medium containing complete growth regulators. In contrast, the Ascophyllum extract drastically promoted rhizogenesis from plum hypocotyl slices. These results pave the way for the possible use of A. nodosum extracts in in vitro mass propagation of higher plants.
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Claims 6
Claim · model-written summary
Ascophyllum extract increased regenerated buds from tobacco leaf discs by 3.5 to 6.5 times.
- Taxon
- N. benthamiana
Nicotiana benthamianae0 - Organ / stage
- leaf discse0
- Treatment
- Ascophyllum extract (5, 10, 50, and 100 mg L−1)e0
- Outcome
- number of regenerated budse0
- Direction
- enhancede0
- Value
- 3.5 to 6.5 timese0
- Comparator
- conventional MS regenerating mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with various concentrations of the Ascophyllum extract (5, 10, 50, and 100 mg L−1) significantly enhanced the number of regenerated buds from N. benthamiana leaf discs to the conventional MS regenerating medium. Increases ranged from 3.5 to 6.5 times higher than the control”
Claim · model-written summary
Extract alone reduced tobacco shoot and root lengths, shoot diameter and leaf area versus micropropagation medium.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Treatment
- extract alonee0
- Outcome
- shoot length, shoot diameter, root length, and leaf areae0
- Direction
- reducede0
- Comparator
- micropropagation mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extract alone could not replace the micropropagation medium since shoot length, shoot diameter, root length, and leaf area were significantly reduced”
t0“Nicotiana benthamiana”
e1“N. benthamiana leaf discs”
Claim · model-written summary
Extract with half-strength MS medium enhanced those tobacco growth parameters.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- combination with a half-strength MS mediume0
- Outcome
- shoot length, shoot diameter, root length, and leaf areae1
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its combination with a half-strength MS medium enhanced these parameters”
t0“Nicotiana benthamiana”
e1“shoot length, shoot diameter, root length, and leaf area were significantly reduced”
Claim · model-written summary
Ascophyllum extract alone induced shoot regeneration in plum hypocotyl slices without growth regulators.
- Taxon
- Prunus domestica
Prunus domesticat0 - Organ / stage
- hypocotyl slicese1
- Treatment
- Ascophyllum extract alonee0
- Outcome
- shoot regeneratione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When added to the shoot regeneration medium without any plant growth regulators, the Ascophyllum extract alone could induce shoot regeneration”
t0“Prunus domestica”
e1“regeneration from plum hypocotyl slices”
Claim · model-written summary
Plum bud regeneration percentage and bud number were lower with extract alone than conventional medium.
- Taxon
- Prunus domesticaPrunus domesticat0
- Treatment
- Ascophyllum extracte1
- Outcome
- percentage of bud regeneration and number of regenerated budse0
- Direction
- lowere0
- Comparator
- conventional shoot regeneration medium containing complete growth regulatorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the percentage of bud regeneration and number of regenerated buds were lower than with the conventional shoot regeneration medium containing complete growth regulators”
t0“Ascophyllum nodosum-Based Soluble Extract on Micropropagation and Regeneration of Nicotiana benthamiana and Prunus domestica”
e1“the Ascophyllum extract alone could induce shoot regeneration”
Claim · model-written summary
Ascophyllum extract promoted rhizogenesis from plum hypocotyl slices.
- Taxon
- plumPrunus domesticae0
- Organ / stage
- hypocotyl slicese0
- Treatment
- Ascophyllum extracte0
- Outcome
- rhizogenesise0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Ascophyllum extract drastically promoted rhizogenesis from plum hypocotyl slices”
The same questions in other papers
botanical · growth · Prunus domestica (plum)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.