Abstract
<ns2:p>CRISPR/Cas9 system with deactivated or dead nuclease domain of Cas9 (known as dCas9) can also be used as a DNA binding protein to target specific DNA sequences. Similar to zinc fingers and transcription activator like effectors (TALEs), dCas9 can be fused with other effector domains for different purposes such as gene activation, gene repression, modification of epigenetic marks etc. Previously, TALEs were used to target and occupy the most conserved nonanucleotide sequence of Cotton Leaf Curl Virus (CLCuV) with promising results. The purpose of this study is to check the efficiency of dCas9 as DNA binding protein targeting the nonanucleotide sequence of CLCuV to inhibit virus replication. Nicotiana benthamiana was used to evaluate the efficiency of CRISPR/dCas9 system for viral interference. The resistance against virus infection and suppression of replication of the virus was assessed by PCR, RT-PCR and symptoms development on plant leaves in terms of days’ post inoculation (dpi). Expression of the Cas9 and gRNA was quantified using RT-PCR. The results showed partial inhibition of CLCuV replication, lower disease symptoms and virus accumulation as compared to control plants. A comparison of current and previous results using TALEs showed that dCas9 was slightly less efficient to suppress virus replication. Multiplexing of gene editing techniques could be the way forward to engineer virus resistance in plants.</ns2:p>
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Claims 5
Claim · model-written summary
CRISPR/dCas9 partially inhibited CLCuV replication in Nicotiana benthamiana compared with control plants.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae3 - Treatment
- CRISPR/dCas9 systeme3
- Interactor
- CLCuVCotton leaf curl viruse1pathogen
- Outcome
- CLCuV replicatione1
- Direction
- partial inhibitione1
- Comparator
- control plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The results showed partial inhibition of CLCuV replication, lower disease symptoms and virus accumulation as compared to control plants”
e3“Nicotiana benthamiana was used to evaluate the efficiency of CRISPR/dCas9 system for viral interference”
Claim · model-written summary
CRISPR/dCas9 lowered disease symptoms in Nicotiana benthamiana compared with control plants.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae3
- Treatment
- CRISPR/dCas9 systeme3
- Interactor
- CLCuVCotton leaf curl viruse1pathogen
- Outcome
- disease symptomse1
- Direction
- lowere1
- Comparator
- control plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The results showed partial inhibition of CLCuV replication, lower disease symptoms and virus accumulation as compared to control plants”
e3“Nicotiana benthamiana was used to evaluate the efficiency of CRISPR/dCas9 system for viral interference”
Claim · model-written summary
CRISPR/dCas9 lowered virus accumulation in Nicotiana benthamiana compared with control plants.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae3
- Treatment
- CRISPR/dCas9 systeme3
- Interactor
- CLCuVCotton leaf curl viruse1pathogen
- Outcome
- virus accumulatione1
- Direction
- lowere1
- Comparator
- control plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The results showed partial inhibition of CLCuV replication, lower disease symptoms and virus accumulation as compared to control plants”
e3“Nicotiana benthamiana was used to evaluate the efficiency of CRISPR/dCas9 system for viral interference”
Claim · model-written summary
dCas9 was slightly less efficient than TALEs in suppressing virus replication in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae3
- Treatment
- dCas9e4
- Outcome
- virus replicatione4
- Direction
- less efficiente4
- Qualifier
- slightlye4
- Comparator
- TALEse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Nicotiana benthamiana was used to evaluate the efficiency of CRISPR/dCas9 system for viral interference”
e4“A comparison of current and previous results using TALEs showed that dCas9 was slightly less efficient to suppress virus replication”
Claim · model-written summary
Multiplexing of gene editing techniques could be the way forward to engineer virus resistance in plants.
- Taxon
- plantsViridiplantaee5
- Outcome
- virus resistancee5
Verbatim
e5“Multiplexing of gene editing techniques could be the way forward to engineer virus resistance in plants”
The same questions in other papers
loss of function · Cotton leaf curl virus · Nicotiana benthamianaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.