Abstract
Curcuma longa (C. longa) is widely known for its medicinal properties. However, the potential overexploitation of this plant raises doubts about its long-term survival on Rapa Nui. Micropropagation using a temporary immersion system (TIS) could be the basis for developing a cost-effective and highly productive method of large-scale cultivation of this plant. Our objective was to develop and refine the in vitro multiplication system for mass propagation of C. longa, and thus help restore the fragile ecosystem of Rapa Nui. Three parameters were evaluated: number of explants per flask, flask capacity, and LEDs spectrum. For each parameter evaluated, four aspects were analyzed: fresh weight per plant, number of shoots, percentage of non-sprouting explants, and the proliferation rate. The use of 30 explants per two-liter flask results in more plants with high fresh biomass than other configurations. In addition, LEDs with a red:blue ratio of 2:1 provided the best lighting conditions for in vitro propagation and positively affected C. longa proliferation and rooting. Therefore, our results show that 30 explants per two-liter flask and an LED source with a red:blue ratio of 2:1 allow a higher number of C. longa plants to be obtained using TIS.
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Claims 5
Claim · model-written summary
Thirty explants per two-liter flask produced more plants with high fresh biomass than other configurations.
- Treatment
- 30 explants per two-liter flaske0
- Outcome
- plants with high fresh biomasse0
- Direction
- moree0
- Comparator
- other configurationse0
Not reportedTaxon
Verbatim
e0“The use of 30 explants per two-liter flask results in more plants with high fresh biomass than other configurations”
Claim · model-written summary
A red:blue LED ratio of 2:1 gave the best lighting conditions for in vitro propagation.
Not reportedTaxonComparator
Verbatim
e0“LEDs with a red:blue ratio of 2:1 provided the best lighting conditions for in vitro propagation”
Claim · model-written summary
A red:blue LED ratio of 2:1 positively affected C. longa proliferation.
- Taxon
- C. longa
_W_IMG_2440.jpg?width=60)
Curcuma longae0 - Treatment
- LEDs with a red:blue ratio of 2:1e0
- Outcome
- C. longa proliferatione0
- Direction
- positively affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_W_IMG_2440.jpg?width=500)
Verbatim
e0“LEDs with a red:blue ratio of 2:1 provided the best lighting conditions for in vitro propagation and positively affected C. longa proliferation and rooting”
Claim · model-written summary
A red:blue LED ratio of 2:1 positively affected C. longa rooting.
- Taxon
- C. longaCurcuma longae0
- Treatment
- LEDs with a red:blue ratio of 2:1e0
- Outcome
- rootinge0
- Direction
- positively affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LEDs with a red:blue ratio of 2:1 provided the best lighting conditions for in vitro propagation and positively affected C. longa proliferation and rooting”
Claim · model-written summary
Thirty explants per two-liter flask with red:blue LED light at 2:1 yielded a higher number of C. longa plants using TIS.
- Taxon
- C. longaCurcuma longae0
- Treatment
- 30 explants per two-liter flask and an LED source with a red:blue ratio of 2:1e0
- Outcome
- number of C. longa plantse0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“30 explants per two-liter flask and an LED source with a red:blue ratio of 2:1 allow a higher number of C. longa plants to be obtained using TIS”
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