Abstract
Plant non-specific lipid transfer proteins (nsLTPs) can transfer lipids in vitro, regulate plant growth and development, and respond to environmental abiotic and biotic stresses. In this study, 74 nsLTPs genes were identified from the genome of Nicotiana tabacum variety K326, and we analyzed multiple characteristics of these genes, including phylogeny, gene structures, conserved motifs, protein domains, chromosome locations, cis-elements in the promoter sequences, 3D structure, and the expression patterns under different hormones and abiotic stresses. The results revealed that nsLTPs in tobacco could be divided into eight types, including type I, II, III, IV, V, VII, VIII, and XIII, according to the interval and sequence similarity between the eight cysteines. The same types of NtLTPs had similar intron-exon patterns and conserved motifs, motif 2 and motif 3 were the characteristic motifs of NtLTPs family. In the process of evolution, fragment duplication dominated the expansion of the NtLTPs family. RNA-seq analysis after drought treatment revealed that the functional differentiation patterns of repeat gene pairs were diverse during evolution period. Promoter analysis showed that they contained a variety of cis-acting elements in response to light response, hormones, and abiotic stress. Furthermore, qRT-PCR demonstrated that NtLTPs family genes had different expression patterns in different tissues and organs of tobacco plants, which could respond to abiotic stresses such as drought, salt, and hormone treatments (IAA, GA, and SA etc.). These results provide a theoretical reference for the in-depth analysis of the functions of NtLTPs family genes and molecular breeding.
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Claims 9
Claim · model-written summary
The K326 tobacco genome contains 74 identified nsLTP genes.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume0 - Material
- K326e0
- Gene
- nsLTPse0
- Outcome
- 74 nsLTPs genese0
- Value
- 74e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“74 nsLTPs genes were identified from the genome of Nicotiana tabacum variety K326”
Claim · model-written summary
Tobacco nsLTPs fall into eight sequence-based types.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- nsLTPse0
- Outcome
- eight types, including type I, II, III, IV, V, VII, VIII, and XIIIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nsLTPs in tobacco could be divided into eight types, including type I, II, III, IV, V, VII, VIII, and XIII, according to the interval and sequence similarity between the eight cysteines”
Claim · model-written summary
NtLTPs of the same type have similar intron-exon patterns and conserved motifs.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- NtLTPse0
- Outcome
- similar intron-exon patterns and conserved motifse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The same types of NtLTPs had similar intron-exon patterns and conserved motifs”
t0“tobacco”
Claim · model-written summary
Motifs 2 and 3 characterize the NtLTP family.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- NtLTPse0
- Outcome
- motif 2 and motif 3 were the characteristic motifse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“motif 2 and motif 3 were the characteristic motifs of NtLTPs family”
t0“tobacco”
Claim · model-written summary
Fragment duplication dominated expansion of the NtLTP family.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- NtLTPse0
- Outcome
- fragment duplication dominated the expansione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fragment duplication dominated the expansion of the NtLTPs family”
t0“tobacco”
Claim · model-written summary
Repeated gene pairs displayed diverse functional differentiation patterns after drought treatment.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- drought treatmente0
- Outcome
- functional differentiation patterns of repeat gene pairs were diversee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq analysis after drought treatment revealed that the functional differentiation patterns of repeat gene pairs were diverse during evolution period”
t0“tobacco”
Claim · model-written summary
NtLTP promoters contain cis-acting elements responsive to light, hormones and abiotic stress.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- a variety of cis-acting elements in response to light response, hormones, and abiotic stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promoter analysis showed that they contained a variety of cis-acting elements in response to light response, hormones, and abiotic stress”
t0“tobacco”
Claim · model-written summary
NtLTP genes have differing expression patterns among tobacco tissues and organs.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- NtLTPse0
- Outcome
- different expression patterns in different tissues and organse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtLTPs family genes had different expression patterns in different tissues and organs of tobacco plants”
Claim · model-written summary
NtLTP genes responded to drought, salt and hormone treatments.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- drought, salt, and hormone treatments (IAA, GA, and SA etc.)e0
- Outcome
- respond to abiotic stressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which could respond to abiotic stresses such as drought, salt, and hormone treatments (IAA, GA, and SA etc.)”
t0“tobacco”