Abstract
Gerbera jamesonii Bolus ex Hooker f. (African daisy) is listed among the top five most important ornamental plants in the global floricultural industry. To satisfy its demand, the floriculture industry relies on reproducible and effective propagation protocol while retaining the genetic uniformity of G. jamesonii . The present study, for the first time, reports the potential of picloram for enhanced induction of organogenic calli from leaves of G. jamesonii and its high-frequency indirect regeneration. The fastest induction of calli with maximum fresh and dry weight was recorded in the Murashige and Skoog (MS) semisolid medium supplemented with 1 mg/l picloram. In addition, callus induction was observed in 2,4-dichlorophenoxy acetic acid- and α-napthaleneaceticacid-supplemented media but with delayed response and reduced fresh and dry weight. The proliferated calli were transferred to shoot induction media containing MS salt and 0.5–1 mg/l N 6 -benzylaminopurine, kinetin, or thidiazuron. A mean number of ~6 shoots per callus were developed after 5 days of culture in the MS medium supplemented with 1 mg/l kinetin, with a mean length of 5.2 cm. Successful rooting of shoots was achieved in the MS medium fortified with 1.5 mg/l indole-3-acetic acid, wherein the earliest root initiation (~5 days), as well as the maximum number (~9) and length (~4.8 cm) of roots, were recorded. Complete plantlets were primarily acclimatized in sand before being transferred to a mixed substrate (of soil, sand, tea leaf waste, and cow urine) that secured >90% survival and further growth of the plantlets. Eventually, clonal fidelity of the in vitro regenerants assessed via inter-simple sequence repeats (ISSR) primers exhibited a monomorphic banding patterns that suggested genetic integrity within the plantlets as well as with their mother plant. The results of the present study should be of interest for commercial propagation and mutagenesis- as well as genetic transformation-related research.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
One mg/l picloram in MS medium produced the fastest callus induction and maximum fresh and dry weight.
- Taxon
- G. jamesonii
Gerbera jamesoniie1 - Treatment
- 1 mg/l piclorame0
- Outcome
- induction of callie0
- Direction
- fasteste0
- Value
- 1 mg/le0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fastest induction of calli with maximum fresh and dry weight was recorded in the Murashige and Skoog (MS) semisolid medium supplemented with 1 mg/l picloram.”
e1“of G. jamesonii and its high-frequency indirect regeneration”
Claim · model-written summary
2,4-dichlorophenoxy acetic acid medium induced callus with delayed response and reduced weight.
- Taxon
- G. jamesoniiGerbera jamesoniie1
- Treatment
- 2,4-dichlorophenoxy acetic acide0
- Outcome
- callus inductione0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus induction was observed in 2,4-dichlorophenoxy acetic acid- and α-napthaleneaceticacid-supplemented media but with delayed response and reduced fresh and dry weight”
e1“of G. jamesonii and its high-frequency indirect regeneration”
Claim · model-written summary
α-napthaleneaceticacid medium induced callus with delayed response and reduced weight.
- Taxon
- G. jamesoniiGerbera jamesoniie1
- Treatment
- α-napthaleneaceticacide0
- Outcome
- callus inductione0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus induction was observed in 2,4-dichlorophenoxy acetic acid- and α-napthaleneaceticacid-supplemented media but with delayed response and reduced fresh and dry weight”
e1“of G. jamesonii and its high-frequency indirect regeneration”
Claim · model-written summary
MS medium with 1 mg/l kinetin produced about six shoots per callus after five days.
- Taxon
- G. jamesoniiGerbera jamesoniie1
- Treatment
- 1 mg/l kinetine0
- Outcome
- shoots per calluse0
- Direction
- developede0
- Value
- ~6e0
- Qualifier
- 5.2 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A mean number of ~6 shoots per callus were developed after 5 days of culture in the MS medium supplemented with 1 mg/l kinetin, with a mean length of 5.2 cm.”
e1“of G. jamesonii and its high-frequency indirect regeneration”
Claim · model-written summary
MS medium with 1.5 mg/l indole-3-acetic acid produced earliest root initiation in about five days.
- Taxon
- G. jamesoniiGerbera jamesoniie1
- Treatment
- 1.5 mg/l indole-3-acetic acide0
- Outcome
- root initiatione0
- Direction
- earlieste0
- Value
- ~5 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Successful rooting of shoots was achieved in the MS medium fortified with 1.5 mg/l indole-3-acetic acid, wherein the earliest root initiation (~5 days), as well as the maximum number (~9) and length (~4.8 cm) of roots, were recorded.”
e1“of G. jamesonii and its high-frequency indirect regeneration”
Claim · model-written summary
MS medium with 1.5 mg/l indole-3-acetic acid produced about nine roots.
- Taxon
- G. jamesoniiGerbera jamesoniie1
- Treatment
- 1.5 mg/l indole-3-acetic acide0
- Outcome
- rootse0
- Direction
- maximume0
- Value
- ~9e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Successful rooting of shoots was achieved in the MS medium fortified with 1.5 mg/l indole-3-acetic acid, wherein the earliest root initiation (~5 days), as well as the maximum number (~9) and length (~4.8 cm) of roots, were recorded.”
e1“of G. jamesonii and its high-frequency indirect regeneration”
Claim · model-written summary
MS medium with 1.5 mg/l indole-3-acetic acid produced roots about 4.8 cm long.
- Taxon
- G. jamesoniiGerbera jamesoniie1
- Treatment
- 1.5 mg/l indole-3-acetic acide0
- Outcome
- lengthe0
- Direction
- maximume0
- Value
- ~4.8 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Successful rooting of shoots was achieved in the MS medium fortified with 1.5 mg/l indole-3-acetic acid, wherein the earliest root initiation (~5 days), as well as the maximum number (~9) and length (~4.8 cm) of roots, were recorded.”
e1“of G. jamesonii and its high-frequency indirect regeneration”
Claim · model-written summary
Sand acclimatization followed by a mixed substrate gave plantlets over 90% survival.
- Taxon
- G. jamesoniiGerbera jamesoniie1
- Treatment
- mixed substratee0
- Outcome
- survivale0
- Direction
- securede0
- Value
- >90%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Complete plantlets were primarily acclimatized in sand before being transferred to a mixed substrate (of soil, sand, tea leaf waste, and cow urine) that secured >90% survival and further growth of the plantlets.”
e1“of G. jamesonii and its high-frequency indirect regeneration”
Claim · model-written summary
ISSR banding patterns indicated genetic integrity of regenerants and their mother plant.
- Taxon
- G. jamesoniiGerbera jamesoniie1
- Outcome
- genetic integritye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“clonal fidelity of the in vitro regenerants assessed via inter-simple sequence repeats (ISSR) primers exhibited a monomorphic banding patterns that suggested genetic integrity within the plantlets as well as with their mother plant”
e1“of G. jamesonii and its high-frequency indirect regeneration”
The same questions in other papers
kinetin · growth · Gerbera jamesonii (Transvaal daisy)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
indole-3-acetic acid · growth · Gerbera jamesonii (Transvaal daisy)Results agree
7 papers in the same direction as this paper
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →growing medium · survival · Gerbera jamesonii (Transvaal daisy)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.