Abstract
Plant cytochrome P450 (P450) participates in a wide range of biosynthetic reactions and targets a variety of biological molecules. These reactions lead to various fatty acid conjugates, plant hormones, secondary metabolites, lignin, and various defensive compounds. In our previous research, transcriptome analysis was performed on the salt-tolerant upland cotton “Tongyan No. 1.” Many differentially expressed genes (DEGs) belong to the P450 family, and their domains occur widely in plants. In this current research, P450 genes were identified in Gossypium hirsutum with the aid of bioinformatics methods for investigating phylogenetic relations, gene structure, cis- elements, chromosomal localization, and collinearity within a genome. qRT-PCR was conducted to analyze P450 gene expression patterns under salt stress. The molecular weights of the 156 P450 genes were in the range of 5,949.6–245,576.3 Da, and the length of the encoded amino acids for all the identified P450 genes ranged from 51 to 2,144. P450 proteins are divided into four different subfamilies based on phylogenetic relationship, gene structure, and chromosomal localization of gene replication. The length of P450 genes in upland cotton differs greatly, ranging from 1,500 to 13,000 bp. The number of exons in the P450 family genes ranged from 1 to 9, while the number of introns ranged from 0 to 8, and there were similar trends within clusters. A total of 31 cis- acting elements were identified by analyzing 1,500 bp promoter sequences. Differences were found in cis- acting elements among genes. The consistency between qRT-PCR and previous transcriptome analysis of salt tolerance DEGs indicated that they were likely to be involved in the salt tolerance of cotton seedlings. Our results provide valuable information on the evolutionary relationships of genes and functional characteristics of the gene family, which is beneficial for further study of the cotton P450 gene family.
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Claims 9
Claim · model-written summary
The 156 P450 genes encoded proteins of molecular weights 5,949.6–245,576.3 Da.
- Taxon
- Gossypium hirsutum
Gossypium hirsutumt0 - Gene
- P450 genese0
- Outcome
- molecular weightse0
- Value
- 5,949.6–245,576.3 Dae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The molecular weights of the 156 P450 genes were in the range of 5,949.6–245,576.3 Da”
t0“Gossypium hirsutum”
Claim · model-written summary
Encoded amino acid lengths ranged from 51 to 2,144.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- P450 genese0
- Outcome
- length of the encoded amino acidse0
- Value
- 51 to 2,144e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the length of the encoded amino acids for all the identified P450 genes ranged from 51 to 2,144.”
t0“Gossypium hirsutum”
Claim · model-written summary
P450 proteins grouped into four subfamilies.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- P450 proteinse0
- Outcome
- four different subfamiliese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P450 proteins are divided into four different subfamilies based on phylogenetic relationship, gene structure, and chromosomal localization of gene replication.”
t0“Gossypium hirsutum”
Claim · model-written summary
Cotton P450 genes ranged from 1,500 to 13,000 bp.
Verbatim
e0“The length of P450 genes in upland cotton differs greatly, ranging from 1,500 to 13,000 bp.”
Claim · model-written summary
P450 exon counts ranged from 1 to 9.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- P450 family genese0
- Outcome
- number of exonse0
- Value
- 1 to 9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of exons in the P450 family genes ranged from 1 to 9”
t0“Gossypium hirsutum”
Claim · model-written summary
P450 intron counts ranged from 0 to 8.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Outcome
- number of intronse0
- Value
- 0 to 8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the number of introns ranged from 0 to 8”
t0“Gossypium hirsutum”
Claim · model-written summary
Promoter analysis identified 31 cis-acting elements.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Outcome
- cis- acting elementse0
- Value
- 31e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 31 cis- acting elements were identified by analyzing 1,500 bp promoter sequences.”
t0“Gossypium hirsutum”
Claim · model-written summary
Cis-acting elements differed among genes.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Outcome
- cis- acting elementse0
- Direction
- Differencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Differences were found in cis- acting elements among genes.”
t0“Gossypium hirsutum”
Claim · model-written summary
qRT-PCR agreed with earlier transcriptome results, implicating P450 genes in salt tolerance.
- Taxon
- cotton seedlingsGossypium hirsutume0
- Gene
- salt tolerance DEGse0
- Outcome
- salt tolerance of cotton seedlingse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The consistency between qRT-PCR and previous transcriptome analysis of salt tolerance DEGs indicated that they were likely to be involved in the salt tolerance of cotton seedlings.”