Abstract
Aims: To isolate Plant Growth Promoting Bacillus strain from maize roots, to evaluate its biocontrol potentials and to characterize the isolate using16S rRNA sequencing. Place and Duration of Study: Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, between February 2019 and March 2020. Methodology: The isolation of Plant Growth Promoting Rhizobacteria (PGPR) from maize roots was done using Pikovskaya (PVK) agar. Quantitative determination of phosphate was carried out using PVK broth. Evaluations of other plant growth promoting properties were carried out such as IAA, etc. Fusarium and Enterobacter plant pathogens were isolated from diseased maize plants. The in vitro antagonism effects of the PGPR isolates against the pathogens were analyzed using the dual culture plate technique. The pot experiment was carried out in a completely randomized design. Plant characteristics such as plant height, shoot and root weight, chlorophyll content, as well as disease assessment were recorded accordingly. The organisms were identified using phenotypic and molecular methods. Results: Seven PGPR bacteria were isolated from maize (Zea mays) roots using PVK agar. Aneurinibacillus migulanus gave the highest solubilization index of 4.21 while isolate IS48 gave the lowest solubilization index of 1.47. A. migulanus produced IAA, ammonia and cellulase enzyme but no hydrogen cyanide. The organism showed antagonism activity against the two tested phytopathogens. In the pot experiment, A. migulanus treated plants showed a statistically insignificant difference in maize plant height at P=0.05 but gave significant increases in shoot and root wet weights. The organism offered 83.33% and 71.43% protection against Enterobacter and Fusarium pathogens respectively in the pot experiment. Conclusion: A. migulanus solubilized phosphate in addition to other plant growth promoting properties. It showed biocontrol potentials both in vitro and in vivo and thus can be used as substitute for synthetic agrochemicals.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Seven PGPR bacteria were isolated from maize roots.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seven PGPR bacteria were isolated from maize (Zea mays) roots using PVK agar.”
Claim · model-written summary
Aneurinibacillus migulanus had the highest phosphate solubilization index of 4.21.
- Interactor
- Aneurinibacillus migulanusAneurinibacillus migulanuse0
- Outcome
- Aneurinibacillus migulanus gave the highest solubilization indexe0
- Direction
- higheste0
- Value
- 4.21e0
Not reportedInteractor role
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aneurinibacillus migulanus gave the highest solubilization index of 4.21”
t0“Maize Roots”
Claim · model-written summary
Isolate IS48 had the lowest phosphate solubilization index of 1.47.
- Interactor
- isolate IS48e0
- Outcome
- isolate IS48 gave the lowest solubilization indexe0
- Direction
- loweste0
- Value
- 1.47e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolate IS48 gave the lowest solubilization index of 1.47”
t0“Maize Roots”
Claim · model-written summary
A. migulanus produced IAA, ammonia and cellulase enzyme.
- Interactor
- A. migulanusAneurinibacillus migulanuse0
- Outcome
- IAA, ammonia and cellulase enzymee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A. migulanus produced IAA, ammonia and cellulase enzyme but no hydrogen cyanide.”
t0“Maize Roots”
Claim · model-written summary
A. migulanus produced no hydrogen cyanide.
- Interactor
- A. migulanusAneurinibacillus migulanuse0
- Outcome
- no hydrogen cyanidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A. migulanus produced IAA, ammonia and cellulase enzyme but no hydrogen cyanide.”
t0“Maize Roots”
Claim · model-written summary
A. migulanus antagonized both tested phytopathogens in vitro.
- Outcome
- antagonism activity against the two tested phytopathogense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The organism showed antagonism activity against the two tested phytopathogens.”
t0“Maize Roots”
Claim · model-written summary
A. migulanus treated maize plants showed a statistically insignificant height difference.
- Treatment
- A. migulanus treatede0
- Interactor
- A. migulanusAneurinibacillus migulanuse0
- Outcome
- maize plant heighte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A. migulanus treated plants showed a statistically insignificant difference in maize plant height at P=0.05”
Claim · model-written summary
A. migulanus significantly increased maize shoot wet weight.
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gave significant increases in shoot and root wet weights.”
t0“Maize Roots”
Claim · model-written summary
A. migulanus significantly increased maize root wet weight.
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gave significant increases in shoot and root wet weights.”
t0“Maize Roots”
Claim · model-written summary
A. migulanus provided 83.33% protection against Enterobacter in pots.
- Interactor
- Enterobacter
Enterobactere0 - Outcome
- protection against Enterobactere0
- Value
- 83.33%e0
- Setting
- pot experimente0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The organism offered 83.33% and 71.43% protection against Enterobacter and Fusarium pathogens respectively in the pot experiment.”
t0“Maize Roots”
Claim · model-written summary
A. migulanus provided 71.43% protection against Fusarium in pots.
FusariumNot reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The organism offered 83.33% and 71.43% protection against Enterobacter and Fusarium pathogens respectively in the pot experiment.”
t0“Maize Roots”
Claim · model-written summary
The authors proposed A. migulanus as a substitute for synthetic agrochemicals.
- Outcome
- substitute for synthetic agrochemicalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thus can be used as substitute for synthetic agrochemicals.”
t0“Maize Roots”