Abstract
The comparative study of sunflower constant lines resistance to Phoma rot using different methods of artificial inoculation with the disease pathogen was conducted. The most aggressive isolate of the Phoma rot pathogen Plenodomus lindquistii was used for inoculation. It was isolated from the stem of the affected sunflower plant in the breeding nursery of V.S. Pustovoit All-Russian Research Institute of Oil Crops. In a greenhouse conditions, there were used two methods of artificial inoculation with a fungus at bottom of the leaf petiole of sunflower plants at the stage of the first pair of true leaves. In the first case agar pieces with mycelium, pycnidia, and picnospores were applied; in the second one 0.2 ml of the suspension of fungal spores was introduced by syringe injection. In the two control variants, plants were injected with sterile water, or pieces of sterile agar nutrient medium OA (oatmeal agar) were applied to them. The number of replications was four (10 plants each). The plants were cultivated at a variable temperature of 25-30 oС during the day and 25 оС at night (16-hour photoperiod). The cultivation duration was until the budding stage, and we recorded the affection on the 30th day after the start of inoculation. The intensity of the Phoma rot development was determined using a five-point immunological scale. The first method of inoculation (without injury) revealed resistant (VK 900, L 86) and mid-resistant (L 107, L 132) sunflower lines. The second method identified five susceptible (L 2385, L 103, L 136, L 131, L 128) and one highly susceptible (L 82) sunflower lines. In laboratory, the inoculation of the roots of sunflower seedlings by the quick test revealed two constant lines L 116 and L 136 with more than 50 % of plants with healthy root systems or with a small affection degree (1 point). Lines L 2385, L 103, L 131, and L 120 showed the largest number of plants with a medium degree of root affection (2 and 3 points). The resistance of stem and roots to Phoma rot affection in different sunflower lines was manifested both in combination and separately, which should be taken into consideration when selecting genotypes resistant to Phoma rot.
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Claims 10
Claim · model-written summary
Under the first inoculation method, sunflower lines VK 900 and L 86 were resistant to Phoma rot.
- Taxon
- sunflower
Helianthus annuust0 - Material
- VK 900, L 86e1
- Treatment
- first method of inoculation (without injury)e1
- Interactor
- Plenodomus lindquistiiPlenodomus lindquistiie2pathogen
- Outcome
- resistante1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUNFLOWER BREEDING MATERIAL”
e1“The first method of inoculation (without injury) revealed resistant (VK 900, L 86) and mid-resistant (L 107, L 132) sunflower lines”
e2“The most aggressive isolate of the Phoma rot pathogen Plenodomus lindquistii was used for inoculation”
Claim · model-written summary
Under the first inoculation method, sunflower lines L 107 and L 132 were mid-resistant to Phoma rot.
- Taxon
- sunflowerHelianthus annuust0
- Material
- L 107, L 132e1
- Treatment
- first method of inoculation (without injury)e1
- Interactor
- Plenodomus lindquistiiPlenodomus lindquistiie2pathogen
- Outcome
- mid-resistante1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUNFLOWER BREEDING MATERIAL”
e1“The first method of inoculation (without injury) revealed resistant (VK 900, L 86) and mid-resistant (L 107, L 132) sunflower lines”
e2“The most aggressive isolate of the Phoma rot pathogen Plenodomus lindquistii was used for inoculation”
Claim · model-written summary
Under the second inoculation method, sunflower lines L 2385, L 103 and L 136 were susceptible to Phoma rot.
- Taxon
- sunflowerHelianthus annuust0
- Material
- L 2385, L 103, L 136e3
- Treatment
- 0.2 ml of the suspension of fungal spores was introduced by syringe injectione4
- Interactor
- Plenodomus lindquistiiPlenodomus lindquistiie2pathogen
- Outcome
- susceptiblee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUNFLOWER BREEDING MATERIAL”
e3“The second method identified five susceptible (L 2385, L 103, L 136, L 131, L 128) and one highly susceptible (L 82) sunflower lines”
e4“in the second one 0.2 ml of the suspension of fungal spores was introduced by syringe injection”
e2“The most aggressive isolate of the Phoma rot pathogen Plenodomus lindquistii was used for inoculation”
Claim · model-written summary
Under the second inoculation method, sunflower lines L 131 and L 128 were susceptible to Phoma rot.
- Taxon
- sunflowerHelianthus annuust0
- Material
- L 131, L 128e3
- Treatment
- 0.2 ml of the suspension of fungal spores was introduced by syringe injectione4
- Interactor
- Plenodomus lindquistiiPlenodomus lindquistiie2pathogen
- Outcome
- susceptiblee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUNFLOWER BREEDING MATERIAL”
e3“The second method identified five susceptible (L 2385, L 103, L 136, L 131, L 128) and one highly susceptible (L 82) sunflower lines”
e4“in the second one 0.2 ml of the suspension of fungal spores was introduced by syringe injection”
e2“The most aggressive isolate of the Phoma rot pathogen Plenodomus lindquistii was used for inoculation”
Claim · model-written summary
Under the second inoculation method, sunflower line L 82 was highly susceptible to Phoma rot.
- Taxon
- sunflowerHelianthus annuust0
- Material
- L 82e3
- Treatment
- 0.2 ml of the suspension of fungal spores was introduced by syringe injectione4
- Interactor
- Plenodomus lindquistiiPlenodomus lindquistiie2pathogen
- Outcome
- highly susceptiblee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUNFLOWER BREEDING MATERIAL”
e3“The second method identified five susceptible (L 2385, L 103, L 136, L 131, L 128) and one highly susceptible (L 82) sunflower lines”
e4“in the second one 0.2 ml of the suspension of fungal spores was introduced by syringe injection”
e2“The most aggressive isolate of the Phoma rot pathogen Plenodomus lindquistii was used for inoculation”
Claim · model-written summary
In the laboratory quick root test, sunflower lines L 116 and L 136 had more than 50 % of plants with healthy root systems or small root affection.
- Taxon
- sunflowerHelianthus annuust0
- Material
- L 116 and L 136e7
- Organ / stage
- rootse7
- Treatment
- quick teste7
- Outcome
- plants with healthy root systemse7
- Value
- more than 50 %e7
- Setting
- laboratorye7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUNFLOWER BREEDING MATERIAL”
e7“In laboratory, the inoculation of the roots of sunflower seedlings by the quick test revealed two constant lines L 116 and L 136 with more than 50 % of plants with healthy root systems or with a small affection degree (1 point)”
Claim · model-written summary
In the laboratory quick root test, sunflower lines L 2385 and L 103 had the largest number of plants with a medium degree of root affection (2 and 3 points).
- Taxon
- sunflowerHelianthus annuust0
- Material
- L 2385, L 103e3
- Outcome
- largest number of plants with a medium degree of root affectione3
- Value
- 2 and 3 pointse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUNFLOWER BREEDING MATERIAL”
e3“Lines L 2385, L 103, L 131, and L 120 showed the largest number of plants with a medium degree of root affection (2 and 3 points)”
Claim · model-written summary
In the laboratory quick root test, sunflower lines L 131 and L 120 had the largest number of plants with a medium degree of root affection (2 and 3 points).
- Taxon
- sunflowerHelianthus annuust0
- Material
- L 131, and L 120e3
- Outcome
- largest number of plants with a medium degree of root affectione3
- Value
- 2 and 3 pointse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUNFLOWER BREEDING MATERIAL”
e3“Lines L 2385, L 103, L 131, and L 120 showed the largest number of plants with a medium degree of root affection (2 and 3 points)”
Claim · model-written summary
Resistance of stem and roots to Phoma rot in different sunflower lines was manifested both in combination and separately.
- Taxon
- sunflowerHelianthus annuust0
- Organ / stage
- stem and rootse5
- Outcome
- resistance of stem and rootse5
- Qualifier
- both in combination and separatelye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUNFLOWER BREEDING MATERIAL”
e5“The resistance of stem and roots to Phoma rot affection in different sunflower lines was manifested both in combination and separately”
Claim · model-written summary
Stem and root resistance to Phoma rot should be taken into consideration when selecting sunflower genotypes.
- Taxon
- sunflowerHelianthus annuust0
- Outcome
- selecting genotypes resistant to Phoma rote6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUNFLOWER BREEDING MATERIAL”
e6“which should be taken into consideration when selecting genotypes resistant to Phoma rot”