Abstract
Pod shattering can lead to devastating yield loss of soybean and has been a negatively selected trait in soybean domestication and breeding. Nevertheless, a significant portion of soybean cultivars are still pod shattering-susceptible, limiting their regional and climatic adaptabilities. Here we performed genetic diagnosis on the shattering-susceptible trait of a national registered cultivar, Huachun6 (HC6), and found that HC6 carries the susceptible genotype of a candidate Pod dehiscence 1 ( PDH1 ) gene, which exists in a significant portion of soybean cultivars. We next performed genome editing on PDH1 gene by clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9). In T 2 progenies, several transgene-free lines with pdh1 mutations were characterized without affecting major agronomic traits. The pdh1 mutation significantly improved the pod shattering resistance which is associated with aberrant lignin distribution in inner sclerenchyma. Our work demonstrated that precision breeding by genome editing on PDH1 holds great potential for precisely improving pod shattering resistance and adaptability of soybean cultivars.
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Claims 5
Claim · model-written summary
Huachun6 carries a susceptible PDH1 genotype also present in many soybean cultivars.
- Taxon
- soybean
Glycine maxe0 - Material
- HC6e0
- Gene
- PDH1e0
- Outcome
- susceptible genotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HC6 carries the susceptible genotype of a candidate Pod dehiscence 1 ( PDH1 ) gene, which exists in a significant portion of soybean cultivars.”
e1“national registered cultivar, Huachun6 (HC6)”
Claim · model-written summary
Several transgene-free pdh1 mutant lines were obtained in T2 progenies.
- Taxon
- soybeanGlycine maxe1
- Gene
- pdh1e0
- Outcome
- transgene-free lines with pdh1 mutationse0
- Value
- severale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In T 2 progenies, several transgene-free lines with pdh1 mutations were characterized”
e1“significant portion of soybean cultivars.”
Claim · model-written summary
The pdh1 mutant lines did not alter major agronomic traits.
- Taxon
- soybeanGlycine maxe1
- Treatment
- pdh1 mutationse0
- Outcome
- major agronomic traitse0
- Direction
- without affectinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several transgene-free lines with pdh1 mutations were characterized without affecting major agronomic traits.”
e1“significant portion of soybean cultivars.”
Claim · model-written summary
The pdh1 mutation significantly improved pod-shattering resistance.
- Taxon
- soybeanGlycine maxe1
- Treatment
- pdh1 mutatione0
- Gene
- pdh1e0
- Outcome
- pod shattering resistancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pdh1 mutation significantly improved the pod shattering resistance”
e1“significant portion of soybean cultivars.”
Claim · model-written summary
Improved shattering resistance was associated with aberrant lignin distribution in inner sclerenchyma.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- inner sclerenchymae0
- Gene
- pdh1e0
- Compound
- lignine0
- Outcome
- pod shattering resistancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pdh1 mutation significantly improved the pod shattering resistance which is associated with aberrant lignin distribution in inner sclerenchyma.”
e1“significant portion of soybean cultivars.”
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