Abstract
Different lesions were frequently observed on leaves and petioles of wheat plants (Triticum aestivum L.) during 2020 growing season at Beni Suef governorate, Egypt. The lesions were brown to dark brown with a central white spot and a chlorotic halo (Fig. 1 A & B). The lesions were round to oval in shape and ranged in diameter from 5 to 14 mm on the leaves. Lesion scratched were cultured on potato dextrose agar (PDA) and incubated at 25°C for 7 days. The isolated fungus was identified according to its morphological and microscopic characteristics. Conidia of isolated fungi were muri form, mainly oblong to ovoid, but rarely almost globose, subhyaline to various colors of brown, mostly constricted at the median septum, and measured 1.74 - 2.86 × 12 - 14.8 µm (average 2.3 × 13.4 µm) (Fig. 1 C & D). Moreover, total DNA was isolated from mycelium removed from 7-day-old colonies of single-spore isolates grown on PDA using DNAzol reagent (Thermo Fisher Scientific, U.S.A.), and PCR amplification of the internal transcribed spacer (ITS) rDNA region was performed with primers ITS1/ITS4 (White et al., 1990), The obtained sequences were deposited in GenBank with accession number MZ944879. According to BLAST search in GenBank, the MZ944879 ITS sequence had 100% similarity to sequences MH628100, MH628101, MH628102, and MH628104, respectively, showed 100% identity of Stemphylium vesicarium. Pathogenicity test was confirmed by spraying a spore suspension (105 conidia ml–1) produced on 7-day-old culture on healthy wheat plants (cv. Beni Suef 5), at the 5-true-leaf stage. An equal number of plants were sprayed with sterile distilled water as controls, under greenhouse conditions at Smart Agriculture Clinic Project, Beni Suef governorate, Egypt. Disease symptoms appeared on wheat, which resembled those observed under natural infection conditions. S. vesicarium was consistently re-isolated from artificially inoculated wheat tissues, thus verifying Koch’s postulates. As the authors are far aware, this is the first report of Stemphylium leaf spot disease of wheat caused by S. vesicarium in Egypt.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Wheat leaves and petioles in Egypt bore brown lesions with white centers and chlorotic halos.
- Taxon
- wheat
Triticum aestivume0 - Organ / stage
- leaves and petiolese0
- Outcome
- lesions were brown to dark brown with a central white spot and a chlorotic haloe0
- Setting
- Beni Suef governorate, Egypte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Different lesions were frequently observed on leaves and petioles of wheat plants (Triticum aestivum L.) during 2020 growing season at Beni Suef governorate, Egypt. The lesions were brown to dark brown with a central white spot and a chlorotic halo”
Claim · model-written summary
Wheat leaf lesions were round to oval and 5 to 14 mm in diameter.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- leavese0
- Outcome
- lesions were round to oval in shapee0
- Value
- 5 to 14 mme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lesions were round to oval in shape and ranged in diameter from 5 to 14 mm on the leaves”
t0“Wheat”
Claim · model-written summary
The isolate from wheat lesions was identified as Stemphylium vesicarium by ITS sequence identity.
- Taxon
- WheatTriticum aestivumt0
- Interactor
- Stemphylium vesicarium
.jpg?width=60)
Stemphylium vesicariume0pathogen - Outcome
- 100% identity of Stemphylium vesicariume0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“the MZ944879 ITS sequence had 100% similarity to sequences MH628100, MH628101, MH628102, and MH628104, respectively, showed 100% identity of Stemphylium vesicarium”
t0“Wheat”
Claim · model-written summary
Inoculated wheat developed disease symptoms resembling natural infection.
- Taxon
- wheatTriticum aestivume0
- Material
- Beni Suef 5e0
- Organ / stage
- 5-true-leaf stagee0
- Treatment
- spraying a spore suspension (105 conidia ml–1)e0
- Outcome
- Disease symptoms appeared on wheate0
- Comparator
- sterile distilled watere0
- Setting
- greenhouse conditionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pathogenicity test was confirmed by spraying a spore suspension (105 conidia ml–1) produced on 7-day-old culture on healthy wheat plants (cv. Beni Suef 5), at the 5-true-leaf stage. An equal number of plants were sprayed with sterile distilled water as controls, under greenhouse conditions at Smart Agriculture Clinic Project, Beni Suef governorate, Egypt. Disease symptoms appeared on wheat, which resembled those observed under natural infection conditions”
Claim · model-written summary
Stemphylium vesicarium was re-isolated from inoculated wheat tissue, confirming pathogenicity.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- wheat tissuese0
- Interactor
- S. vesicariumStemphylium vesicariume0pathogen
- Outcome
- S. vesicarium was consistently re-isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S. vesicarium was consistently re-isolated from artificially inoculated wheat tissues, thus verifying Koch’s postulates”
Claim · model-written summary
The authors report Stemphylium leaf spot of wheat caused by S. vesicarium in Egypt for the first time.
- Taxon
- wheatTriticum aestivume0
- Interactor
- S. vesicariumStemphylium vesicariume0pathogen
- Outcome
- first report of Stemphylium leaf spot diseasee0
- Setting
- Egypte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this is the first report of Stemphylium leaf spot disease of wheat caused by S. vesicarium in Egypt”
The same questions in other papers
application · disease · Triticum aestivum (wheat)Positive and negative results
16 papers in the same direction as this paper · 83 in the opposite direction
- negative
- positive
- positive
96 papers more in this group
See all 100 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.