Paper · 2017 · American Research Journal of Biosciences

Exopolysaccharides production by Mesorhizobium loti: effect of carbon source and pH

Rutilene Roll; Jssica Dutra Vieira; Joo Sales; et al.

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Open access · other-oa

Abstract

Interest in culturing microorganisms for production of valuable polysaccharides has greatly increased and attracted worldwide attention due to their novel and exclusive physical properties. We report the production of exopolysaccharides (EPSs) by two strains of Mesorhizobium loti (SEMIA806 and SEMIA816) under two different pH conditions and using three different sources of carbon. From 0.105 to 0.599 g.L -1 of EPS was produced by bacteria growing in medium using dextrose as carbon source and from 0.332 to 2.04 g.L -1 of EPS when the sucrose was the carbon source employed. The use of mannitol as carbon source resulted in the lower rate of EPS production, not exceeding 0.001 g.L -1 . In both, pH 5.5 and pH 7.0, the amount of EPS produced was higher for SEMIA816 than for SEMIA806. Both strains produced higher amount of EPSs at pH 5.5 and using sucrose as carbon source. Since none of the species of this genus have yet been shown to be pathogenic M. loti SEMIA806 and SEMIA816 can be included among the potential sources of promising biopolymers using stressful growing conditions.

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