Paper · 2020 · Letters in Applied NanoBioScience

Estimation of Genetic Diversity in Piper betle L. Based on the Analysis of Morphological and Molecular Markers

Biswajit Patra; Alok Sahu; Ramovatar Meena; et al.

6
claims
12
verbatim quotes
2
citations
1
research areas
✓
Open access

Abstract

Paan (Piper betle L., family Piperaceae) is an important evergreen cultivated crop of India. We estimated genetic diversity by using morphological and RAPD markers in four cultivars, namely Piper betle L. var. Bali, var. Chandrakanta, var. Jhanji and var. Kala Bangla. We had used morphological markers like leaf length, leaf width, and petiole size. By using the morphological markers, hierarchical cluster analysis was carried out, which grouped these four cultivars into two major clusters. In molecular marker analysis, a total of ten RAPD primers used, generating 43 number of amplified bands. Among them, 15 number of polymorphic bands and seven unique bands were found. The genetic diversity and relatedness among the four cultivars were computed using Jaccard’s similarity coefficient. The dendrogram grouped all the four cultivars into two main clusters. This RAPD banding patterns can be useful for genetic diversity studies, for cultivar selection, and to marker assist breeding programs.

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Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Morphological markers grouped the four Piper betle cultivars into two major clusters.

Taxon
Piper betlePiper betlet0
Outcome
two major clusterse1
Qualifier
By using the morphological markerse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Piper betle
Piper betle · TaxonUndeka 11 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Estimation of Genetic Diversity in Piper betle L.”

✓ located verbatim in the source · title, characters 0–49 · show in text ↑
e1

“By using the morphological markers, hierarchical cluster analysis was carried out, which grouped these four cultivars into two major clusters”

✓ located verbatim in the source · abstract, characters 351–492 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Ten RAPD primers generated 43 amplified bands.

Taxon
Piper betlePiper betlet0
Outcome
amplified bandse2
Value
43e2
Qualifier
ten RAPD primerse2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Piper betle
Piper betle · TaxonUndeka 11 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Estimation of Genetic Diversity in Piper betle L.”

✓ located verbatim in the source · title, characters 0–49 · show in text ↑
e2

“a total of ten RAPD primers used, generating 43 number of amplified bands”

✓ located verbatim in the source · abstract, characters 524–597 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Of the RAPD bands, 15 were polymorphic.

Taxon
Piper betlePiper betlet0
Outcome
polymorphic bandse3
Value
15e3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Piper betle
Piper betle · TaxonUndeka 11 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Estimation of Genetic Diversity in Piper betle L.”

✓ located verbatim in the source · title, characters 0–49 · show in text ↑
e3

“Among them, 15 number of polymorphic bands and seven unique bands were found”

✓ located verbatim in the source · abstract, characters 599–675 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Seven unique RAPD bands were found.

Taxon
Piper betlePiper betlet0
Outcome
unique bandse3
Value
sevene3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Piper betle
Piper betle · TaxonUndeka 11 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Estimation of Genetic Diversity in Piper betle L.”

✓ located verbatim in the source · title, characters 0–49 · show in text ↑
e3

“Among them, 15 number of polymorphic bands and seven unique bands were found”

✓ located verbatim in the source · abstract, characters 599–675 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The dendrogram grouped the four cultivars into two main clusters.

Taxon
Piper betlePiper betlet0
Outcome
two main clusterse4
Qualifier
dendrograme4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Piper betle
Piper betle · TaxonUndeka 11 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Estimation of Genetic Diversity in Piper betle L.”

✓ located verbatim in the source · title, characters 0–49 · show in text ↑
e4

“The dendrogram grouped all the four cultivars into two main clusters”

✓ located verbatim in the source · abstract, characters 794–862 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

RAPD banding patterns can be useful for genetic diversity studies, cultivar selection and marker-assisted breeding in Piper betle.

Taxon
Piper betlePiper betlet0
Outcome
genetic diversity studiese5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Piper betle
Piper betle · TaxonUndeka 11 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Estimation of Genetic Diversity in Piper betle L.”

✓ located verbatim in the source · title, characters 0–49 · show in text ↑
e5

“This RAPD banding patterns can be useful for genetic diversity studies, for cultivar selection, and to marker assist breeding programs”

✓ located verbatim in the source · abstract, characters 864–998 · show in text ↑