Abstract
TREPTOMYCES vinaceusdrappus SA was subjected to …...submerged fermentation under optimum conditions, at 30C and pH 7.0 in presence of 1% casein & 0.5% maltose and 0.5 % yeast & 1% sucrose for 6 and 5 days for protease and lipase production, respectively, under static conditions. Partial purification of protease and lipase were carried out by70% ammonium sulphate precipitation, dialysis, Sephadex G100 and finally Sephadex G50. The specific activities of protease and lipase at final purification step were 116.7 and 104.9 U mg-1 protein compared to 8.4 and 8.9 U mg-1 crude proteins, with yield 37 and 29 %, respectively. Both enzymes gave single homogenous band of 71 kDa by SDS- PAGE. Also, their optimum pH was 7.0 and they had pH stability from 7.0 – 11.0. The maximum enzyme activities obtained at 55oC and 60°C with Tm (half life temperature) values 86.5 and 83.9ەC for protease and lipase, respectively. The penetration of newly hatched larvae of pink bollworm into the cotton bolls after being dipped in crude or purified protease and lipase each alone or in mixture (v/v) were studied. The results revealed that the penetration percent decreased in pure enzymes than crude. Treatment with the mixed crude enzymes decreased the percentage of penetration than of each enzyme alone. Also, crude lipase , protease and their mixture had repellent action for pink bollworm moths. Moreover, there was highly significant effect on the deformed adult percentage compared with control since the pure enzyme had effective role than crude enzyme.
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Claims 6
Claim · model-written summary
Purified enzymes reduced pink bollworm penetration more than crude enzymes.
- Taxon
- cotton
Gossypium hirsutume1 - Treatment
- pure enzymese0
- Outcome
- penetration percente0
- Direction
- decreasede0
- Comparator
- crudee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the penetration percent decreased in pure enzymes than crude”
e1“cotton bolls”
Claim · model-written summary
Mixed crude enzymes reduced penetration more than either enzyme alone.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- mixed crude enzymese0
- Outcome
- percentage of penetratione0
- Direction
- decreasede0
- Comparator
- each enzyme alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with the mixed crude enzymes decreased the percentage of penetration than of each enzyme alone”
e1“cotton bolls”
Claim · model-written summary
Crude lipase repelled pink bollworm moths.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- crude lipasee0
- Outcome
- repellent action for pink bollworm mothse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“crude lipase , protease and their mixture had repellent action for pink bollworm moths”
e1“cotton bolls”
Claim · model-written summary
Crude protease repelled pink bollworm moths.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- proteasee0
- Outcome
- repellent action for pink bollworm mothse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protease and their mixture had repellent action for pink bollworm moths”
e1“cotton bolls”
Claim · model-written summary
The crude enzyme mixture repelled pink bollworm moths.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- their mixturee0
- Outcome
- repellent action for pink bollworm mothse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their mixture had repellent action for pink bollworm moths”
e1“cotton bolls”
Claim · model-written summary
Purified enzyme treatment had a highly significant effect on deformed adults versus control.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- pure enzymee0
- Outcome
- deformed adult percentagee0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was highly significant effect on the deformed adult percentage compared with control since the pure enzyme had effective role than crude enzyme”
e1“cotton bolls”