Paper · 2022 · The Journal of Experimental Life Sciences

Synergism of Lecanicillium lecanii (Zimm) and Chromolaena odorata L. Leaf Extract to Control Aphis gossypii (Glover) in Chili Plants

Wiwik Nurhayati; Nanang Tri Haryadi

5
claims
11
verbatim quotes
2
citations
2
research areas
✓
Open access · cc-by

Abstract

The purpose of the study was to determine the effect of the appropriate and effective application of the L. lecanii fungus and C. odorata L. leaf extract in increasing the mortality of A. gossypii. The study was divided into two stages. The first stage was in vitro test of L. lecanii synergism plus C. odorata L. leaf extract in PDA media with four treatments, namely LK0= L. lecanii 107 conidia.mL-1 plus 0% C. odorata L. leaf extract, LK1= L. lecanii 107 conidia.mL-1 plus 10% C. odorata L. leaf extract, LK2= L. lecanii 107 conidia.mL-1 plus 25% C. odorata L. leaf extract; LK3= L. lecanii 107 conidia.mL-1 plus 40% C. odorata L. leaf extract. The second stage was the toxicity test of the application of L. lecanii suspension and C. odorata L. leaf extract on mortality of A. gossypii. The toxicity test was based on the results of the synergism test, where the addition of C. odorata L. leaf extract to L. lecanii growing media showed incompatible results. Therefore, the toxicity test was carried out separately with five treatments, namely H0= Control (aqua dest); H1= Conidia suspension L. lecanii 107 conidia mL-1; H2= 10% C. odorata leaf extract; H3= 25% leaf extract of C. odorata; H4= 40% C. odorata leaf extract. The results showed that the compatibility test of L. lecanii with leaf extract of C. odorata L. was incompatible and classified as toxic. The addition of C. odorata L. leaf extract in concentrations of 10%, 25%, and 40% could significantly inhibit colony growth, sporulation, and conidia viability of L. lecanii, with a higher level of inhibition as the concentration of C. odorata L. leaf extract, was added. The toxicity test of a separate application of C. odorata L. leaf extract and L. lecanii suspension had a significant effect on mortality of 3rd instar nymph A. gossypii, with the highest mean mortality found in a single application of 40% C. odorata L. leaf extract with an average mortality of 100% at 96 HAA(Hours After Application) observations. Keywords: A. gossypii, C. odorata L. leaf extract, L. lecanii, synergism.

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Claims 5

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Chromolaena leaf extract was incompatible with Lecanicillium and toxic in the compatibility test.

Taxon
Chili Plantst0
Interactor
L. lecaniiAkanthomyces lecaniie0biocontrol_agent
Outcome
compatibility test of L. lecanii with leaf extract of C. odorata L.e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Akanthomyces lecanii
Akanthomyces lecanii · InteractorStephen Thorpe · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The results showed that the compatibility test of L. lecanii with leaf extract of C. odorata L. was incompatible and classified as toxic.”

✓ located verbatim in the source · abstract, characters 1227–1364 · show in text ↑
t0

“Chili Plants”

✓ located verbatim in the source · title, characters 120–132 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Increasing extract concentration significantly inhibited Lecanicillium colony growth.

Taxon
Chili Plantst0
Treatment
C. odorata L. leaf extract in concentrations of 10%, 25%, and 40%e0
Interactor
L. lecaniiAkanthomyces lecaniie0biocontrol_agent
Outcome
colony growthe0
Direction
inhibite0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Akanthomyces lecanii
Akanthomyces lecanii · InteractorStephen Thorpe · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The addition of C. odorata L. leaf extract in concentrations of 10%, 25%, and 40% could significantly inhibit colony growth, sporulation, and conidia viability of L. lecanii, with a higher level of inhibition as the concentration of C. odorata L. leaf extract, was added.”

✓ located verbatim in the source · abstract, characters 1365–1636 · show in text ↑
t0

“Chili Plants”

✓ located verbatim in the source · title, characters 120–132 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Increasing extract concentration significantly inhibited Lecanicillium sporulation and conidia viability.

Taxon
Chili Plantst0
Treatment
C. odorata L. leaf extract in concentrations of 10%, 25%, and 40%e0
Interactor
L. lecaniiAkanthomyces lecaniie0biocontrol_agent
Outcome
sporulation, and conidia viability of L. lecaniie0
Direction
inhibite0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Akanthomyces lecanii
Akanthomyces lecanii · InteractorStephen Thorpe · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The addition of C. odorata L. leaf extract in concentrations of 10%, 25%, and 40% could significantly inhibit colony growth, sporulation, and conidia viability of L. lecanii, with a higher level of inhibition as the concentration of C. odorata L. leaf extract, was added.”

✓ located verbatim in the source · abstract, characters 1365–1636 · show in text ↑
t0

“Chili Plants”

✓ located verbatim in the source · title, characters 120–132 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Separate extract and fungal applications significantly affected third instar aphid mortality.

Taxon
Chili Plantst0
Treatment
separate application of C. odorata L. leaf extract and L. lecanii suspensione0
Interactor
A. gossypiiAphis gossypiie0pest
Second interactor
L. lecaniiAkanthomyces lecaniie0biocontrol_agent
Outcome
mortality of 3rd instar nymph A. gossypiie0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Aphis gossypii
Aphis gossypii · InteractorDr Lewis Wilson, CSIRO · CC BY 3.0 · Wikimedia Commons ↗
Akanthomyces lecanii
Akanthomyces lecanii · Second interactorStephen Thorpe · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The toxicity test of a separate application of C. odorata L. leaf extract and L. lecanii suspension had a significant effect on mortality of 3rd instar nymph A. gossypii”

✓ located verbatim in the source · abstract, characters 1637–1806 · show in text ↑
t0

“Chili Plants”

✓ located verbatim in the source · title, characters 120–132 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The 40% extract alone produced the highest mean aphid mortality, 100% at 96 hours.

Taxon
Chili Plantst0
Treatment
single application of 40% C. odorata L. leaf extracte0
Interactor
Aphis gossypiiAphis gossypiit1pest
Outcome
mean mortalitye0
Direction
higheste0
Value
100%e0
Qualifier
96 HAA(Hours After Application)e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Aphis gossypii
Aphis gossypii · InteractorDr Lewis Wilson, CSIRO · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the highest mean mortality found in a single application of 40% C. odorata L. leaf extract with an average mortality of 100% at 96 HAA(Hours After Application) observations.”

✓ located verbatim in the source · abstract, characters 1813–1986 · show in text ↑
t0

“Chili Plants”

✓ located verbatim in the source · title, characters 120–132 · show in text ↑
t1

“Aphis gossypii (Glover)”

✓ located verbatim in the source · title, characters 93–116 · show in text ↑

botanical · Aphis gossypii · Capsicum (peppers)Results agree

2 papers: 2 positive, 0 negative or no effect

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  1. positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.