Abstract
There are a number of questions that have remained relevant for a long time when using inoculants based on nodule bacteria. It has been shown that exopolysaccharides (EPS) producing bacterial cells play one of the key roles during interaction and during signal transmission in legume-rhizobial symbiosis, namely, bacterial survival, virulence of rhizobia, shelf life on inoculated seeds and in soil, and efficiency created legume-rhizobial system. In particular, it was found that new strains of soybean nodule bacteria Bradyrhizobium japonicum LG 2 and LG 5 synthesize EPS at 3.2 and 2.8 g/l, with an optical density above 0.8 rel. units, the viscosity of the culture liquidwithin 270-302 mPa s. The high level of synthesis and the chemical composition of EPS of the studied strains ensured the preservation of the viability of bacterial cells on the surface of seeds and on a solid medium. Inoculation of soybean seeds with a culture liquid containing EPS of strains LG 2 and LG 5 ensured the formation of an effective legume-rhizobial system, which is confirmed by the formation of 18-21 nodules / plant of soybeans with a nitrogenase activity of at least 2.9 mol C2H4 / plant per hour. A graph model has been developed, which proves the direct dependence of the effect of EPS on the number of nodules formed on the roots, the level of nitrogenase activity of the legume-rhizobial system and their indirect effect on the yield and protein content in soybean grain. Prospects isolated by strains LG 2 and LG 5 of B. japonicum for biotechnological production of liquid and gel biopreparations.
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Claims 6
Claim · model-written summary
Bradyrhizobium japonicum LG 2 produced 3.2 g/l exopolysaccharides.
- Taxon
- Bradyrhizobium japonicum
Bradyrhizobium japonicume0 - Material
- LG 2e0
- Outcome
- synthesize EPSe0
- Value
- 3.2e0
- Qualifier
- 2.8 g/le0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“new strains of soybean nodule bacteria Bradyrhizobium japonicum LG 2 and LG 5 synthesize EPS at 3.2 and 2.8 g/l, with an optical density above 0.8 rel. units, the viscosity of the culture liquidwithin 270-302 mPa s.”
Claim · model-written summary
Bradyrhizobium japonicum LG 5 produced 2.8 g/l exopolysaccharides.
- Taxon
- Bradyrhizobium japonicumBradyrhizobium japonicume0
- Material
- LG 5e0
- Outcome
- synthesize EPSe0
- Value
- 2.8 g/le0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“new strains of soybean nodule bacteria Bradyrhizobium japonicum LG 2 and LG 5 synthesize EPS at 3.2 and 2.8 g/l, with an optical density above 0.8 rel. units, the viscosity of the culture liquidwithin 270-302 mPa s.”
Claim · model-written summary
Exopolysaccharide synthesis and composition preserved bacterial cell viability on seeds and solid medium.
Verbatim
e0“The high level of synthesis and the chemical composition of EPS of the studied strains ensured the preservation of the viability of bacterial cells on the surface of seeds and on a solid medium.”
Claim · model-written summary
Inoculation with LG 2 and LG 5 culture liquid yielded 18-21 soybean nodules per plant.
- Taxon
- soybean
Glycine maxe0 - Treatment
- Inoculation of soybean seeds with a culture liquid containing EPS of strains LG 2 and LG 5e0
- Interactor
- strains LG 2 and LG 5e0symbiont
- Outcome
- nodules / plant of soybeanse0
- Value
- 18-21 nodules / plante0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation of soybean seeds with a culture liquid containing EPS of strains LG 2 and LG 5 ensured the formation of an effective legume-rhizobial system, which is confirmed by the formation of 18-21 nodules / plant of soybeans with a nitrogenase activity of at least 2.9 mol C2H4 / plant per hour.”
Claim · model-written summary
Inoculation with LG 2 and LG 5 culture liquid yielded nitrogenase activity of at least 2.9 mol C2H4 per plant per hour.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Inoculation of soybean seeds with a culture liquid containing EPS of strains LG 2 and LG 5e0
- Interactor
- strains LG 2 and LG 5e0symbiont
- Outcome
- nitrogenase activitye0
- Value
- 2.9 mol C2H4 / plant per houre0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation of soybean seeds with a culture liquid containing EPS of strains LG 2 and LG 5 ensured the formation of an effective legume-rhizobial system, which is confirmed by the formation of 18-21 nodules / plant of soybeans with a nitrogenase activity of at least 2.9 mol C2H4 / plant per hour.”
Claim · model-written summary
The graph model linked exopolysaccharides directly to nodule number and nitrogenase activity and indirectly to yield and grain protein.
- Taxon
- soybeanGlycine maxe0
- Outcome
- direct dependence of the effect of EPS on the number of nodules formed on the roots, the level of nitrogenase activity of the legume-rhizobial systeme0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A graph model has been developed, which proves the direct dependence of the effect of EPS on the number of nodules formed on the roots, the level of nitrogenase activity of the legume-rhizobial system and their indirect effect on the yield and protein content in soybean grain.”
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See all 310 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.