Abstract
A detailed study of the Turkish endemic Gypsophila pilulifera were presented by both morphological and molecular investigations of several sites (each one divided in four biotopes). The characteristics were investigated using statistic analyses, i.e. t-Test and One-way ANOVA tests, as well as Discriminant analysis (á=0.05). Some differences were detected among the individuals in the locations stufied where the species was distributed in terms of morphological characteristics and obvious similarities/differences were determined among the biotopes as a result of the discriminant analysis. Morphological characterization was supported by molecular data. Four individuals from two different locations were analyzed by PCR using twenty-four ISSR primers. Analysis of PCR products on agarose gel yielded three primary reproductive band profiles, and the similarity frequencies calculated with the Jaccard coefficient were found to be among 0.229 and 0.769. In this context, according to the results, there was no difference in species, subspecies or variety level between individuals between the two locations. But the obtained molecular differences support the morphological differences. It is predicted that this may change towards speciation as ecological changes increase in the locations where the species is distributed.
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Claims 6
Claim · model-written summary
Morphological characteristics differed among individuals at study locations.
- Taxon
- Gypsophila piluliferaGypsophila piluliferat0
- Outcome
- morphological characteristicse0
Verbatim
e0“Some differences were detected among the individuals in the locations stufied where the species was distributed in terms of morphological characteristics”
t0“Gypsophila pilulifera”
Claim · model-written summary
Biotopes showed morphological similarities and differences in discriminant analysis.
- Taxon
- Gypsophila piluliferaGypsophila piluliferat0
- Outcome
- similarities/differencese0
Verbatim
e0“obvious similarities/differences were determined among the biotopes as a result of the discriminant analysis”
t0“Gypsophila pilulifera”
Claim · model-written summary
PCR analysis yielded three primary reproductive band profiles.
- Taxon
- Gypsophila piluliferaGypsophila piluliferat0
- Outcome
- reproductive band profilese0
- Value
- threee0
Verbatim
e0“Analysis of PCR products on agarose gel yielded three primary reproductive band profiles”
t0“Gypsophila pilulifera”
Claim · model-written summary
Jaccard similarity frequencies ranged from 0.229 to 0.769.
- Taxon
- Gypsophila piluliferaGypsophila piluliferat0
- Outcome
- similarity frequenciese0
- Value
- 0.229 and 0.769e0
Verbatim
e0“similarity frequencies calculated with the Jaccard coefficient were found to be among 0.229 and 0.769”
t0“Gypsophila pilulifera”
Claim · model-written summary
Individuals at two locations showed no difference at species, subspecies or variety level.
- Taxon
- Gypsophila piluliferaGypsophila piluliferat0
- Outcome
- no difference in species, subspecies or variety levele0
Verbatim
e0“there was no difference in species, subspecies or variety level between individuals between the two locations”
t0“Gypsophila pilulifera”
Claim · model-written summary
Molecular differences supported the morphological differences.
- Taxon
- Gypsophila piluliferaGypsophila piluliferat0
- Material
- molecular differencese0
- Outcome
- morphological differencese0
Not reportedDirection
Verbatim
e0“the obtained molecular differences support the morphological differences”
t0“Gypsophila pilulifera”