Paper · 1985 · Journal of the American Society for Horticultural Science

Production of Synthetic Seeds by Encapsulating Asexual Embryos of Carrot

S. L. Kitto; Jules Janick

2
claims
4
verbatim quotes
100
citations
2
research areas
—
Not open access

Abstract

Abstract Synthetic seed coats were applied to asexual embryos of carrot ( Daucus carota L.) by mixing equal volumes of embryo suspension and a 5% (w/v) solution of polyethylene oxide (Polyox WSR-N 750) and dispensing 0.2 ml drops of this mixture onto teflon sheets. Drops dried to form detachable wafers consisting of embryo suspension embedded in Polyox. Embryo survival after drying was determined by redissolving wafers in embryogenic medium and culturing the rehydrated embryo suspension on filter paper supports in petri dishes for 2-3 weeks. When dried to constant weight (6.5 hr) 3% of asexual embryos coated with 2.5% Polyox survived encapsulation, whereas survival of uncoated embryos was nil. Pretreating the embryogenic suspension with 10 −6 m abscisic acid (ABA) during the 14 day embryo induction phase increased coated embryo survival to 40% of the initial number of embryos.

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Claims 2

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Polyox-coated carrot embryos had 3% survival after drying versus nil for uncoated embryos.

Taxon
CarrotDaucus carotat0
Treatment
2.5% Polyoxe0
Outcome
asexual embryos coated with 2.5% Polyox survived encapsulatione0
Direction
survivede0
Value
3%e0
Qualifier
survival of uncoated embryos was nile0
Comparator
uncoated embryose0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“When dried to constant weight (6.5 hr) 3% of asexual embryos coated with 2.5% Polyox survived encapsulation, whereas survival of uncoated embryos was nil”

✓ located verbatim in the source · abstract, characters 548–701 · show in text ↑
t0

“Carrot”

✓ located verbatim in the source · title, characters 66–72 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

ABA pretreatment increased coated embryo survival to 40%.

Taxon
CarrotDaucus carotat0
Treatment
10 −6 m abscisic acid (ABA)e0
Outcome
coated embryo survivale0
Direction
increasede0
Value
40%e0
Qualifier
during the 14 day embryo induction phasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Pretreating the embryogenic suspension with 10 −6 m abscisic acid (ABA) during the 14 day embryo induction phase increased coated embryo survival to 40% of the initial number of embryos”

✓ located verbatim in the source · abstract, characters 703–888 · show in text ↑
t0

“Carrot”

✓ located verbatim in the source · title, characters 66–72 · show in text ↑

(+)-abscisic acid · survival · Daucus carota (carrot)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.