Abstract
In plants, calcium-dependent protein kinases (CDPKs) play crucial role in the regulation of growth and development in response to various stresses. They act as sensor-transducers in decoding the calcium (Ca2+) ions associated with multiple signal transduction pathways. The plant CDPK isoforms are encoded by the nuclear multigene families. Some CDPK isoforms were reported earlier in potato; but their three-dimensional (3-D) structures were not predicted. Based on the RT-PCR approach using tuber total RNA, a 1560-bp cDNA clone encoding 515-aa CDPK2 isoform (designated StCDPK2) was isolated and characterized from the potato cultivar Kufri Chipsona-1(KC-1). A thorough sequence analysis revealed both sequence identities and crucial motifs in the potato CDPKs. Semi-quantitative RT-PCR approach was adopted to show the expression patterns of StCDPK2 in different potato organs grown under field condition. The three-dimensional (3-D) structures of potato CDPK1−3 were predicted by a composite approach using ab initio modeling, and further validated and assessed by Ramachandran plot analyses. In addition, a number of the CDPK isoforms were retrieved from the potato genome sequence database; their probable biochemical properties and functional aspects were predicted based on both bioinformatics tools and published reports.
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Claims 4
Claim · model-written summary
A 1560-bp cDNA encoding 515-aa StCDPK2 was isolated from potato cultivar Kufri Chipsona-1.
- Taxon
- potato
Solanum tuberosume0 - Material
- Kufri Chipsona-1e0
- Gene
- StCDPK2e0
- Outcome
- was isolated and characterizede0
- Value
- 1560-bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 1560-bp cDNA clone encoding 515-aa CDPK2 isoform (designated StCDPK2) was isolated and characterized from the potato cultivar Kufri Chipsona-1(KC-1).”
Claim · model-written summary
Sequence analysis identified identities and crucial motifs in potato CDPKs.
- Taxon
- potatoSolanum tuberosume0
- Gene
- CDPKse0
- Outcome
- sequence identities and crucial motifse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A thorough sequence analysis revealed both sequence identities and crucial motifs in the potato CDPKs.”
Claim · model-written summary
Three-dimensional structures of potato CDPK1−3 were predicted and assessed with Ramachandran plots.
- Taxon
- potatoSolanum tuberosume0
- Gene
- CDPK1−3e0
- Outcome
- three-dimensional (3-D) structurese0
- Value
- 3-De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The three-dimensional (3-D) structures of potato CDPK1−3 were predicted by a composite approach using ab initio modeling, and further validated and assessed by Ramachandran plot analyses.”
Claim · model-written summary
CDPK isoforms were retrieved from the potato genome and their probable biochemical properties and functions predicted.
- Taxon
- potatoSolanum tuberosume0
- Gene
- CDPK isoformse0
- Outcome
- probable biochemical properties and functional aspectse0
- Value
- a number ofe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a number of the CDPK isoforms were retrieved from the potato genome sequence database; their probable biochemical properties and functional aspects were predicted based on both bioinformatics tools and published reports.”