Abstract
Legumes are cosmopolitan plants, and nodulating legumes are well known for their symbiotic nitrogen-fixing ability by rhizobia-legume interaction. Biological nitrogen fixation in non-nodulating endemic legumes by associative or endophytic symbiotic bacteria requires much needed attention. Three non-nodulating legumes, namely, Humboldtia brunonis Wall., Kunstleria keralensis C.N. Mohanan and N.C. Nair, and Bauhinia phoenicea Wight and Arn., endemic to the Western Ghats regions of Karnataka state, were studied. Employed techniques of selective culture media to understand diazotrophic diversity inside the roots of these plants. The isolates that can grow in a nitrogenfree semisolid agar medium have been considered positive for nitrogen-fixing ability. nifH gene is taken as the marker gene to ascertain the nitrogen-fixing ability of the bacteria. The qualified bacterium in the previous steps is identified using 16s RNA sequencing and the Sanger sequence method. The results obtained showed B. phoenicea Wight and Arn. and H. brunonis Wall. as Caulobacter segnis, and in K. keralensis C.N. Mohanan & N.C. Nair, it is Caulobacter crescentus. The presence of the nifH gene is demonstrated through molecular methods. This work adds to the diverse works of Caulobacter as a successful plant growth-promoting endophyte even in the nitrogen-deficient, slopy soils of the Western Ghats.
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Claims 4
Claim · model-written summary
The endophyte isolated from Bauhinia phoenicea was identified as Caulobacter segnis.
- Taxon
- B. phoenicea Wight and Arn.e1
- Interactor
- Caulobacter segnisCaulobacter segnise1symbiont
- Outcome
- identifiede2
Verbatim
e1“The results obtained showed B. phoenicea Wight and Arn. and H. brunonis Wall. as Caulobacter segnis, and in K. keralensis C.N. Mohanan & N.C. Nair, it is Caulobacter crescentus”
e2“The qualified bacterium in the previous steps is identified using 16s RNA sequencing”
Claim · model-written summary
The endophyte isolated from Humboldtia brunonis was identified as Caulobacter segnis.
- Taxon
- H. brunonis Wall.
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Humboldtia brunonise1 - Interactor
- Caulobacter segnisCaulobacter segnise1symbiont
- Outcome
- identifiede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“The results obtained showed B. phoenicea Wight and Arn. and H. brunonis Wall. as Caulobacter segnis, and in K. keralensis C.N. Mohanan & N.C. Nair, it is Caulobacter crescentus”
e2“The qualified bacterium in the previous steps is identified using 16s RNA sequencing”
Claim · model-written summary
The endophyte isolated from Kunstleria keralensis was identified as Caulobacter crescentus.
- Taxon
- K. keralensis C.N. Mohanan & N.C. Nair
Kunstleria keralensise1 - Interactor
- Caulobacter crescentus
Caulobacter vibrioidese1symbiont - Outcome
- identifiede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The results obtained showed B. phoenicea Wight and Arn. and H. brunonis Wall. as Caulobacter segnis, and in K. keralensis C.N. Mohanan & N.C. Nair, it is Caulobacter crescentus”
e2“The qualified bacterium in the previous steps is identified using 16s RNA sequencing”
Claim · model-written summary
The nifH gene was detected in the endophytic bacteria of the non-nodulating endemic legumes by molecular methods.
Verbatim
t0“Occurrence of two endophytic associative nitrogen-fixing Caulobacter spp., from three non-nodulating endemic legumes based on nifH gene analysis”
e3“The presence of the nifH gene is demonstrated through molecular methods”