Abstract
Wheat cultivar C271 registered as PI 210904 in the USDA National Small Grains Collection was developed from Punjab Pakistan in 1953 and it confers adult plant resistance (APR) to stripe rust both in the United States and China for many years. In the present study, we dissected the genetic basis of stripe rust resistance on 229 F2:3 populations produced by crossing Jinmai 79 and C271 in the fields of Yangling and Jiangyou. Bulked segregant analysis coupled with wheat 660K SNP array placed the majority of SNPs differences on chromosome arm 3BS. After using allele-specific quantitative PCR based genotyping assay (AQP) to confirm the SNPs, a linkage map was constructed and a major locus was detected across all environments based on IciMapping v4.1 software. The QTL, designated as YrC271, was flanked by SNP markers AX-109001377 and AX-111087256 with a genetic interval of 1.9 cM corresponding to a physical distance of 1.9 Mb in RefSeq v.1.0 (positions 6.1-8.0 Mb). Comparative genomics analysis was performed to detect the collinear genomic regions of different hexaploid wheat accessions (Triticum aestivum), T. dicoccoides, and T. turgidum. More than 340 SNPs in the physical region were extracted for haplotype analysis in a panel of over 1484 worldwide common wheat accessions, and five major haplotypes (Hap1, Hap2, Hap3, Hap4, and Hap5) were identified. And the favorable haplotype Hap1 was highly associated with stripe rust resistance. YrC271 appeared to be similar to YrC271 based on comparison of relative distance, stripe rust responses, and pedigree analyses, but allelism tests, cloning or precise phenotypic comparisons would be needed for confirmation. The YrC271 region provided the opportunity for further map-based cloning and haplotypes analysis enabled pyramiding favorable alleles into commercial cultivars by marker-assisted selection.
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Claims 4
Claim · model-written summary
A major stripe rust resistance locus was detected across environments.
- Taxon
- Wheat
Triticum aestivumt0 - Interactor
- stripe rustt0pathogen
- Gene
- major locuse0
- Outcome
- major locuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a major locus was detected across all environments”
t0“wheat stripe rust”
Claim · model-written summary
YrC271 was mapped between two SNP markers over a 1.9 cM interval.
- Taxon
- WheatTriticum aestivumt0
- Gene
- YrC271e0
- Outcome
- genetic intervale0
- Value
- 1.9 cMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The QTL, designated as YrC271, was flanked by SNP markers AX-109001377 and AX-111087256 with a genetic interval of 1.9 cM corresponding to a physical distance of 1.9 Mb”
t0“wheat stripe rust”
Claim · model-written summary
Five major haplotypes were identified in common wheat accessions.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- five major haplotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“five major haplotypes (Hap1, Hap2, Hap3, Hap4, and Hap5) were identified”
t0“wheat stripe rust”
Claim · model-written summary
Hap1 was highly associated with stripe rust resistance.
- Taxon
- WheatTriticum aestivumt0
- Material
- Hap1e0
- Interactor
- stripe ruste0pathogen
- Outcome
- stripe rust resistancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the favorable haplotype Hap1 was highly associated with stripe rust resistance”
t0“wheat stripe rust”