Abstract
Aim of the investigations is to carry out molecular-genetic study of sugar beet haploid plants-regenerants obtained by direct regeneration under in vitro culture. Results of these sugar beet lines’ molecular evaluation according to five microsattelite loci (SSR-markers – Unigenes: 24552, 14805, 16898, 22373 and 27833) and three minisattelites (TRs). As a result of the studies, experimental data characterizing initial sugar beet lines according to these molecular-genetic markers and allowing their clear differentiation have been obtained. Genetical distances (Euclidean) between the studied genotypes have been determined. The obtained sugar beet microclones have been clustered, and unique DNA-profiles for these breeding individuals have been made. From the results of the conducted molecular-genetic analysis of haploid plants-regenerants, it has been determined that DNA-markers of sugar beet mitochondrial genome belonging to the family of TRs minisatellites (TR1 and TR3) allow high-reliable identification of microclonal plants as MS and O-type forms. It has been revealed that plants-pollinators (sterility maintainers) of O-type are characterized by presence of DNA-amplicons: 700 bp in length when using the primer TR1 and 500 bp in length when using the primer TR3. The fragments of 400 bp are typical of MS plants-regenerants.
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Claims 8
Claim · model-written summary
Molecular marker data clearly differentiated the initial sugar beet lines.
- Taxon
- sugar beet
Beta vulgaris subsp. vulgarise0 - Outcome
- clear differentiatione0
- Comparator
- initial sugar beet linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“experimental data characterizing initial sugar beet lines according to these molecular-genetic markers and allowing their clear differentiation have been obtained”
Claim · model-written summary
Euclidean genetic distances were determined among the studied genotypes.
- Taxon
- SUGAR BEETBeta vulgaris subsp. vulgarist0
- Outcome
- Genetical distances (Euclidean)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUGAR BEET”
e0“Genetical distances (Euclidean) between the studied genotypes have been determined.”
Claim · model-written summary
Sugar beet microclones were clustered.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarise0
- Outcome
- microclones have been clusterede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The obtained sugar beet microclones have been clustered”
Claim · model-written summary
Unique DNA profiles were made for breeding individuals.
- Taxon
- SUGAR BEETBeta vulgaris subsp. vulgarist0
- Outcome
- unique DNA-profilese0
- Comparator
- these breeding individualse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUGAR BEET”
e0“unique DNA-profiles for these breeding individuals have been made”
Claim · model-written summary
TR1 and TR3 mitochondrial markers enabled reliable identification of microclones as MS and O type forms.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarise0
- Gene
- TR1 and TR3e0
- Outcome
- high-reliable identification of microclonal plants as MS and O-type formse0
- Comparator
- MS and O-type formse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA-markers of sugar beet mitochondrial genome belonging to the family of TRs minisatellites (TR1 and TR3) allow high-reliable identification of microclonal plants as MS and O-type forms”
Claim · model-written summary
O type sterility maintainers had a 700 bp TR1 amplicon.
- Taxon
- SUGAR BEETBeta vulgaris subsp. vulgarist0
- Material
- O-typee0
- Gene
- TR1e0
- Outcome
- presence of DNA-ampliconse0
- Value
- 700 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUGAR BEET”
e0“plants-pollinators (sterility maintainers) of O-type are characterized by presence of DNA-amplicons: 700 bp in length when using the primer TR1”
Claim · model-written summary
O type sterility maintainers had a 500 bp TR3 amplicon.
- Taxon
- SUGAR BEETBeta vulgaris subsp. vulgarist0
- Material
- O-typee0
- Gene
- TR3e0
- Outcome
- presence of DNA-ampliconse0
- Value
- 500 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUGAR BEET”
e0“plants-pollinators (sterility maintainers) of O-type are characterized by presence of DNA-amplicons: 700 bp in length when using the primer TR1 and 500 bp in length when using the primer TR3”
Claim · model-written summary
MS regenerants typically had 400 bp fragments.
- Taxon
- SUGAR BEETBeta vulgaris subsp. vulgarist0
- Material
- MS plants-regenerantse0
- Outcome
- fragmentse0
- Value
- 400 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“SUGAR BEET”
e0“The fragments of 400 bp are typical of MS plants-regenerants.”