Abstract
Despite a much later inception of somatic embryogenesis (SE) propagation protocols for gymnosperms than for angiosperm species, SE is becoming increasingly important due to its applications for commercial forestry. For many conifers, there are however still major bottlenecks in the SE plant production process limiting the use of SE for forestry operations, Christmas tree production and research projects. In the present case study, the effects on plant growth from different cultural factors applied during the SE developmental process were studied in two conifer species of high value for Christmas tree production. Seven clones of Abies nordmanniana and two clones of Abies bornmuelleriana were included in the study. Accumulated effects from cultural treatments were recorded from the start of germination of mature embryos of different quality scores through development into plants in the third growing period. Experimental factors of the cultural treatments included were: germination temperature, germination time, light conditions, survival ex vitro and traits for plant growth and vitality. The results reveal that most of the studied experimental factors influenced plant growth during the first three years however their relative importance was different. Plant survival rate at end of the nursery stage was strongly impacted by germination temperature (p<0.001), initial embryo score (p=0.007), clone (p<0.001) and to a lesser extend week of germination (p=0.017). This case-study highlights and quantifies the strong interrelation between the developmental steps of somatic embryogenesis and show the importance of considering all cultural steps when optimizing SE plant production protocols.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Germination temperature strongly impacted plant survival rate at the end of the nursery stage (p<0.001).
- Taxon
- Abies nordmanniana and Abies bornmuelleriana
Abies nordmanniana
Abies nordmanniana var. bornmuellerianat0 - Treatment
- germination temperaturee2
- Outcome
- Plant survival ratee2
- Qualifier
- p<0.001e2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Plant survival rate at end of the nursery stage was strongly impacted by germination temperature (p<0.001)”
t0“Abies nordmanniana and Abies bornmuelleriana”
Claim · model-written summary
Initial embryo score strongly impacted plant survival rate at the end of the nursery stage (p=0.007).
- Taxon
- Abies nordmanniana and Abies bornmuellerianaAbies nordmannianaAbies nordmanniana var. bornmuellerianat0
- Treatment
- initial embryo scoree2
- Outcome
- Plant survival ratee2
- Qualifier
- p=0.007e2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Plant survival rate at end of the nursery stage was strongly impacted by germination temperature (p<0.001), initial embryo score (p=0.007)”
t0“Abies nordmanniana and Abies bornmuelleriana”
Claim · model-written summary
Clone strongly impacted plant survival rate at the end of the nursery stage (p<0.001).
- Taxon
- Abies nordmanniana and Abies bornmuellerianaAbies nordmannianaAbies nordmanniana var. bornmuellerianat0
- Material
- clonee2
- Outcome
- Plant survival ratee2
- Qualifier
- p<0.001e2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Plant survival rate at end of the nursery stage was strongly impacted by germination temperature (p<0.001), initial embryo score (p=0.007), clone (p<0.001)”
t0“Abies nordmanniana and Abies bornmuelleriana”
Claim · model-written summary
Week of germination affected plant survival rate to a lesser extent (p=0.017).
- Taxon
- Abies nordmanniana and Abies bornmuellerianaAbies nordmannianaAbies nordmanniana var. bornmuellerianat0
- Treatment
- week of germinatione2
- Outcome
- Plant survival ratee2
- Qualifier
- p=0.017e2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Plant survival rate at end of the nursery stage was strongly impacted by germination temperature (p<0.001), initial embryo score (p=0.007), clone (p<0.001) and to a lesser extend week of germination (p=0.017)”
t0“Abies nordmanniana and Abies bornmuelleriana”
Claim · model-written summary
Most of the studied experimental factors influenced plant growth during the first three years, with different relative importance.
- Taxon
- Abies nordmanniana and Abies bornmuellerianaAbies nordmannianaAbies nordmanniana var. bornmuellerianat0
- Treatment
- studied experimental factorse0
- Outcome
- plant growthe0
- Qualifier
- during the first three yearse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results reveal that most of the studied experimental factors influenced plant growth during the first three years however their relative importance was different”
t0“Abies nordmanniana and Abies bornmuelleriana”
Claim · model-written summary
Optimizing somatic embryogenesis plant production protocols requires considering all cultural steps.
- Taxon
- Abies nordmanniana and Abies bornmuellerianaAbies nordmannianaAbies nordmanniana var. bornmuellerianat0
- Treatment
- considering all cultural stepse3
- Outcome
- SE plant production protocolse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“show the importance of considering all cultural steps when optimizing SE plant production protocols”
t0“Abies nordmanniana and Abies bornmuelleriana”