Abstract
BACKGROUND: Process and function that underlie the assembly of a rhizosphere microbial community may be strongly linked to the maintenance of plant health. However, their assembly processes and functional changes in the deterioration of soilborne disease remain unclear. Here, we investigated features of rhizosphere microbiomes related to Fusarium wilt disease and assessed their assembly by comparison pair of diseased/healthy sequencing data. The untargeted metabolomics was employed to explore potential community assembly drivers, and shotgun metagenome sequencing was used to reveal the mechanisms of metabolite-mediated process after soil conditioning. RESULTS: Results showed the deterministic assembly process associated with diseased rhizosphere microbiomes, and this process was significantly correlated to five metabolites (tocopherol acetate, citrulline, galactitol, octadecylglycerol, and behenic acid). Application of the metabolites resulted in a deterministic assembly of microbiome with the high morbidity of watermelon. Furthermore, metabolite conditioning was found to weaken the function of autotoxin degradation undertaken by specific bacterial group (Bradyrhizobium, Streptomyces, Variovorax, Pseudomonas, and Sphingomonas) while promoting the metabolism of small-molecule sugars and acids initiated from another bacterial group (Anaeromyxobacter, Bdellovibrio, Conexibacter, Flavobacterium, and Gemmatimonas). Video Abstract CONCLUSION: These findings strongly suggest that shifts in a metabolite-mediated microbial community assembly process underpin the deterministic establishment of soilborne Fusarium wilt disease and reveal avenues for future research focusing on ameliorating crop loss due to this pathogen.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Diseased rhizosphere microbiomes showed deterministic assembly.
- Taxon
- watermelon
Citrullus lanatuse1 - Outcome
- deterministic assembly processe0
- Qualifier
- diseased rhizosphere microbiomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results showed the deterministic assembly process associated with diseased rhizosphere microbiomes”
e1“high morbidity of watermelon”
Claim · model-written summary
Deterministic assembly correlated significantly with five named metabolites.
- Taxon
- watermelonCitrullus lanatuse1
- Compound
- tocopherol acetate, citrulline, galactitol, octadecylglycerol, and behenic acide0
- Outcome
- this processe0
- Qualifier
- five metabolitese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this process was significantly correlated to five metabolites (tocopherol acetate, citrulline, galactitol, octadecylglycerol, and behenic acid)”
e1“high morbidity of watermelon”
Claim · model-written summary
Applying the metabolites yielded deterministic microbiome assembly.
- Taxon
- watermelonCitrullus lanatuse0
- Treatment
- Application of the metabolitese0
- Outcome
- deterministic assembly of microbiomee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of the metabolites resulted in a deterministic assembly of microbiome with the high morbidity of watermelon.”
e1“five metabolites (tocopherol acetate, citrulline, galactitol, octadecylglycerol, and behenic acid)”
Claim · model-written summary
Applying the metabolites was associated with high watermelon morbidity.
- Taxon
- watermelonCitrullus lanatuse0
- Treatment
- Application of the metabolitese0
- Outcome
- morbidity of watermelone0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of the metabolites resulted in a deterministic assembly of microbiome with the high morbidity of watermelon.”
Claim · model-written summary
Metabolite conditioning weakened autotoxin degradation by the named bacterial group.
- Taxon
- watermelonCitrullus lanatuse1
- Treatment
- metabolite conditioninge0
- Interactor
- Bradyrhizobium, Streptomyces, Variovorax, Pseudomonas, and Sphingomonas
Pseudomonas
Bradyrhizobium
Streptomyces
SphingomonasVariovoraxe0symbiont - Outcome
- function of autotoxin degradatione0
- Direction
- weakene0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metabolite conditioning was found to weaken the function of autotoxin degradation undertaken by specific bacterial group (Bradyrhizobium, Streptomyces, Variovorax, Pseudomonas, and Sphingomonas)”
e1“high morbidity of watermelon”
Claim · model-written summary
Metabolite conditioning promoted bacterial metabolism of small-molecule sugars and acids.
- Taxon
- watermelonCitrullus lanatuse2
- Treatment
- metabolite conditioninge1
- Interactor
- Anaeromyxobacter, Bdellovibrio, Conexibacter, Flavobacterium, and GemmatimonasFlavobacterium
BdellovibrioAnaeromyxobacter
Gemmatimonas
Conexibactere0symbiont - Outcome
- metabolism of small-molecule sugars and acidse0
- Direction
- promotinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoting the metabolism of small-molecule sugars and acids initiated from another bacterial group (Anaeromyxobacter, Bdellovibrio, Conexibacter, Flavobacterium, and Gemmatimonas)”
e1“metabolite conditioning was found to weaken”
e2“high morbidity of watermelon”