Paper · 2022 · The Journal Agriculture and Forestry

HOW ENSURE IN VITRO POLLEN GERMINATION OF CULTIVATED FLAX AND ITS WILD RELATIVES

Anatoliy SOROKA; Viktor LYAKH

5
claims
6
verbatim quotes
2
citations
1
research areas
✓
Open access · cc-by

Abstract

Until recently, flax was considered a crop whose pollen does not germinate well under artificial conditions. At the same time, rapid acquisition of information on pollen quality is important in many studies. Two linseed varieties and its three wild relatives with n = 15 were used as pollen sources. The basic artificial nutrient medium for pollen germination consisted of 200 mg/L boric acid and 200 mg/L calcium chloride. Experimental treatments additionally included polyethylene glycol of various molecular weights and sucrose. The media with polyethylene glycol 2000 in concentrations of 20-30 % ensured good pollen germination of cultivated flax, showing about 40% of pollen grains with normal pollen tubes. For wild species pollen, 20 % osmotic concentration was more preferable. The addition of sucrose to a medium with polyethylene glycol 2000 disadvantageously influenced pollen germination of cultivated flax and did not affect this indicator for wild species. When polyethylene glycol of higher molecular weight was used in a nutrient medium as an osmotic, the germinated pollen grains usually had burst pollen tubes or did not germinate at all.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Media with polyethylene glycol 2000 at 20-30 % ensured good pollen germination of cultivated flax, with about 40% of pollen grains showing normal pollen tubes.

Taxon
cultivated flaxLinum usitatissimume0
Treatment
polyethylene glycol 2000 in concentrations of 20-30 %e0
Outcome
pollen germinatione0
Direction
goode0
Value
about 40%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Linum usitatissimum
Linum usitatissimum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The media with polyethylene glycol 2000 in concentrations of 20-30 % ensured good pollen germination of cultivated flax, showing about 40% of pollen grains with normal pollen tubes”

✓ located verbatim in the source · abstract, characters 532–712 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

For wild flax species pollen, a 20 % osmotic concentration was more preferable.

Taxon
wild speciese0
Treatment
20 % osmotic concentratione0
Outcome
more preferablee0
Direction
more preferablee0

Not reportedComparator

Verbatim

e0

“For wild species pollen, 20 % osmotic concentration was more preferable”

✓ located verbatim in the source · abstract, characters 714–785 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Adding sucrose to a medium with polyethylene glycol 2000 disadvantageously influenced pollen germination of cultivated flax.

Taxon
cultivated flaxLinum usitatissimume0
Treatment
addition of sucrose to a medium with polyethylene glycol 2000e0
Outcome
pollen germinatione0
Direction
disadvantageously influencede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Linum usitatissimum
Linum usitatissimum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The addition of sucrose to a medium with polyethylene glycol 2000 disadvantageously influenced pollen germination of cultivated flax and did not affect this indicator for wild species”

✓ located verbatim in the source · abstract, characters 787–970 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Adding sucrose to a medium with polyethylene glycol 2000 did not affect pollen germination of wild flax species.

Taxon
wild speciese0
Treatment
addition of sucrose to a medium with polyethylene glycol 2000e0
Outcome
pollen germinatione0
Direction
did not affecte0

Not reportedComparator

Verbatim

e0

“The addition of sucrose to a medium with polyethylene glycol 2000 disadvantageously influenced pollen germination of cultivated flax and did not affect this indicator for wild species”

✓ located verbatim in the source · abstract, characters 787–970 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

With higher molecular weight polyethylene glycol as the osmotic in the medium, germinated pollen grains usually had burst pollen tubes or did not germinate at all.

Treatment
polyethylene glycol of higher molecular weighte0
Outcome
burst pollen tubes or did not germinate at alle0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Linum usitatissimum
Linum usitatissimum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“When polyethylene glycol of higher molecular weight was used in a nutrient medium as an osmotic, the germinated pollen grains usually had burst pollen tubes or did not germinate at all”

✓ located verbatim in the source · abstract, characters 972–1156 · show in text ↑
t0

“FLAX”

✓ located verbatim in the source · title, characters 53–57 · show in text ↑

poly(ethylene glycol) · growth · Linum usitatissimum (flax)Results agree

4 papers: 0 positive, 4 negative or no effect

3 papers in the same direction as this paper

  1. negative
  2. no effect
  3. negative
See all 4 papers →

in vitro · yield · Linum usitatissimum (flax)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.