Abstract
Until recently, flax was considered a crop whose pollen does not germinate well under artificial conditions. At the same time, rapid acquisition of information on pollen quality is important in many studies. Two linseed varieties and its three wild relatives with n = 15 were used as pollen sources. The basic artificial nutrient medium for pollen germination consisted of 200 mg/L boric acid and 200 mg/L calcium chloride. Experimental treatments additionally included polyethylene glycol of various molecular weights and sucrose. The media with polyethylene glycol 2000 in concentrations of 20-30 % ensured good pollen germination of cultivated flax, showing about 40% of pollen grains with normal pollen tubes. For wild species pollen, 20 % osmotic concentration was more preferable. The addition of sucrose to a medium with polyethylene glycol 2000 disadvantageously influenced pollen germination of cultivated flax and did not affect this indicator for wild species. When polyethylene glycol of higher molecular weight was used in a nutrient medium as an osmotic, the germinated pollen grains usually had burst pollen tubes or did not germinate at all.
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Claims 5
Claim · model-written summary
Media with polyethylene glycol 2000 at 20-30 % ensured good pollen germination of cultivated flax, with about 40% of pollen grains showing normal pollen tubes.
- Taxon
- cultivated flax
Linum usitatissimume0
- Treatment
- polyethylene glycol 2000 in concentrations of 20-30 %e0
- Outcome
- pollen germinatione0
- Direction
- goode0
- Value
- about 40%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The media with polyethylene glycol 2000 in concentrations of 20-30 % ensured good pollen germination of cultivated flax, showing about 40% of pollen grains with normal pollen tubes”
Claim · model-written summary
For wild flax species pollen, a 20 % osmotic concentration was more preferable.
- Taxon
- wild speciese0
- Treatment
- 20 % osmotic concentratione0
- Outcome
- more preferablee0
- Direction
- more preferablee0
Not reportedComparator
Verbatim
e0“For wild species pollen, 20 % osmotic concentration was more preferable”
Claim · model-written summary
Adding sucrose to a medium with polyethylene glycol 2000 disadvantageously influenced pollen germination of cultivated flax.
- Taxon
- cultivated flax
Linum usitatissimume0
- Treatment
- addition of sucrose to a medium with polyethylene glycol 2000e0
- Outcome
- pollen germinatione0
- Direction
- disadvantageously influencede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of sucrose to a medium with polyethylene glycol 2000 disadvantageously influenced pollen germination of cultivated flax and did not affect this indicator for wild species”
Claim · model-written summary
Adding sucrose to a medium with polyethylene glycol 2000 did not affect pollen germination of wild flax species.
- Taxon
- wild speciese0
- Treatment
- addition of sucrose to a medium with polyethylene glycol 2000e0
- Outcome
- pollen germinatione0
- Direction
- did not affecte0
Not reportedComparator
Verbatim
e0“The addition of sucrose to a medium with polyethylene glycol 2000 disadvantageously influenced pollen germination of cultivated flax and did not affect this indicator for wild species”
Claim · model-written summary
With higher molecular weight polyethylene glycol as the osmotic in the medium, germinated pollen grains usually had burst pollen tubes or did not germinate at all.
- Taxon
- FLAX
Linum usitatissimumt0
- Treatment
- polyethylene glycol of higher molecular weighte0
- Outcome
- burst pollen tubes or did not germinate at alle0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When polyethylene glycol of higher molecular weight was used in a nutrient medium as an osmotic, the germinated pollen grains usually had burst pollen tubes or did not germinate at all”
t0“FLAX”
The same questions in other papers
poly(ethylene glycol) · growth · Linum usitatissimum (flax)Results agree
3 papers in the same direction as this paper
- negative
- no effect
- negative
in vitro · yield · Linum usitatissimum (flax)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.