Abstract
Flax is grown worldwide for seed and fiber production. Linseed varieties differ in their oil composition and are used in pharmaceutical, food, feed, and industrial production. The field of application primarily depends on the content of linolenic (LIN) and linoleic (LIO) fatty acids. Inactivating mutations in the FAD3A and FAD3B genes lead to a decrease in the LIN content and an increase in the LIO content. For the identification of the three most common low-LIN mutations in flax varieties (G-to-A in exon 1 of FAD3A substituting tryptophan with a stop codon, C-to-T in exon 5 of FAD3A leading to arginine to a stop codon substitution, and C-to-T in exon 2 of FAD3B resulting in histidine to tyrosine substitution), three approaches were proposed: (1) targeted deep sequencing, (2) high resolution melting (HRM) analysis, (3) cleaved amplified polymorphic sequences (CAPS) markers. They were tested on more than a thousand flax samples of various types and showed promising results. The proposed approaches can be used in marker-assisted selection to choose parent pairs for crosses, separate heterogeneous varieties into biotypes, and select genotypes with desired homozygous alleles of the FAD3A and FAD3B genes at the early stages of breeding for the effective development of varieties with a particular LIN and LIO content, as well as in basic studies of the molecular mechanisms of fatty acid synthesis in flax seeds to select genotypes adequate to the tasks.
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Claims 6
Claim · model-written summary
Inactivating FAD3A and FAD3B mutations decrease LIN content.
- Taxon
- Flax
Linum usitatissimumt0 - Gene
- FAD3A and FAD3Be0
- Outcome
- LIN contente0
- Direction
- decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inactivating mutations in the FAD3A and FAD3B genes lead to a decrease in the LIN content and an increase in the LIO content”
t0“Development and Complex Application of Methods for the Identification of Mutations in the FAD3A and FAD3B Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil”
e1“three approaches were proposed: (1) targeted deep sequencing, (2) high resolution melting (HRM) analysis, (3) cleaved amplified polymorphic sequences (CAPS) markers”
Claim · model-written summary
Inactivating FAD3A and FAD3B mutations increase LIO content.
- Taxon
- FlaxLinum usitatissimumt0
- Gene
- FAD3A and FAD3Be0
- Outcome
- LIO contente0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inactivating mutations in the FAD3A and FAD3B genes lead to a decrease in the LIN content and an increase in the LIO content”
t0“Development and Complex Application of Methods for the Identification of Mutations in the FAD3A and FAD3B Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil”
e1“three approaches were proposed: (1) targeted deep sequencing, (2) high resolution melting (HRM) analysis, (3) cleaved amplified polymorphic sequences (CAPS) markers”
Claim · model-written summary
Targeted deep sequencing showed promising results for identifying low-LIN mutations across more than a thousand flax samples.
- Taxon
- flaxLinum usitatissimume0
- Treatment
- targeted deep sequencinge1
- Outcome
- promising resultse0
- Value
- more than a thousand flax samplese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They were tested on more than a thousand flax samples of various types and showed promising results”
t0“Development and Complex Application of Methods for the Identification of Mutations in the FAD3A and FAD3B Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil”
e1“three approaches were proposed: (1) targeted deep sequencing, (2) high resolution melting (HRM) analysis, (3) cleaved amplified polymorphic sequences (CAPS) markers”
Claim · model-written summary
High resolution melting analysis showed promising results for identifying low-LIN mutations across more than a thousand flax samples.
- Taxon
- flaxLinum usitatissimume0
- Treatment
- high resolution melting (HRM) analysise1
- Outcome
- promising resultse0
- Value
- more than a thousand flax samplese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They were tested on more than a thousand flax samples of various types and showed promising results”
t0“Development and Complex Application of Methods for the Identification of Mutations in the FAD3A and FAD3B Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil”
e1“three approaches were proposed: (1) targeted deep sequencing, (2) high resolution melting (HRM) analysis, (3) cleaved amplified polymorphic sequences (CAPS) markers”
Claim · model-written summary
CAPS markers showed promising results for identifying low-LIN mutations across more than a thousand flax samples.
- Taxon
- flaxLinum usitatissimume0
- Treatment
- cleaved amplified polymorphic sequences (CAPS) markerse1
- Outcome
- promising resultse0
- Value
- more than a thousand flax samplese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They were tested on more than a thousand flax samples of various types and showed promising results”
t0“Development and Complex Application of Methods for the Identification of Mutations in the FAD3A and FAD3B Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil”
e1“three approaches were proposed: (1) targeted deep sequencing, (2) high resolution melting (HRM) analysis, (3) cleaved amplified polymorphic sequences (CAPS) markers”
Claim · model-written summary
Authors propose the assays for selecting breeding parents, resolving heterogeneous varieties and selecting desired homozygotes early.
- Taxon
- FlaxLinum usitatissimumt0
- Outcome
- choose parent pairs for crosses, separate heterogeneous varieties into biotypes, and select genotypes with desired homozygous allelese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The proposed approaches can be used in marker-assisted selection to choose parent pairs for crosses, separate heterogeneous varieties into biotypes, and select genotypes with desired homozygous alleles of the FAD3A and FAD3B genes at the early stages of breeding”
t0“Development and Complex Application of Methods for the Identification of Mutations in the FAD3A and FAD3B Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil”
e1“three approaches were proposed: (1) targeted deep sequencing, (2) high resolution melting (HRM) analysis, (3) cleaved amplified polymorphic sequences (CAPS) markers”