Paper · 2022 · Forestry Engineering Journal

Effect of different led spectrum regimens on growth and development of Betula pubescens Ehrh. and Rubus idaeus L. in culture in vitro

Petr Evlakov; Tatyana Grodeckaya; Olga Fedorova; et al.

6
claims
13
verbatim quotes
5
citations
2
research areas
✓
Open access

Abstract

Light-emitting diodes (LEDs) have shown high efficiency in growing plants both in vivo in greenhouses and in vitro, including clonal micropropagation. The purpose of this study was to analyze the effect of the spectral composition of LED irradiators with different proportions of red (RL) and blue (BL) light on the morphogenesis of microplants of the remontant form of common raspberry (Rubus idaeus L.) cv. Hercules and the selection valuable cultivar of downy birch (Betula pubescens Ehrh.), which is the object of a unified genetic breeding complex (UGBC), previously selected on the basis of drought resistance. In the variant 1, the RL/BL ratio was 80/20%, in the variant 2 it was 70/30%, in the control it was 50/50%. The LED in variant 1 contributed to the greatest increase in morphometric and anatomical characteristics in raspberry microplants, increasing the height of shoots, the number of leaves, stomata density, the height of the leaf epidermis and mesophyll. At the same time, an increase in the proportion of RL/BL led to an increase in the leaf area, leaf surface, and stomatal density in birch microclones; however, the anatomical characteristics of the leaf indicate a decrease in the height of epidermal cells and the size of mesophyll cells. Thus, the LED of option 1 can be recommended for use in clonal micropropagation of raspberries in greenhouses, to optimize growth processes and obtain healthy, normally formed plants, while birch requires additional selection of optimal spectral illumination conditions.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The 80/20 RL/BL LED variant increased shoot height, leaf number and stomata density in raspberry microplants relative to the control.

Taxon
Rubus idaeus L.Rubus idaeuse2
Material
cv. Herculese2
Treatment
the RL/BL ratio was 80/20%e3
Outcome
height of shoots, the number of leaves, stomata densitye1
Direction
increasinge1
Comparator
controle3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rubus idaeus
Rubus idaeus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“The LED in variant 1 contributed to the greatest increase in morphometric and anatomical characteristics in raspberry microplants, increasing the height of shoots, the number of leaves, stomata density, the height of the leaf epidermis and mesophyll”

✓ located verbatim in the source · abstract, characters 725–974 · show in text ↑
e2

“common raspberry (Rubus idaeus L.) cv. Hercules and the selection valuable cultivar of downy birch (Betula pubescens Ehrh.)”

✓ located verbatim in the source · abstract, characters 370–493 · show in text ↑
e3

“In the variant 1, the RL/BL ratio was 80/20%, in the variant 2 it was 70/30%, in the control it was 50/50%”

✓ located verbatim in the source · abstract, characters 617–723 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The 80/20 RL/BL LED variant increased the height of the leaf epidermis and mesophyll in raspberry microplants relative to the control.

Taxon
Rubus idaeus L.Rubus idaeuse2
Material
cv. Herculese2
Treatment
the RL/BL ratio was 80/20%e3
Outcome
the height of the leaf epidermis and mesophylle1
Direction
increasinge1
Comparator
controle3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rubus idaeus
Rubus idaeus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“The LED in variant 1 contributed to the greatest increase in morphometric and anatomical characteristics in raspberry microplants, increasing the height of shoots, the number of leaves, stomata density, the height of the leaf epidermis and mesophyll”

✓ located verbatim in the source · abstract, characters 725–974 · show in text ↑
e2

“common raspberry (Rubus idaeus L.) cv. Hercules and the selection valuable cultivar of downy birch (Betula pubescens Ehrh.)”

✓ located verbatim in the source · abstract, characters 370–493 · show in text ↑
e3

“In the variant 1, the RL/BL ratio was 80/20%, in the variant 2 it was 70/30%, in the control it was 50/50%”

✓ located verbatim in the source · abstract, characters 617–723 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Increasing the RL/BL proportion increased leaf area, leaf surface and stomatal density in birch microclones.

Taxon
Betula pubescens Ehrh.Betula pubescense2
Treatment
the proportion of RL/BLe4
Outcome
leaf area, leaf surface, and stomatal densitye4
Direction
increasee4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Betula pubescens
Betula pubescens · TaxonElfo del bosque · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“an increase in the proportion of RL/BL led to an increase in the leaf area, leaf surface, and stomatal density in birch microclones”

✓ located verbatim in the source · abstract, characters 994–1125 · show in text ↑
e2

“common raspberry (Rubus idaeus L.) cv. Hercules and the selection valuable cultivar of downy birch (Betula pubescens Ehrh.)”

✓ located verbatim in the source · abstract, characters 370–493 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Increasing the RL/BL proportion decreased the height of epidermal cells and the size of mesophyll cells in birch leaves.

Taxon
Betula pubescens Ehrh.Betula pubescense2
Treatment
the proportion of RL/BLe4
Outcome
height of epidermal cells and the size of mesophyll cellse5
Direction
decreasee5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Betula pubescens
Betula pubescens · TaxonElfo del bosque · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e5

“the anatomical characteristics of the leaf indicate a decrease in the height of epidermal cells and the size of mesophyll cells”

✓ located verbatim in the source · abstract, characters 1136–1263 · show in text ↑
e2

“common raspberry (Rubus idaeus L.) cv. Hercules and the selection valuable cultivar of downy birch (Betula pubescens Ehrh.)”

✓ located verbatim in the source · abstract, characters 370–493 · show in text ↑
e4

“an increase in the proportion of RL/BL led to an increase in the leaf area, leaf surface, and stomatal density in birch microclones”

✓ located verbatim in the source · abstract, characters 994–1125 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The 80/20 RL/BL LED option can be recommended for use in clonal micropropagation of raspberries in greenhouses.

Taxon
raspberriese6
Outcome
use in clonal micropropagatione6

Verbatim

e6

“the LED of option 1 can be recommended for use in clonal micropropagation of raspberries in greenhouses, to optimize growth processes and obtain healthy, normally formed plants”

✓ located verbatim in the source · abstract, characters 1271–1447 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Birch requires additional selection of optimal spectral illumination conditions.

Taxon
birche7
Outcome
additional selection of optimal spectral illumination conditionse7

Verbatim

e7

“while birch requires additional selection of optimal spectral illumination conditions”

✓ located verbatim in the source · abstract, characters 1449–1534 · show in text ↑

light · growth · Rubus idaeus (red raspberry)Positive and negative results

12 papers: 7 positive, 5 negative or no effect

6 papers in the same direction as this paper · 5 in the opposite direction

  1. positive
  2. no effect
  3. no effect

8 papers more in this group

See all 12 papers →

light · physiology: gas exchange · Rubus idaeus (red raspberry)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.