Abstract
The aim of this work is development and validation of a spectrophotometric technique for quantification of total flavonoids in terms of rutin in potentially medicinal plant Anthemis tinctoria herb. According to the literature, Anthemis tinctoria is used in folk medicine as a hepatoprotective, hemostatic and means, and its extracts in the experiment exhibit antimicrobial, cytostatic and antioxidant activities. In this work, we used sample of Anthemis tinctoria herb harvested in the summer of 2021 during the period of mass flowering in vicinity of Perm city (valley of the Vasilievka river, Adishchevo railway station). The raw materials were dried at room temperature, with sufficient ventilation, without direct exposure to sunlight, stored separately from other types of raw materials. According to the experiments the optimal conditions for determining of total flavonoids: grinding the raw material to a size passing through a sieve with a diameter of 1 mm, the optimal concentration of ethyl alcohol extractant is 50%, the analytical wavelength is 410 nm, the extraction time in a boiling water bath is 45 minutes, the ratio of extraction and complexing agent - 2% alcohol solution of aluminum chloride - 2:5. The technique was validated in accordance with the requirements of State Pharmacopeia of Russia 14th edition, the relative error of determination with a reliable probability of 95% was 4.00%. The technique can be used in further work to conduct studies of biological activity.
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Claims 6
Claim · model-written summary
The validated spectrophotometric technique for total flavonoids in Anthemis tinctoria herb had a relative error of 4.00%.
- Taxon
- Anthemis tinctoria
Cota tinctoriae0 - Outcome
- relative error of determinatione1
- Value
- 4.00%e1
- Qualifier
- with a reliable probability of 95%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in potentially medicinal plant Anthemis tinctoria herb”
e1“the relative error of determination with a reliable probability of 95% was 4.00%”
Claim · model-written summary
The optimal concentration of ethyl alcohol extractant for total flavonoids in Anthemis tinctoria herb was 50%.
- Taxon
- Anthemis tinctoriaCota tinctoriae0
- Outcome
- optimal concentration of ethyl alcohol extractante2
- Value
- 50%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in potentially medicinal plant Anthemis tinctoria herb”
e2“the optimal concentration of ethyl alcohol extractant is 50%”
Claim · model-written summary
The optimal analytical wavelength for total flavonoids in Anthemis tinctoria herb was 410 nm.
- Taxon
- Anthemis tinctoriaCota tinctoriae0
- Outcome
- analytical wavelengthe3
- Value
- 410 nme3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in potentially medicinal plant Anthemis tinctoria herb”
e3“the analytical wavelength is 410 nm”
Claim · model-written summary
The optimal extraction time in a boiling water bath for total flavonoids in Anthemis tinctoria herb was 45 minutes.
- Taxon
- Anthemis tinctoriaCota tinctoriae0
- Outcome
- extraction timee4
- Value
- 45 minutese4
- Qualifier
- in a boiling water bathe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in potentially medicinal plant Anthemis tinctoria herb”
e4“the extraction time in a boiling water bath is 45 minutes”
Claim · model-written summary
The optimal ratio of extraction and complexing agent for total flavonoids in Anthemis tinctoria herb was 2:5.
- Taxon
- Anthemis tinctoriaCota tinctoriae0
- Outcome
- ratio of extraction and complexing agente5
- Value
- 2:5e5
- Qualifier
- 2% alcohol solution of aluminum chloridee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in potentially medicinal plant Anthemis tinctoria herb”
e5“the ratio of extraction and complexing agent - 2% alcohol solution of aluminum chloride - 2:5”
Claim · model-written summary
The optimal grinding of Anthemis tinctoria herb raw material for total flavonoids was a sieve of 1 mm.
- Taxon
- Anthemis tinctoriaCota tinctoriae0
- Outcome
- grinding the raw materiale6
- Value
- 1 mme6
- Qualifier
- to a size passing through a sievee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in potentially medicinal plant Anthemis tinctoria herb”
e6“grinding the raw material to a size passing through a sieve with a diameter of 1 mm”