Abstract
This study aimed to investigate the existence of wheat seed gall nematode Anguina tritici in the silos and some wheat fileds in Duhok province / Kurdistan Region - Iraq.,and diagnose four new isolates of this nematode on wheat and one isolate on barley collected from different loactions in Iraq.The results indiacted that wheat impurities samples were infested with seed galls by 66 % as the highest percentager in silo of Faidia during 2020, while the lowest (6%) recoded in the silo of Zakho during the same year.The highest disease incidence was recorded in the wheat fields of Akre by 34.6%,while the lowest (2%) in the wheat fields of Semel. Molecular identification results revealed that the bands of amplified DNA products of all nematode isolates were visualized by agarose gel electrophoresis in the same position ("~" up to 750 bp).Sequencing of the partial gene 5.8S rRNA gene confirmed that all nematode isolates belong to seed gall nematode A.tritici, also a description of their accession number by blast program showed the same percentage identitiy (100 %), and their comparision by a DNA Dot Plot emphasized that they are genetically similar. Results of phylogenetic tree analysis showed grouping of nematode isolates with each other and with the other two isolates of the same species from Iraq,whilst nucleotide variations increased with other nematode species of the same genus,and increased more with other nematode species of the same family (Anguinidae).
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Claims 10
Claim · model-written summary
Wheat impurities in Faidia silo had 66% seed gall infestation, the highest reported.
- Taxon
- wheat
Triticum aestivume0 - Interactor
- seed gallse0pest
- Outcome
- infested with seed gallse0
- Value
- 66 %e0
- Setting
- silo of Faidiae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wheat impurities samples were infested with seed galls by 66 % as the highest percentager in silo of Faidia during 2020”
Claim · model-written summary
Wheat impurities in Zakho silo had the lowest infestation at 6%.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- loweste0
- Value
- 6%e0
- Setting
- silo of Zakhoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“WHEAT AND BARLEY”
e0“the lowest (6%) recoded in the silo of Zakho during the same year”
Claim · model-written summary
Wheat disease incidence was highest in Akre fields at 34.6%.
- Taxon
- wheatTriticum aestivume0
- Outcome
- disease incidencee0
- Value
- 34.6%e0
- Setting
- Akree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest disease incidence was recorded in the wheat fields of Akre by 34.6%”
Claim · model-written summary
Wheat disease incidence was lowest in Semel fields at 2%.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- wheat fields of Semele0
- Value
- 2%e0
- Setting
- Semele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“WHEAT AND BARLEY”
e0“the lowest (2%) in the wheat fields of Semel”
Claim · model-written summary
All nematode isolates showed DNA bands at approximately 750 bp.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- bands of amplified DNA products of all nematode isolatese0
- Value
- 750 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“WHEAT AND BARLEY”
e0“bands of amplified DNA products of all nematode isolates were visualized by agarose gel electrophoresis in the same position ("~" up to 750 bp)”
Claim · model-written summary
5.8S rRNA sequencing identified all isolates as A. tritici.
- Taxon
- WHEATTriticum aestivumt0
- Interactor
- A.tritici
.jpg?width=60)
Anguina triticie0pest - Gene
- 5.8S rRNA genee0
- Outcome
- all nematode isolates belong to seed gall nematode A.triticie0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“WHEAT AND BARLEY”
e0“Sequencing of the partial gene 5.8S rRNA gene confirmed that all nematode isolates belong to seed gall nematode A.tritici”
Claim · model-written summary
The isolates had 100% sequence identity and were genetically similar by DNA Dot Plot.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- genetically similare0
- Value
- 100 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“WHEAT AND BARLEY”
e0“same percentage identitiy (100 %), and their comparision by a DNA Dot Plot emphasized that they are genetically similar”
Claim · model-written summary
Phylogenetic analysis grouped the isolates with each other and two other Iraqi isolates.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- grouping of nematode isolatese0
- Setting
- Iraqe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“WHEAT AND BARLEY”
e0“grouping of nematode isolates with each other and with the other two isolates of the same species from Iraq”
Claim · model-written summary
Nucleotide variation increased relative to other nematode species of the same genus.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- nucleotide variationse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“WHEAT AND BARLEY”
e0“nucleotide variations increased with other nematode species of the same genus”
Claim · model-written summary
Nucleotide variation increased more relative to other species of Anguinidae.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- increased moree0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“WHEAT AND BARLEY”
e0“increased more with other nematode species of the same family (Anguinidae)”