Paper · 2023 · PLoS ONE

Comparative study on chloroplast genomes of three Hansenia forbesii varieties (Apiaceae)

Chenghao Zhu; Yuan Jiang; Yu Bai; et al.

7
claims
9
verbatim quotes
1
citations
4
research areas
✓
Open access · cc-by

Abstract

To find the gene hypervariable regions of three varieties of Hansenia forbesii H. Boissieu and determine their phylogenetic relationship, the chloroplast (cp) genome of these three varieties were firstly sequencing by the Illumina hiseq platform. In this study, we assembled the complete cp genome sequences of Hansenia forbesii LQ (156,954 bp), H. forbesii QX (157,181 bp), H. forbesii WQ (156,975 bp). They all contained 84 protein-coding genes, 37 tRNAs, and 8 rRNAs. The hypervariable regions between three cp genomes were atpF-atpH, petD, and rps15-ycf1. Phylogenetic analysis showed that H. forbesii LQ and H. forbesii WQ were closely related, followed by H. forbesii QX. This study showed that the three varieties of H. forbesii could be identified by the complete cp genome and specific DNA barcode (trnC-GCA-petN) and provided a new idea for germplasm identification of similar cultivated varieties.

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

Complete cp genome of Hansenia forbesii LQ is 156,954 bp.

Taxon
Hansenia forbesiiHansenia forbesiie1
Material
LQe1
Outcome
complete cp genome sequencese1
Value
156,954 bpe1

Verbatim

e1

“we assembled the complete cp genome sequences of Hansenia forbesii LQ (156,954 bp), H. forbesii QX (157,181 bp), H. forbesii WQ (156,975 bp)”

✓ located verbatim in the source · abstract, characters 262–402 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Complete cp genome of Hansenia forbesii QX is 157,181 bp.

Taxon
Hansenia forbesiiHansenia forbesiie1
Material
QXe1
Outcome
complete cp genome sequencese1
Value
157,181 bpe1

Verbatim

e1

“we assembled the complete cp genome sequences of Hansenia forbesii LQ (156,954 bp), H. forbesii QX (157,181 bp), H. forbesii WQ (156,975 bp)”

✓ located verbatim in the source · abstract, characters 262–402 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Complete cp genome of Hansenia forbesii WQ is 156,975 bp.

Taxon
Hansenia forbesiiHansenia forbesiie1
Material
WQe1
Outcome
complete cp genome sequencese1
Value
156,975 bpe1

Verbatim

e1

“we assembled the complete cp genome sequences of Hansenia forbesii LQ (156,954 bp), H. forbesii QX (157,181 bp), H. forbesii WQ (156,975 bp)”

✓ located verbatim in the source · abstract, characters 262–402 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The three cp genomes each contain 84 protein-coding genes, 37 tRNAs and 8 rRNAs.

Taxon
Hansenia forbesiiHansenia forbesiie1
Outcome
protein-coding genese2
Value
84 protein-coding genes, 37 tRNAs, and 8 rRNAse2

Verbatim

e1

“we assembled the complete cp genome sequences of Hansenia forbesii LQ (156,954 bp), H. forbesii QX (157,181 bp), H. forbesii WQ (156,975 bp)”

✓ located verbatim in the source · abstract, characters 262–402 · show in text ↑
e2

“They all contained 84 protein-coding genes, 37 tRNAs, and 8 rRNAs”

✓ located verbatim in the source · abstract, characters 404–469 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Hypervariable regions between the three cp genomes are atpF-atpH, petD and rps15-ycf1.

Taxon
Hansenia forbesiiHansenia forbesiie1
Gene
atpF-atpH, petD, and rps15-ycf1e3
Outcome
hypervariable regionse3

Verbatim

e1

“we assembled the complete cp genome sequences of Hansenia forbesii LQ (156,954 bp), H. forbesii QX (157,181 bp), H. forbesii WQ (156,975 bp)”

✓ located verbatim in the source · abstract, characters 262–402 · show in text ↑
e3

“The hypervariable regions between three cp genomes were atpF-atpH, petD, and rps15-ycf1”

✓ located verbatim in the source · abstract, characters 471–558 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

H. forbesii LQ and WQ are closely related, followed by QX.

Taxon
H. forbesiiHansenia forbesiie4
Material
H. forbesii LQe4
Outcome
closely relatede4
Qualifier
followed by H. forbesii QXe4

Verbatim

e4

“Phylogenetic analysis showed that H. forbesii LQ and H. forbesii WQ were closely related, followed by H. forbesii QX”

✓ located verbatim in the source · abstract, characters 560–676 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The three varieties could be identified by the complete cp genome and the DNA barcode trnC-GCA-petN.

Taxon
H. forbesiiHansenia forbesiie5
Gene
trnC-GCA-petNe5
Outcome
could be identifiede5
Comparator
complete cp genomee5

Verbatim

e5

“the three varieties of H. forbesii could be identified by the complete cp genome and specific DNA barcode (trnC-GCA-petN)”

✓ located verbatim in the source · abstract, characters 701–822 · show in text ↑