Abstract
Research carried out in 2021 at the Jeser Al-Musayyab Company, Baghdad, Iraq, determined the molecular variations among 10 inbred lines of maize (Zea mays L.), for use in the future production of promising F1 hybrids. DNA sequencing random polymorphism technology using Inter Simple Sequence Repeats (ISSR) with five primers (UBC 834, UBC 835, UBC 856, UBC 866, and UBC 897) assessed the genetic variations among the 10 inbred lines of maize. All five primers produced 29 bands, averaging 5.8 strips each primer. The four primers, UBC 834, UBC 835, UBC 866, and UBC 897, had produced the same and highest number of proliferators with molecular weights ranging from 100 to 2000 bp. The primer UBC 856 showed the lowest number, with a molecular weight of 100–500 bp. The total number of polymorphs was 24, with a percentage of 82.4%, with the lowest (80%) polymorphs recorded in the primer UBC 856. The least discriminatory efficiency and ability values were 17.24% and 16.66%, respectively. Relying on binary data and genetic divergence using the UPGMA method to create a genetic kinship tree, the maize lines were separated into two groups, i.e., A, B, secondary, and sub-secondary groups. The highest genetic similarity (with the lowest genetic divergence) was estimated according to the Nei and Li coefficient of 0.23 between the two lines HS and S-10. The presented findings can provide further guidance and help analyze the genetic diversity among the maize populations.
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Claims 9
Claim · model-written summary
Five ISSR primers produced 29 bands, averaging 5.8 per primer.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All five primers produced 29 bands, averaging 5.8 strips each primer.”
t0“MAIZE”
Claim · model-written summary
Four primers produced the highest number of proliferators.
- Material
- UBC 834, UBC 835, UBC 866, and UBC 897e0
- Outcome
- number of proliferatorse0
- Direction
- higheste0
- Value
- 100 to 2000 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The four primers, UBC 834, UBC 835, UBC 866, and UBC 897, had produced the same and highest number of proliferators with molecular weights ranging from 100 to 2000 bp.”
t0“MAIZE”
Claim · model-written summary
UBC 856 produced the lowest number.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The primer UBC 856 showed the lowest number, with a molecular weight of 100–500 bp.”
t0“MAIZE”
Claim · model-written summary
The primers yielded 24 polymorphs, 82.4%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The total number of polymorphs was 24, with a percentage of 82.4%”
t0“MAIZE”
Claim · model-written summary
UBC 856 had the lowest polymorphism, 80%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lowest (80%) polymorphs recorded in the primer UBC 856.”
t0“MAIZE”
Claim · model-written summary
Least discriminatory efficiency was 17.24%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The least discriminatory efficiency and ability values were 17.24% and 16.66%, respectively.”
t0“MAIZE”
Claim · model-written summary
Least discriminatory ability was 16.66%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The least discriminatory efficiency and ability values were 17.24% and 16.66%, respectively.”
t0“MAIZE”
Claim · model-written summary
UPGMA separated maize lines into two groups.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the maize lines were separated into two groups, i.e., A, B, secondary, and sub-secondary groups.”
Claim · model-written summary
HS and S-10 had the highest genetic similarity, with Nei and Li coefficient 0.23.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest genetic similarity (with the lowest genetic divergence) was estimated according to the Nei and Li coefficient of 0.23 between the two lines HS and S-10.”
t0“MAIZE”