Paper · 2023 · International Journal of Environment and Climate Change

Isolation and Characterization of Bacterial Endophytes from Maize Genotypes Varying in Resistance against Macrophomina phaseolina

Y. Pavani; B. Mallaiah; V. Ramya; et al.

7
claims
15
verbatim quotes
0
citations
1
research areas
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Open access

Abstract

The present work aims to isolate and characterize the bacterial endophytes associated with different maize genotypes varying in resistance to disease reaction against Macrophomina phaseolina. A total of 50 endophytic bacteria were isolated from root and stem of healthy maize plants at 30 DAS, 60 DAS by using two different media like TSA, NA medium and maximum number of endophytic bacterial population were recovered from root followed by stem in all the genotypes and TSA medium was found to be the most suitable medium for deciphering maximum endophytic bacterial diversity. The isolated bacterial endophytes were characterized on the basis of morphological parameters viz., size, shape, colour, margin and texture, elevation, gram staining reaction and it was observed that Gram positive bacteria (68.0 %) formed the dominant group. The colony characterization revealed that circular forms (38.0 %) were dominated. Among the colony features the colonies with entire margins (66.0 %) and convex elevation (44.0 %) were found to be dominating.

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

A total of 50 endophytic bacteria were isolated from maize root and stem.

Taxon
maizeZea mayse1
Organ / stage
root and steme1
Interactor
endophytic bacteriae1symbiont
Outcome
endophytic bacteriae1
Value
50e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“bacterial endophytes associated with different maize genotypes”

✓ located verbatim in the source · abstract, characters 54–116 · show in text ↑
e1

“A total of 50 endophytic bacteria were isolated from root and stem of healthy maize plants at 30 DAS, 60 DAS”

✓ located verbatim in the source · abstract, characters 192–300 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

TSA medium was the most suitable of two media for recovering maize endophytic bacterial diversity.

Taxon
maizeZea mayse0
Treatment
TSA mediume3
Outcome
endophytic bacterial diversitye3
Direction
most suitablee3
Comparator
NA mediume4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“bacterial endophytes associated with different maize genotypes”

✓ located verbatim in the source · abstract, characters 54–116 · show in text ↑
e3

“TSA medium was found to be the most suitable medium for deciphering maximum endophytic bacterial diversity”

✓ located verbatim in the source · abstract, characters 471–577 · show in text ↑
e4

“two different media like TSA, NA medium”

✓ located verbatim in the source · abstract, characters 310–349 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The maximum endophytic bacterial population was recovered from maize root, followed by stem.

Taxon
maizeZea mayse0
Organ / stage
roote2
Outcome
endophytic bacterial populatione2
Direction
maximume2
Comparator
steme2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“bacterial endophytes associated with different maize genotypes”

✓ located verbatim in the source · abstract, characters 54–116 · show in text ↑
e2

“maximum number of endophytic bacterial population were recovered from root followed by stem in all the genotypes”

✓ located verbatim in the source · abstract, characters 354–466 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Gram positive bacteria formed the dominant group among the maize endophytes, at 68.0 %.

Taxon
maizeZea mayse0
Interactor
Gram positive bacteriae5symbiont
Outcome
Gram positive bacteriae5
Value
68.0 %e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“bacterial endophytes associated with different maize genotypes”

✓ located verbatim in the source · abstract, characters 54–116 · show in text ↑
e5

“Gram positive bacteria (68.0 %) formed the dominant group”

✓ located verbatim in the source · abstract, characters 779–836 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Circular colony forms dominated the maize endophytes, at 38.0 %.

Taxon
maizeZea mayse0
Outcome
circular formse6
Value
38.0 %e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“bacterial endophytes associated with different maize genotypes”

✓ located verbatim in the source · abstract, characters 54–116 · show in text ↑
e6

“circular forms (38.0 %) were dominated”

✓ located verbatim in the source · abstract, characters 880–918 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Colonies with entire margins were dominant among the maize endophytes, at 66.0 %.

Taxon
maizeZea mayse0
Outcome
entire marginse7
Value
66.0 %e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“bacterial endophytes associated with different maize genotypes”

✓ located verbatim in the source · abstract, characters 54–116 · show in text ↑
e7

“colonies with entire margins (66.0 %)”

✓ located verbatim in the source · abstract, characters 950–987 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Convex elevation was dominant among the maize endophyte colonies, at 44.0 %.

Taxon
maizeZea mayse0
Outcome
convex elevatione7
Value
44.0 %e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“bacterial endophytes associated with different maize genotypes”

✓ located verbatim in the source · abstract, characters 54–116 · show in text ↑
e7

“convex elevation (44.0 %) were found to be dominating”

✓ located verbatim in the source · abstract, characters 992–1045 · show in text ↑

in vitro · soil: biology · Zea mays (maize)Results agree

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